發(fā)布時(shí)間：2018-05-06 17:48

  本文選題：停循環(huán) + 深低溫��； 參考：《中華實(shí)用診斷與治療雜志》2016年12期

【摘要】：目的探討深低溫停循環(huán)大鼠模型海馬線粒體通透性轉(zhuǎn)換孔的開(kāi)閉情況,及深低溫對(duì)腦保護(hù)的作用機(jī)制。方法21只大鼠隨機(jī)分為深低溫停循環(huán)組、常溫停循環(huán)組和對(duì)照組,每組7只。深低溫停循環(huán)組大鼠體溫降至20℃以下體外循環(huán)10min,維持深低溫條件下停循環(huán)10min;常溫停循環(huán)組大鼠常溫體外循環(huán)10min,停循環(huán)10min;對(duì)照組大鼠常溫體外循環(huán)20min,不降溫不停循環(huán)。采用酯化鈣黃綠素鈷熒光技術(shù)檢測(cè)3組大鼠海馬組織熒光染色情況,采用Western blot法檢測(cè)海馬線粒體細(xì)胞色素C表達(dá)情況。結(jié)果對(duì)照組海馬組織熒光亮度最強(qiáng),常溫停循環(huán)組熒光亮度最弱,深低溫停循環(huán)組熒光亮度介于對(duì)照組和常溫停循環(huán)組之間;深低溫停循環(huán)組和對(duì)照組大鼠海馬組織熒光強(qiáng)度值(1 387.9±19.3、1 774.6±48.3)和線粒體細(xì)胞色素C灰度值比(16.82±1.47、23.54±1.86)高于常溫停循環(huán)組(1 125.6±37.4、12.31±1.43)(P0.05)。結(jié)論停循環(huán)能使大鼠海馬線粒體通透性轉(zhuǎn)換孔開(kāi)放增加,深低溫能降低海馬線粒體通透性轉(zhuǎn)換孔的開(kāi)放數(shù)量,具有腦保護(hù)作用。
[Abstract]:Objective to investigate the opening and closing of mitochondrial permeability transition pore in hippocampus of rat model of deep hypothermia circulatory arrest and the protective mechanism of deep hypothermia on brain. Methods Twenty-one rats were randomly divided into deep hypothermia circulatory arrest group, normothermic circulatory arrest group and control group with 7 rats in each group. The body temperature of the rats in the deep hypothermia cardiopulmonary bypass group was decreased to below 20 鈩，

本文編號(hào)：1853266