本文選題：肝郁脾虛證 + 藥效評價指標�。� 參考：《延邊大學》2012年碩士論文

【摘要】：目的：通過皮下注射四氯化碳、慢性束縛嘗試建立肝郁脾虛證模型,并用經典治療藥逍遙丸化裁方對該模型進行干預,初步形成慢性肝炎肝郁脾虛證的建立方法,形成綜合藥效評價指標。 方法：成年雄性大鼠32只,體重250g-300g。購入大鼠后適應性飼養(yǎng)一周后,分為A(空白組),B(模型組),C(逍遙丸化裁方組)三組�？瞻捉M8只其余每組12只,除A組大鼠外,其余24只大鼠均按0.5ml/100g體重皮下注射10%CCL4豆油溶液,首次劑量加倍,每周注射2次,共四周。第四周從模型組中任選兩只大鼠測ALT、AST來確定肝損傷的程度。開始造模一周后,以慢性束縛方法制作慢性應激大鼠模型,將B、C組大鼠束縛于束縛筒內,放入飼養(yǎng)箱中,每日3h,早8點到11點,連續(xù)21d。A組不予束縛,但于相同時間點禁食,放置于各自飼養(yǎng)箱中3h/d,連續(xù)21d。C組大鼠在實驗開始1周后,每次開始束縛前30mmin給各組大鼠灌胃給藥,1次/d,共用3周。(以人用藥量的30倍作為大鼠給藥的逍遙丸化裁方組,灌胃容積為20mL/kg體重)。A組則分別灌服同等換算體積的蒸餾水。觀察實驗動物的情緒行為、日飲用量、大便溏薄觀察、自主活動能力。給藥后每周灌服D-木糖接取尿液檢測尿液中D-木糖排泄率。檢測血清中5-羥色胺(5-HT)、去甲腎上腺素(NE)、多巴胺(DA)水平,丙氨酸氨基轉移酶(ALT)和門冬氨酸氨基轉移酶(AST)的水平,血漿生長抑素(SS)、胃動素(MTL)、血管活性腸肽(VIP)的水平,總膽紅素(TBILI)、直接膽紅素(DBILI)、總蛋白(TP)、白蛋白(ALB)的變化,白介素-2、6、8的水平。觀察肝臟病理變化。所有指標進行組間t檢驗統(tǒng)計學處理。 結果：行為學指標：實驗動物出現便溏、飲食飲水量減少、暴躁易怒、體重降低等癥狀。逍遙丸化裁方組與模型組比較,肝臟、脾臟、腎上腺指數降低。生化指標顯示與模型組比較：1.空白組、逍遙丸化裁方組尿液中D-木糖的含量低于模型組。2.5-HT含量升高(P0.05),DA明顯升高(P0.01),NE含量又降低趨勢。3.血清中ALT和AST的水平明顯低于模型組(P0.05)。4.ALB明顯升高(P0.001)、DBLI有降低趨勢、TP明顯升高(P0.05),TBILl各組間無明顯差異。5.MTL明顯升高(P0.05)、VIP顯著升高(P0.001)、SS升高(P0.05)。 6.IL-2降低(P0.05)、IL-6降低、IL-8降低。7.空白組肝組織結構正常,模型組肝小葉結構不清,可見明顯的片狀壞死,伴有炎癥細胞浸潤。逍遙丸組肝小葉結構尚正常,可見明顯的混濁腫脹氣球樣變或脂肪變性,散在點狀壞死。 結論：肝炎肝郁脾虛證動物模型綜合藥效評價指標的建立。
[Abstract]:Objective: to establish the model of liver stagnation and spleen deficiency by subcutaneous injection of carbon tetrachloride (CCL 4), and interfere with Xiaoyao Pill (Xiaoyao Pill), a classical therapeutic drug, in order to form the method of establishing liver stagnation and spleen deficiency syndrome in chronic hepatitis. A comprehensive evaluation index of drug efficacy was formed. Methods: 32 adult male rats were weight 250 g-300 g. After a week of adaptive feeding, rats were divided into three groups: group A (model group). Except for group A, the other 24 rats were subcutaneously injected with 10L4 soybean oil solution according to the body weight of 0.5ml/100g. The first dose was doubled, twice a week, for a total of four weeks. In the fourth week, two rats were selected from the model group to determine the degree of liver injury. One week after the model was established, the chronic stress rat model was made by chronic restraint method. The rats of group C were tied to the restraint tube and put into the feeding box for 3 hours daily, 8: 00 to 11:00 in the morning. The rats in the 21d.A group were not bound, but fasted at the same time. The rats in the 21d.C group were placed in their respective feeding boxes for 3 h / d. The rats in the 21d.C group were given intragastric administration of 30mmin for 3 weeks after one week of experiment and before each group began to be bound. (the group of Xiaoyao pills treated with Xiaoyao Pill, 30 times of the dosage of human medicine) was given distilled water with the same volume of distilled water in the group with 20mL/kg body weight. Observe the emotional behavior of experimental animals, daily consumption, loose stool observation, independent activity. The excretion rate of D-xylose in urine was determined by weekly administration of D-xylose. The levels of serotonin 5-HT, norepinephrine (NE), dopamine (DA), alanine aminotransferase (alt) and aspartate aminotransferase (AST), plasma somatostatin (SS), motilin (MTL), vasoactive intestinal peptide (VIP) were measured. The changes of total bilirubin (TBILI), direct bilirubin (DBILI), total protein (TPN), albumin (ALB), and the level of interleukin-2 (TBILI). Liver pathological changes were observed. All the indexes were analyzed by t-test. Results: behavioral indicators: loose stools, decreased diet, irritability, weight loss and so on. Compared with model group, the index of liver, spleen and adrenal gland decreased in Xiaoyao pill group. The biochemical index display was compared with that of the model group. In blank group, the content of Dxylose in urine of Xiaoyao pill group was lower than that of model group. The serum levels of ALT and AST were significantly lower than those of the model group (P 0.05). 4. The levels of ALT and AST in serum were significantly lower than those in the model group. There was a tendency to decrease the level of P0.001TBI. There was no significant difference between the three groups. 5. The levels of ALT and AST were significantly higher than those of the model group. 6.IL-2 decreased P0.05, IL-6 decreased, IL-8 decreased. In blank group, the structure of liver tissue was normal, the structure of hepatic lobule in model group was not clear, obvious flake necrosis and inflammatory cell infiltration could be seen. In Xiaoyao Pill group, the structure of hepatic lobule was normal, obvious turbid swelling, balloon degeneration or steatosis, scattered necrosis. Conclusion: the animal model of liver stagnation and spleen deficiency syndrome was established.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R-332

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