本文選題：Smurf + 慢病毒�。� 參考：《中國比較醫(yī)學雜志》2017年04期

【摘要】：目的構建慢病毒介導穩(wěn)定敲低Smurf1的HeLa和A549細胞株并檢測敲低Smurf1細胞遷移的影響。方法將包裝好的Smurf1敲低慢病毒感染HeLa和A549細胞,7 d后進行嘌呤霉素抗性篩選陽性細胞,Western blot和qPCR檢測敲低效果,并進行Transwell檢測Smurf1敲低對細胞遷移的影響。結果利用干擾慢病毒系統(tǒng)成功構建穩(wěn)定敲低Smurf1的HeLa和A549細胞株,穩(wěn)定敲低Smurf1抑制細胞的遷移速率。結論敲低Smurf1抑制細胞遷移。
[Abstract]:Aim to construct HeLa and A549 cell lines with stable knockout Smurf1 mediated by lentivirus and to detect the migration of Smurf1 knockout cells. Methods HeLa and A549 cells were infected with Smurf1 knockdown virus for 7 days. Western blot and qPCR were used to screen the positive cells for purine mycin resistance, and Transwell was used to detect the effect of Smurf1 knockout on cell migration. Results HeLa and A549 cell lines with stable Smurf1 knockout were successfully constructed by interfering lentivirus system. Stable knock down Smurf1 inhibited the migration rate of the cells. Conclusion knockout of Smurf1 inhibits cell migration.
【作者單位】： 中國醫(yī)學科學院醫(yī)學實驗動物研究所北京協(xié)和醫(yī)學院比較醫(yī)學中心;
【基金】：協(xié)和青年基金資助 中央高校基本科研業(yè)務費專項資金資助(3332016077) 中國醫(yī)學科學院醫(yī)學實驗動物研究所基本科研業(yè)務費專項資助(2016ZX310033)
【分類號】：R329.2
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本文編號：1825330