本文選題：何首烏苷 + 亞急性衰老模型小鼠��； 參考：《四川動(dòng)物》2017年01期

【摘要】：目的研究何首烏苷(Polygonum multiflorum Thunb.glycoside)對(duì)亞急性衰老模型小鼠學(xué)習(xí)記憶能力及海馬組織內(nèi)膽堿乙酰轉(zhuǎn)移酶(ChAT)和乙酰膽堿酯酶(AChE)表達(dá)的影響。方法采用AlCl_3(10mg·kg~(-1))灌胃及D-半乳糖(120 mg·kg~(-1))頸部皮下注射,持續(xù)處理60 d井通過跳臺(tái)逃避行為學(xué)實(shí)驗(yàn)法篩選出亞急性衰老KM小鼠模型;將成功構(gòu)建的衰老模型小鼠,雌雄各半,隨機(jī)分為5組,包括:衰老模型組、雌二醇陽性對(duì)照組(10 mg·kg~(-1))和何首烏苷高、中、低劑量組(劑量分別為:80mg·kg~(-1)、40 mg·kg~(-1)、20 mg·kg~(-1));并選擇健康小鼠作為正常對(duì)照組。治療組小鼠在造模后15 d起分別頸部皮下注射高、中、低劑量的何首烏苷和雌二醇,持續(xù)給藥15 d;衰老模型組和正常對(duì)照組小鼠分別給予等體積生理鹽水。利用跳臺(tái)逃避行為學(xué)實(shí)驗(yàn)法判斷各組小鼠的學(xué)習(xí)記憶能力的變化和差異;利用Western Blot法檢測(cè)小鼠海馬體組織細(xì)胞內(nèi)膽堿酯酶系統(tǒng)ChAT和AChE蛋白表達(dá)情況。結(jié)果與正常對(duì)照組比較,各實(shí)驗(yàn)組小鼠學(xué)習(xí)記憶能力顯著下降,小鼠跳臺(tái)回避錯(cuò)誤次數(shù)顯著增加(P0.05);與衰老模型組比較,不同劑量何首烏苷組與雌二醇陽性對(duì)照組小鼠學(xué)習(xí)記憶能力明顯改善,小鼠跳臺(tái)回避錯(cuò)誤次數(shù)明顯減少(P0.05或P0.001),而不同劑量何首烏苷組與雌二醇陽性對(duì)照組小鼠跳臺(tái)回避錯(cuò)誤次數(shù)相近(P0.05);與衰老模型組比較,不同劑量何首烏苷組與雌二醇陽性對(duì)照組小鼠海馬體組織細(xì)胞內(nèi)的ChAT表達(dá)量升高而AChE表達(dá)量下降,差異有統(tǒng)計(jì)學(xué)意義或高度統(tǒng)計(jì)學(xué)意義(P0.05或P0.001)。結(jié)論何首烏苷能有效改善亞急性衰老模型小鼠的學(xué)習(xí)記憶能力障礙,其機(jī)制與其使海馬內(nèi)組織細(xì)胞的ChAT表達(dá)升高和AChE表達(dá)降低,從而增加乙酰膽堿的含量以改善膽堿能系統(tǒng)損害有關(guān)。
[Abstract]:Objective to study the effects of Polygonum multiflorum Thunb.glycoside on learning and memory ability and the expression of choline acetyltransferase (ChATs) and acetylcholinesterase (ache) in hippocampal tissues of subacute aging mice. Methods the model of subacute aging km mice was selected by subcutaneous injection of AlCl_3(10mg and Dgalactose (120mg / kg) into the neck for 60 days, and the model of subacute aging km mice was selected by escape behavior experiment, and the model mice were divided into two groups, half male and half male, half male and half male. Five groups were randomly divided into five groups: aging model group, estradiol positive control group (estradiol positive control group, 10 mg 路kg ~ (-1), high, middle and low dose groups (40 mg 路kg ~ (-1) (40 mg 路kg ~ (-1) and 20 mg 路kg ~ (-1) C ~ (-1), respectively, and healthy mice were selected as normal control group. Mice in the treatment group were subcutaneously injected with high, medium and low doses of Polygonum multiflorum and estradiol for 15 days, respectively, and mice in the aging model group and the normal control group were given the same volume of normal saline respectively. The changes and differences of learning and memory ability in each group were evaluated by means of escape behavior experiment, and the expression of cholinesterase system (ChAT) and AChE protein in hippocampal tissue of mice were detected by Western Blot method. Results compared with the normal control group, the learning and memory ability of the mice in each experimental group was significantly decreased, and the number of errors in jumping platform avoidance was significantly increased in the mice compared with the aging model group. The learning and memory ability of mice in different dosage groups of Polygonum multiflorum and estradiol positive control group was improved obviously. The number of error-avoidance errors of platform avoidance in mice was significantly decreased by P0.05 or P0.001g, while the times of avoidance errors in mice with different doses of Polygonum multiflorum were similar to those of mice with estradiol positive control group (P 0.05), which were compared with those of aging model group. The expression of ChAT in hippocampal tissue of different dose group and estradiol positive control group increased but AChE expression decreased, the difference was statistically significant or highly statistically significant (P0.05 or P0.001). Conclusion Polygonum multiflorum glycoside can effectively improve the learning and memory ability of subacute aging model mice, and its mechanism is related to the increase of ChAT expression and the decrease of AChE expression in hippocampal tissue cells. It is related to increase the content of acetylcholine to improve the damage of cholinergic system.
【作者單位】： 中央民族大學(xué)生命與環(huán)境科學(xué)學(xué)院;中央民族大學(xué)北京市食品環(huán)境與健康工程技術(shù)研究中心;中央民族大學(xué)醫(yī)院檢驗(yàn)科;
【基金】：國家自然科學(xué)基金項(xiàng)目(31372225) 中央高校自主科研基金項(xiàng)目(2016SHXY01) 社會(huì)醫(yī)學(xué)學(xué)術(shù)團(tuán)隊(duì)建設(shè)項(xiàng)目(2015MDTD13C) 北京市大學(xué)生創(chuàng)新項(xiàng)目(URTP2015110019)
【分類號(hào)】：R285.5;R-332

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