本文選題：腎脂肪囊 + 脂肪干細胞��； 參考：《細胞與分子免疫學雜志》2010年11期

【摘要】：目的:研究腎脂肪囊內(nèi)脂肪干細胞(ADSCs)的培養(yǎng)條件及生物學特性,為干細胞治療腎臟疾病尋找更佳的細胞來源。方法:分別取大鼠腎脂肪囊及腹股溝區(qū)脂肪組織,分離培養(yǎng)ADSCs進行對比,細胞達80%融合時傳代,觀察細胞形態(tài)和增殖情況;采用MTT比色法測定細胞生長曲線;采用流式細胞術(shù)(FCM)檢測細胞表面抗原的表達。結(jié)果:①相同質(zhì)量腎脂肪囊和腹股溝區(qū)脂肪組織分離培養(yǎng)原代ADSCs顯示,腎脂肪囊分離出ADSCs的數(shù)量顯著超過腹股溝區(qū)脂肪組織;腎脂肪囊來源的ADSCs 5～6 d可達80%融合,而腹股溝區(qū)脂肪來源ADSCs則需要10～12 d。兩者細胞均成梭形成纖維樣細胞形態(tài)。②生長曲線分析顯示腎脂肪囊ADSCs較腹股溝區(qū)ADSCs增殖迅速。③流式細胞術(shù)檢測示兩種干細胞的CD29抗原表達率均99%,腎脂肪囊ADSCs CD44表達率為52.92%,腹股溝區(qū)ADSCs表達率為75.41%,內(nèi)皮細胞標志抗原CD31均陰性。結(jié)論:與其他部位來源ADSCs相比,腎脂肪囊來源ADSCs具有分離量大,增殖迅速等特點,可在短時間內(nèi)擴增到較大數(shù)量,可能成為治療腎臟疾病的新的干細胞來源。
[Abstract]:Aim: to study the culture conditions and biological characteristics of adipose stem cells (ADSCs) in renal fat sac and to find a better cell source for the treatment of renal diseases by stem cells. Methods: the adipose tissue of rat kidney fat sac and inguinal area were isolated and cultured for comparison. When the cells reached 80% fusion, the morphology and proliferation of the cells were observed, and the growth curve of the cells was measured by MTT colorimetry. Flow cytometry (FCM) was used to detect the expression of cell surface antigen. Results the primary ADSCs isolated from the same mass of renal fat sac and inguinal adipose tissue showed that the number of ADSCs isolated from renal fat sac was significantly higher than that of inguinal adipose tissue, and 80% of ADSCs from renal fat sac could be fused for 6 days. However, ADSCs of inguinal area fat source takes 10 ~ 12 days. The morphological and growth curve analysis of both fusiform fibroblasts showed that the proliferation of renal fat sac ADSCs was faster than that of groin ADSCs. 3. 3 flow cytometry showed that the expression rate of CD29 antigen of the two stem cells was 99%, and that of renal adipose sac ADSCs was 99%. The expression rate of CD44 was 52.92%, the expression rate of ADSCs in inguinal area was 75.41, and the CD31 of endothelial marker antigen was negative. Conclusion: compared with other sources of ADSCs, ADSCs derived from renal fat sac has the characteristics of large amount of isolation and rapid proliferation, and can be expanded in a large number in a short time, which may become a new source of stem cells for the treatment of renal diseases.
【作者單位】： 遼寧醫(yī)學院腎臟病科;沈陽軍區(qū)總醫(yī)院腎病科;
【分類號】：R692

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本文編號：1798303