本文選題：高血壓病 + 痰濕壅盛　； 參考：《中醫(yī)雜志》2017年13期

【摘要】：目的探討半夏白術天麻湯治療痰濕壅盛型高血壓病的可能分子機制。方法大鼠高脂飲食24周建立痰濕壅盛型高血壓模型,16只造模成功大鼠隨機分為模型組和半夏白術天麻湯組,每組各8只,并以普通飼料喂養(yǎng)大鼠8只設為正常組。半夏白術天麻湯組給予半夏白術天麻湯13.8 g/(kg·d)灌胃,正常組和模型組給予等量生理鹽水灌胃,均灌胃12周。記錄大鼠體重、血壓,計算Lee's指數(shù),檢測大鼠血清葡萄糖、甘油三酯、總膽固醇水平;雙向電泳分離樣品并且進行硝酸銀染色,篩選下丘腦差異蛋白質點提交質譜鑒定,采用MALDI-TOF-TOF質譜儀進行質譜分析、肽指紋圖譜進行蛋白質鑒定,選取部分蛋白質點驗證蛋白質組學結果。結果半夏白術天麻湯組大鼠體重、血壓、Lee's指數(shù)、血清總膽固醇均較模型組下降(P0.05)。正常組、模型組、半夏白術天麻湯組分別分離出3342、3844、3828個蛋白點,其中半夏白術天麻湯組同模型組比較,100個蛋白質表達降低,72個蛋白質表達增加。在質譜儀進一步鑒定的19種蛋白中,模型組與正常組相比有差異而半夏白術天麻湯可使差異趨勢逆轉的蛋白點為11個,其中5個表達上調,6個表達下調。結論半夏白術天麻湯治療痰濕壅盛型高血壓病的分子機制可能與改善細胞骨架和形態(tài)、內質網應激、能量代謝、神經元結構等有關。
[Abstract]:Objective to explore the possible molecular mechanism of Banxia Atractylodes Tianma decoction in the treatment of hypertensives with phlegm and dampness. Methods Sixteen rats were randomly divided into two groups: model group and Banxia Atractylodes Tianma decoction group, with 8 rats in each group, and 8 rats were fed with normal diet as normal group. Banxia Atractylodes Tianma decoction group was given Banxia Atractylodes Tianma decoction 13.8 g/(kg d), normal group and model group were given the same amount of normal saline for 12 weeks. Body weight, blood pressure, Lee's index, serum glucose, triglyceride and total cholesterol levels were measured, samples were separated by two-dimensional electrophoresis and silver nitrate staining was performed to screen hypothalamic differential protein spots for mass spectrometry. MALDI-TOF-TOF mass spectrometer was used for mass spectrometry, peptide fingerprint was used for protein identification, and some protein spots were selected to verify the proteomic results. Results the body weight, blood pressure and serum total cholesterol of Banxia Atractylodes Tianma decoction group were lower than that of model group (P 0.05). In normal group, model group and Banxia Atractylodes Tianma decoction group, 3342 protein spots were isolated, 3828 protein spots were isolated respectively. Compared with model group, 100 protein expression decreased and 72 protein expression increased in Banxia Atractylodes Tianma decoction group. Among the 19 proteins further identified by mass spectrometer, there were differences between the model group and the normal group, while the protein points of Banxia Atractylodes Tianma decoction could reverse the trend of the difference were 11, among which 5 were up-regulated and 6 down-regulated. Conclusion the molecular mechanism of Banxia Atractylodes Tianma decoction in the treatment of hypertensives with phlegm-dampness accumulation may be related to the improvement of cytoskeleton and morphology, endoplasmic reticulum stress, energy metabolism and neuronal structure.
【作者單位】： 山東中醫(yī)藥大學第一臨床醫(yī)學院;山東中醫(yī)藥大學附屬醫(yī)院;青島海慈集團;
【基金】：國家自然科學基金(81573916,81673807)
【分類號】：R285.5;R-332

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