發(fā)布時(shí)間：2018-04-23 00:02

  本文選題：松節(jié)油 + 豚鼠��； 參考：《大理學(xué)院》2012年碩士論文

【摘要】：第一部分松節(jié)油肝內(nèi)注射的劑量及實(shí)驗(yàn)動物的初步探討 材料與方法 豚鼠6只，質(zhì)量在350~420g之間，雌性，由大理學(xué)院實(shí)驗(yàn)動物中心提供。隨機(jī)分成三組：A組為手術(shù)組，開腹手術(shù)，切除部分肝臟；另一只行肝門部阻滯；B組為開腹肝臟穿刺注射松節(jié)油；C組為超聲下經(jīng)皮穿刺注射松節(jié)油。 結(jié)果 1A組一只豚鼠在術(shù)后關(guān)腹腔時(shí)，死亡。另一只在術(shù)中解除肝阻滯約5分鐘后死亡。 2B組注射松節(jié)油0.3ml的豚鼠在術(shù)后24小時(shí)后死亡。第二只注射0.1ml的豚鼠麻醉后沒有蘇醒，死亡。 3C組在固定但沒有麻醉的情況下，一只豚鼠在經(jīng)皮肝穿刺時(shí)瞬間劇烈掙扎，致使穿刺針在胸腹腔內(nèi)攪動，隨即停止呼吸與心跳。另一只麻醉后，超聲監(jiān)測困難，穿刺出血多，只好放棄。 結(jié)論 1將松節(jié)油注射于肝臟制作局部炎癥時(shí)，豚鼠對松節(jié)油過于敏感，死亡率太高。 2豚鼠不適合常規(guī)下作經(jīng)皮肝穿刺的實(shí)驗(yàn)動物。 3豚鼠創(chuàng)傷耐受性差，不利于術(shù)后的康復(fù)及實(shí)驗(yàn)觀察，進(jìn)一步改進(jìn)實(shí)驗(yàn)！ 第二部分松節(jié)油、葡萄球菌混懸液肝內(nèi)注射對兔肝組織作用的實(shí) 驗(yàn)研究 材料與方法 本地成年健康家兔12只，質(zhì)量在1.90~2.20kg之間，雌性，，由大理學(xué)院實(shí)驗(yàn)動物中心提供。隨機(jī)分成四組：A組為對照組：無水酒精組；B組為松節(jié)油組；C組為葡萄球菌組；D組松節(jié)油、葡萄球菌混液系列組。 結(jié)果 1A組為對照組：無菌生理鹽水組，三只家兔中肝臟注射部位都無特殊變化。 2B組為松節(jié)油組，三只家兔中，有兩只肝臟注射部位形成膿腫，一只出現(xiàn)局部炎癥； 3C組為金黃色葡萄球菌組，有兩只肝臟注射部位形成炎癥，一只出現(xiàn)局部膿腫； 4D組為松節(jié)油-金黃色葡萄球菌懸液組，三只家兔中兩只肝臟注射部位全部形成膿腫； 5十二只家兔，全部存活，肝臟出現(xiàn)膿腫或者炎癥的，抗生素藥物能控制。 結(jié)論 1家兔作為經(jīng)皮肝穿刺的實(shí)驗(yàn)動物、肝臟瘤內(nèi)注射炎性制劑是安全的。 2松節(jié)油與葡萄球菌懸液序貫性瘤內(nèi)注射可以使局部肝組織壞死、化膿；是理想的炎性制劑。 3肝臟瘤內(nèi)內(nèi)注射炎性制劑治療腫瘤可能成為一種治療模式！
[Abstract]:Part I preliminary study on intrahepatic injection of turpentine and experimental animals Materials and methods Six guinea pigs, weighing between 350 and 420 g, were provided by the Experimental Animal Center of Dali University. The rats were randomly divided into three groups: operation group (n = 3). Result One guinea pig in group 1A died after operation. The other died about 5 minutes after the liver block was lifted. Guinea pigs injected with turpentine 0.3ml died 24 hours after operation. The second guinea pig injected with 0.1ml did not wake up and died after anesthesia. In the 3C group, a guinea pig struggled violently during the percutaneous liver puncture, which caused the needle to stir in the chest and abdominal cavity, and then stopped breathing and heartbeat. Another anesthetized, ultrasound monitoring difficulties, puncture bleeding, had to give up. Conclusion 1 when the turpentine was injected into the liver to make local inflammation, the guinea pig was too sensitive to turpentine and the mortality was too high. 2 Guinea pigs are not suitable for routine percutaneous liver puncture. (3) the poor tolerance to trauma in guinea pigs is not conducive to postoperative rehabilitation and experimental observation, and further improvement of the experiment! The second part: the effect of intrahepatic injection of turpentine and staphylococcal suspension on rabbit liver tissue Empirical study Materials and methods Twelve local adult healthy rabbits, with mass between 1.90~2.20kg and female, were provided by the Experimental Animal Center of Dali University. The rats were randomly divided into four groups: control group: anhydrous alcohol group B group: turpentine group C group Staphylococcus group D group turpentine group and staphylococcal mixture group. Result Group 1A: control group: no special changes were found in the injection site of liver in three rabbits in aseptic saline group. Group 2B was turpentine group, two of the three rabbits had abscess at the injection site of liver and one had local inflammation. 3C group was staphylococcus aureus group, two livers were injected with inflammation and one had local abscess. 4Group D was turpentine-Staphylococcus aureus suspension group, and two of the three rabbits had abscess at the injection site of liver. Five-twelve rabbits, all alive, liver abscess or inflammation, antibiotics can be controlled. Conclusion 1 as the experimental animal of percutaneous liver puncture, it is safe to inject inflammatory agent into the liver. 2Sequential intratumoral injection of turpentine and staphylococcal suspension can make local liver tissue necrotic and suppurate, and is an ideal inflammatory preparation. 3Intrahepatic injection of inflammatory agents in the treatment of liver tumors may become a therapeutic model!
【學(xué)位授予單位】：大理學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R657.3;R-332

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