本文選題：β-Klotho + 受體��； 參考：《中國循環(huán)雜志》2017年05期

【摘要】：目的:觀察血管鈣化對(duì)大鼠腎功能的損傷情況,及其與腎臟組織局部β-Klotho[成纖維細(xì)胞生長因子21(FGF21)的輔助因子]、成纖維細(xì)胞生長因子受體1(FGFR1)表達(dá)變化關(guān)系,探討β-Klotho及FGFR1在血管鈣化腎臟損傷中的作用和意義。方法:實(shí)驗(yàn)動(dòng)物按電腦隨機(jī)數(shù)字表法分為正常對(duì)照組和鈣化組,每組6只。鈣化組采用維生素D3聯(lián)合尼古丁誘導(dǎo)大鼠血管鈣化模型。以肌氨酸氧化酶法檢測大鼠血清肌酐濃度,以紫外-谷氨酸脫氫酶法檢測大鼠血清尿素氮濃度,以生化法檢測大鼠血鈣及血磷濃度,以堿性磷酸酶(ALP)試劑盒檢測大鼠腎臟組織ALP活性,以酶聯(lián)免疫吸附法檢測大鼠腎臟組織β-Klotho及FGFR1蛋白含量。結(jié)果:與正常對(duì)照組比較,血管鈣化組大鼠血清肌酐及尿素氮水平升高[(35.200±4.087)μmol/L vs(26.000±5.0990)μmol/L,(P0.05);(6.900±0.623)mmol/L vs(5.400±0.803)mmol/L,(P0.05)];大鼠腎臟組織局部ALP活性升高[(60.510±31.090)U/g vs(26.590±8.664)U/g,(P0.05)];腎臟組織β-Klotho蛋白表達(dá)量增加[(9.052±1.238)ng/mg vs(6.860±1.036)ng/mg,(P0.05)],而血鈣及血磷濃度及大鼠腎臟組織FGFR1蛋白含量較正常對(duì)照組未見明顯變化。結(jié)論:大劑量維生素D3肌肉注射聯(lián)合尼古丁灌胃可導(dǎo)致大鼠腎臟組織局部鈣超載及微血管鈣化形成,并導(dǎo)致腎功能不全,出現(xiàn)早期慢性腎臟病(CKD)臨床表現(xiàn)。同時(shí)病變腎臟組織局部β-Klotho表達(dá)量上調(diào),而FGFR1在腎臟組織局部未見明顯變化,提示FGF21在血管鈣化大鼠腎臟組織局部的調(diào)節(jié)作用可能不是通過增加其受體FGFR1的數(shù)量,而是通過上調(diào)其結(jié)合輔助因子β-Klotho的表達(dá)而實(shí)現(xiàn)的。同時(shí)提示β-Klotho參與了血管鈣化腎臟損傷的早期調(diào)節(jié)過程,可能為預(yù)測機(jī)體鈣超載情況及CKD早期的預(yù)警信號(hào),對(duì)CKD的早期診斷具有一定價(jià)值。
[Abstract]:Objective: to observe the damage of vascular calcification to renal function in rats, and its relationship with the expression of 尾 -Klotho (fibroblast growth factor 21FGF21) and fibroblast growth factor receptor (FGFR1). To investigate the role and significance of 尾-Klotho and FGFR1 in calcified kidney injury. Methods: experimental animals were randomly divided into normal control group and calcified group with 6 rats in each group. Vascular calcification model was induced by vitamin D 3 combined with nicotine in calcification group. Serum creatinine concentration was detected by creatine oxidase method, serum urea nitrogen concentration was detected by UV-glutamate dehydrogenase method, and serum calcium and phosphorus concentrations were detected by biochemical method. Alkaline phosphatase (ALP) kit was used to detect the activity of ALP in rat kidney and the content of 尾 -Klotho and FGFR1 protein in renal tissue of rats was detected by enzyme-linked immunosorbent assay (Elisa). Results: compared with the normal control group, 琛，

本文編號(hào)：1779624