發(fā)布時(shí)間：2018-04-14 23:39

  本文選題：慢性睪丸痛 + 背根神經(jīng)節(jié)��； 參考：《中華男科學(xué)雜志》2017年04期

【摘要】：目的:探討瞬時(shí)受體電位通道蛋白V1(TRPV1)和瞬時(shí)受體電位通道蛋白A1(TRPA1)在睪丸痛大鼠模型的表達(dá)及作用機(jī)制。方法:通過(guò)睪丸內(nèi)注射2%醋酸的方法建立睪丸痛大鼠模型;造模后通過(guò)行為學(xué)實(shí)驗(yàn)檢測(cè)大鼠自發(fā)痛計(jì)數(shù)與陰囊部熱痛閾;RT-q PCR、Western印跡、免疫熒光檢測(cè)T_(13)~L_1節(jié)段背根神經(jīng)節(jié)(DRG)TRPV1和TRPA1的表達(dá)。結(jié)果:建模后大鼠自發(fā)痛計(jì)數(shù)較對(duì)照組明顯增多[(22.63±3.42)次vs(0.13±0.35)次,P0.01],熱痛閾值下降,其下降峰值在注射后第4小時(shí)(4.85±1.00 s),與對(duì)照組(12.75±1.50 s)相比差異有統(tǒng)計(jì)學(xué)意義(P0.01)。TRPV1和TRPA1均表達(dá)在DRG內(nèi)神經(jīng)元的胞膜上,與對(duì)照組相比,模型組大鼠DRG內(nèi)TRPV1的mRNA水平上升至對(duì)照組的(1.77±0.34)倍,TRPA1的mRNA水平上升至對(duì)照組的(1.75±0.30)倍,且兩種蛋白均表達(dá)上調(diào),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:TRPV1和TRPA1可能通過(guò)在DRG內(nèi)表達(dá)上調(diào),參與了睪丸痛大鼠模型的痛覺(jué)產(chǎn)生與外周痛敏的形成。
[Abstract]:Aim: to investigate the expression and mechanism of transient receptor channel protein (V1 / TRPV1) and transient receptor channel protein A1 / TRPA1 in rat model of testicular pain.Methods: the rat model of testicular pain was established by intratesticular injection of 2% acetic acid.The expression of TRPV1 and TRPA1 in dorsal root ganglion of T_(13)~L_1 was detected by immunofluorescence.Results: compared with the control group, the number of spontaneous pain increased [22.63 鹵3.42 times, vs(0.13 鹵0.35 times, P0.01], and the threshold of thermal pain decreased, compared with the control group.The peak value of the decrease was 4.85 鹵1.00 s-1 at the 4th hour after injection and 12.75 鹵1.50 s in the control group. There was a significant difference in the expression of P0.01, TRPV1 and TRPA1 on the membrane of neurons in DRG, and compared with that in the control group.The mRNA level of TRPV1 in the DRG of the model group was 1.77 鹵0.34 times higher than that of the control group, and the mRNA level of TRPA1 was 1.75 鹵0.30 times higher than that of the control group, and the expression of both proteins was up-regulated (P 0.05).Conclusion it is suggested that the up-regulation of TRPA1 and TPV _ 1 may be involved in the generation of pain and peripheral pain sensitivity in testicular pain rat model by up-regulating the expression of TPV _ 1 and TRPA1 in the rat model of testicular pain.
【作者單位】： 中山大學(xué)附屬第一醫(yī)院泌尿男科;中山大學(xué)生命科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(81172432和81471449) 廣東省科技計(jì)劃項(xiàng)目(2016A020218007和2013B051000021) 廣州市科技計(jì)劃項(xiàng)目(2013J4500010)~~
【分類(lèi)號(hào)】：R-332;R697.22
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本文編號(hào)：1751567