本文選題：月經(jīng)血間充質(zhì)干細(xì)胞 + 細(xì)胞培養(yǎng)��； 參考：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年07期

【摘要】：目的探討月經(jīng)血間充質(zhì)干細(xì)胞在體外可否分化為成肌細(xì)胞。方法提取并用10%FBS的DMEM培養(yǎng)液培養(yǎng)人月經(jīng)血間充質(zhì)干細(xì)胞和SD大鼠的肌衛(wèi)星細(xì)胞;誘導(dǎo)組用肌衛(wèi)星細(xì)胞培養(yǎng)的上清液和5-氮雜胞甙對(duì)月經(jīng)血間充質(zhì)干細(xì)胞進(jìn)行誘導(dǎo),對(duì)照組用10%FBS的DMEM培養(yǎng)液培養(yǎng)細(xì)胞;誘導(dǎo)后,用免疫熒光和RT-PCR方法檢測(cè)成肌細(xì)胞標(biāo)記蛋白Pax7蛋白和mRNA的表達(dá)。結(jié)果經(jīng)免疫熒光檢測(cè),誘導(dǎo)組細(xì)胞Pax7陽(yáng)性率為80.36%,與對(duì)照組比較,具有統(tǒng)計(jì)學(xué)意義(F=15.237,P=0.000 1);經(jīng)RT-PCR檢測(cè),誘導(dǎo)組與對(duì)照組比較,Pax7基因呈現(xiàn)具有統(tǒng)計(jì)學(xué)意義的陽(yáng)性表達(dá)(F=14.339,P=0.000 1)。結(jié)論月經(jīng)血間充質(zhì)干細(xì)胞在體外可分化為成肌細(xì)胞。
[Abstract]:Objective to investigate whether menstrual blood mesenchymal stem cells can differentiate into myoblasts in vitro.Methods Human mesenchymal stem cells and myosatellite cells of SD rats were cultured in 10 S DMEM medium, and the mesenchymal stem cells of menstruation were induced by supernatant and 5-azacytidine in the induction group.The cells in control group were cultured in 10s DMEM medium, and the expression of Pax7 protein and mRNA were detected by immunofluorescence and RT-PCR.Results the positive rate of Pax7 in the induced group was 80.36, which was significantly higher than that in the control group (P < 0.01), and the positive expression of Pax7 gene in the induced group was significantly higher than that in the control group (P < 0.01).Conclusion Mesenchymal stem cells can differentiate into myoblasts in vitro.
【作者單位】： 牡丹江醫(yī)學(xué)院體育教研室;牡丹江醫(yī)學(xué)院組織學(xué)與胚胎學(xué)教研室;
【基金】：黑龍江省教育廳科學(xué)技術(shù)研究項(xiàng)目(編號(hào):12531732)
【分類(lèi)號(hào)】：R329.2

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