豬、雞源分離糞腸球菌的核糖體分型及耐藥性分析
本文選題:糞腸球菌 + 耐藥性; 參考:《中國(guó)抗生素雜志》2017年03期
【摘要】:對(duì)上海地區(qū)1個(gè)規(guī);B(yǎng)豬場(chǎng)和1個(gè)規(guī);B(yǎng)雞場(chǎng)分離鑒定的4 0株糞腸球菌,采用微量肉湯稀釋法對(duì)12種抗菌藥物進(jìn)行藥敏試驗(yàn),采用Riboprinter~?微生物基因指紋鑒定系統(tǒng)檢測(cè)分離菌株的基因指紋圖譜,分別檢測(cè)分離菌株中的核糖體型和耐藥性。結(jié)果表明,在40株糞腸球菌中,80%以上的為多重耐藥菌株,有4株豬源糞腸球菌對(duì)萬(wàn)古霉素耐藥,其中有2株表現(xiàn)為對(duì)萬(wàn)古霉素高度耐藥(MIC64mg/L),且對(duì)鏈霉素和慶大霉素同時(shí)表現(xiàn)為高度耐藥(MIC2048mg/L)。從耐藥表型和核糖體分型共同分析糞腸球菌的耐藥性,發(fā)現(xiàn)多重耐藥性嚴(yán)重,同一核糖體型菌株的耐藥表型并非完全一致。
[Abstract]:Forty strains of Enterococcus faecalis isolated and identified from a large scale pig farm and a large scale chicken farm in Shanghai area were tested for drug sensitivity of 12 antimicrobial agents by the method of broth dilution and Riboprinterum?Microorganism gene fingerprint system was used to detect the gene fingerprint of the isolated strain, and the ribosomal type and drug resistance of the isolated strain were detected respectively.The results showed that more than 80% of the 40 strains of Enterococcus faecalis were multidrug resistant, and 4 strains of Enterococcus faecalis were resistant to vancomycin.Two of them showed high resistance to vancomycin, MIC64mg / L, and to streptomycin and gentamicin, they were highly resistant to MIC2048mg / L, respectively.The drug resistance of Enterococcus faecalis was analyzed from the drug resistance phenotype and ribosomal typing. It was found that the multidrug resistance of Enterococcus faecalis was serious and the resistance phenotype of the same ribosomal strain was not identical.
【作者單位】: 上海市獸藥飼料檢測(cè)所;
【基金】:上海市科技興農(nóng)重點(diǎn)攻關(guān)項(xiàng)目(滬農(nóng)科攻字(2015)第6-3-1號(hào))
【分類號(hào)】:S852.61
【相似文獻(xiàn)】
相關(guān)期刊論文 前10條
1 羽曉瑜;陸玉霞;;糞腸球菌聚集物質(zhì)的研究進(jìn)展[J];中國(guó)微生態(tài)學(xué)雜志;2010年07期
2 王睿,方翼,陳遷,白艷紅;糞腸球菌的抗生素后效應(yīng)[J];中國(guó)醫(yī)院藥學(xué)雜志;1999年08期
3 黃雁;劉美容;;福建省某雞場(chǎng)糞腸球菌耐藥情況調(diào)查[J];國(guó)外畜牧學(xué)(豬與禽);2008年04期
4 王穎,陳敏,顧其芳,張美英,周培君;應(yīng)用脈沖凝膠電泳分析糞腸球菌基因的方法研究[J];上海預(yù)防醫(yī)學(xué)雜志;2002年06期
5 劉燕霏;楊建德;趙瑞利;;糞腸球菌動(dòng)物致病的研究進(jìn)展[J];家畜生態(tài)學(xué)報(bào);2013年11期
6 潘偉光;李一凡;鄧啟文;張健華;;1株利奈唑胺耐藥糞腸球菌的基因突變檢測(cè)[J];中國(guó)感染與化療雜志;2013年02期
7 齊亞銀;張莉;王靜梅;崔茹鵬;莫名;剡根強(qiáng);;動(dòng)物源糞腸球菌對(duì)7種抗生素耐藥表型及耐藥基因檢測(cè)[J];中國(guó)獸醫(yī)學(xué)報(bào);2010年07期
8 羅珍蘭,謝繼志,吳峻宇,,吳立軍;乳酸菌膠囊糞腸球菌的分離鑒定及生長(zhǎng)特性觀察[J];江蘇農(nóng)學(xué)院學(xué)報(bào);1996年04期
9 吳利先,黃文祥;糞腸球菌sprE基因的功能研究[J];中華微生物學(xué)和免疫學(xué)雜志;2005年10期
10 孫小平;黃文祥;吳利先;;糞腸球菌基因hyl_1功能的實(shí)驗(yàn)研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2007年14期
相關(guān)會(huì)議論文 前5條
1 劉紅艷;韋曦;凌均h
本文編號(hào):1741584
本文鏈接:http://sikaile.net/xiyixuelunwen/1741584.html