本文選題：薄束核　切入點：Canny邊緣檢測法　出處：《杭州師范大學》2012年碩士論文

【摘要】：從找到人類大腦腦立體定位的方法后,人類腦神經(jīng)科學的研究開始發(fā)展,并應用于臨床疾病治療。人類對腦內(nèi)神經(jīng)核團的研究,從蒼白球、丘腦腹外側(cè)核到杏仁核、豆狀核、尾狀核,研究越來越深入。腦干是眾多神經(jīng)核團的位置所在,但目前對于各個神經(jīng)核團的三維形態(tài)、立體結(jié)構(gòu)、體積大小,都知之甚少。本研究作為腦干神經(jīng)核團研究的起步,探索薄束核的三維形態(tài)與位置。 采用兩例中國成年男性的腦干樣本,依據(jù)各種切片方法不同的優(yōu)缺點,三維重建需要連續(xù)切片,故選擇石蠟切片方法制作切片。把腦干標本分塊,設(shè)置好定位點,依次做好標記,制成蠟塊,進行切片。 腦神經(jīng)組織的染色方法有多種,本研究采用焦油紫染色。把用焦油紫染色染好的片子,放到正置顯微鏡下,每張都按照定位點拍好。 下一步進行圖像校正,其目的是為后來的圖像分割做好準備。圖像分割是提取感興趣的信息,計算機三維重建的必經(jīng)過程。本研究采用自動分割與與手工分割相結(jié)合來進行圖像像分割。自動分割的方法很多,本研究采用的是Canny邊緣檢測法,進行薄束核的自動分割。 然后開始薄束核的三維重建。三維重建的方法,有面重建和體重建兩種,比較兩種方法的優(yōu)缺點后,選擇是面向密集數(shù)據(jù)場的面重建方法,進行薄束核的三維重建。 現(xiàn)有的核團三維指標的測量與統(tǒng)計方法存在缺陷,本研究采用了形狀平均-測量平均的三維指標,對薄束核進行了測量與統(tǒng)計。 經(jīng)以上研究過程,本研究得出三項研究結(jié)果,①得到了薄束核的三維模型,可以以任意角度進行觀察,用任意方位進行切割分析,可以在計算機直觀、形象、逼真地展示薄束核三維形態(tài)和三維位置。②得到了薄束核的三維指標的數(shù)據(jù),表明薄束核是左右兩側(cè)對稱的不規(guī)則核團,右側(cè)比左側(cè)稍大：右側(cè)薄束核長度8.659mm、寬度5.842mm、高度4.897mm,體積67.80mm3。左側(cè)薄束核長度是8.411mm、寬度4.250mm、高度3.560mm,體積51.36mm3。③得出了連續(xù)的、核團界限較清晰的、結(jié)構(gòu)展示較完整的腦干延髓的斷層圖譜。
[Abstract]:The research of human brain neuroscience began to develop after finding the method of brain stereotaxic localization and applied to the treatment of clinical diseases.Human studies on the nucleus of the brain from the globus pallidus ventrolateral nucleus of thalamus to amygdala lentiform nucleus caudate nucleus are getting more and more in-depth.The brain stem is the location of many nerve nuclei, but little is known about the three-dimensional shape, structure and volume of each nerve nucleus.As a starting point in the study of brainstem nuclei, this study explores the three-dimensional morphology and location of the thin bundles.According to the different advantages and disadvantages of different slice methods, two brain stem samples from adult Chinese males were selected to be made by paraffin section method.The brain stem specimen is divided into pieces, fixed the location point, marked in turn, made into wax block and sliced.There are many methods of brain nerve tissue staining. Tar violet staining is used in this study.Put the tar-stained film under a positive microscope, and take each one according to the location.The next step of image correction is to prepare for subsequent image segmentation.Image segmentation is a necessary process for extracting interesting information and computer 3D reconstruction.In this study, automatic segmentation and manual segmentation are combined to carry out image segmentation.There are many methods for automatic segmentation. In this study, Canny edge detection method is used for automatic segmentation of thin beam cores.Then the three-dimensional reconstruction of the thin beam nucleus begins.After comparing the advantages and disadvantages of the two methods, the surface reconstruction method for dense data field is selected to carry out 3D reconstruction of thin beam nucleus.There are some defects in the existing methods of measuring and statistics of three dimensional nuclear parameters. In this study, the shape averaging and measuring average three dimensional indexes are used to measure and count the thin beam nuclei.Through the above research process, we have obtained three results: 1. The three-dimensional model of thin beam nucleus can be observed at any angle, cut and analyzed with any orientation, and can be visualized and visualized in computer.The three-dimensional shape and position of the thin beam nucleus are shown vividly. 2. The data of the three-dimensional index of the thin beam nucleus are obtained, which indicates that the thin beam nucleus is a symmetrical irregular nucleus on both sides of the left and right.The right side is slightly larger than the left side: the right thin bundle nucleus is 8.659mm in length, 5.842mm in width, 4.897mm in height and 67.80mm3mm in volume.The length of the left thin nucleus is 8.411mm, the width is 4.250mm, and the height is 3.560mm. The volume 51.36mm3.3 has obtained a continuous, clear boundary of nuclei and a complete structure of the tomograms of the medulla oblongata of the brain stem.
【學位授予單位】：杭州師范大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R322

【參考文獻】

相關(guān)博士學位論文 前1條

1 崔高宇;基底節(jié)區(qū)定位解剖及可視化研究[D];第三軍醫(yī)大學;2003年

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本文編號：1729114