本文選題：microRNA　切入點(diǎn)：缺血再灌注　出處：《青島大學(xué)》2012年碩士論文

【摘要】：目的：檢測大鼠肺組織缺血再灌注損傷過程中后microRNA(miRNA)的表達(dá)變化,探討microRNA與肺組織缺血及再灌注損傷的相關(guān)性。 方法：采用改良Eppinger描述的大鼠肺缺血再灌注損傷動物模型,將25只大鼠隨機(jī)分為五組,分別為對照組、缺血組(1h)與缺血再灌注組(1h、3h、6h三個亞組)。應(yīng)用基因芯片技術(shù)(microarray)檢測對照組、缺血組與缺血再灌注組肺組織中microRNA表達(dá)水平。運(yùn)用stem-loop RT-qPCR法檢測芯片結(jié)果明顯變化的4個microRNA在對照組、缺血組(1h)與缺血再灌注組(1h、3h、6h三個亞組)五組中的表達(dá)水平。動態(tài)觀察大鼠肺缺血再灌注損傷急性期microRNA的變化。 結(jié)果：1.基因芯片技術(shù)(microarray)檢測正常組與缺血組肺組織中390個microRNA表達(dá)水平變化,與對照組相比,缺血肺組織中有4個miRNA表達(dá)上調(diào)兩倍及兩倍以上,21個miRNA表達(dá)下調(diào)兩倍及兩倍以上。 2.運(yùn)用stem-loop RT-qPCR檢測法檢測(?)miR-1、miR-18a、miR-31和miR-451在對照組與實(shí)驗(yàn)組的肺組織中的表達(dá)水平,發(fā)現(xiàn)在對照組、缺血組及再灌注組的各個時間點(diǎn)miR-18a表達(dá)先上調(diào)再下調(diào)；miR-31表達(dá)先下調(diào)再上調(diào)再下調(diào),在再灌注1h達(dá)到最低點(diǎn),再灌注3h表達(dá)水平最高；miR-451表達(dá)先上調(diào)再下調(diào),在再灌注3h達(dá)到最高點(diǎn)。 結(jié)論：大鼠肺組織急性缺血期,多個miRNA表達(dá)發(fā)生變化,提示miRNA參與肺缺血損傷過程。與調(diào)亡相關(guān)的miR-1、miR-31、miR-451在肺缺血再灌注過程中表達(dá)上調(diào)或下調(diào),提示miRNA參與肺缺血再灌注損傷的機(jī)制與細(xì)胞凋亡密切相關(guān)。與血管生成抑制相關(guān)的miR-18a在肺缺血再灌注過程中表達(dá)上調(diào)或下調(diào),提示miRNA參與肺缺血再灌注損傷的機(jī)制與血管生成也密切相關(guān)。
[Abstract]:Objective: to detect the expression of microRNA (miRNA) in lung tissue during ischemia-reperfusion injury in rats, and to explore the correlation between microRNA and lung ischemia-reperfusion injury.
Methods: the lung ischemia modified Eppinger described reperfusion injury of rat animal model, 25 rats were randomly divided into five groups, which were control group, ischemia group (1H) and ischemia reperfusion group (1H, 3h, 6h three groups). The application of gene chip technology (microarray) detection and control the expression level of microRNA group, ischemia group and ischemia reperfusion group in the lung tissue. Using stem-loop RT-qPCR method to detect the microarray results obvious change of 4 microRNA in the control group, ischemia group (1H) and ischemia reperfusion group (1H, 3h, 6h three groups) expression levels in the five groups. To observe the dynamic change lung ischemia reperfusion injury in rat acute microRNA.
Results: 1.. Gene chip technology (microarray) was used to detect 390 microRNA expression levels in lung tissue of normal group and ischemic group. Compared with control group, 4 miRNA expression increased two times and two times higher than that of control group, 21 miRNA expression was down two times and two times.
2. using stem-loop RT-qPCR to detect (?) miR-1, miR-18a, the expression level of miR-31 and miR-451 in the experimental group and the control group in the lung tissues, found in the control group, ischemia group and reperfusion group at each time point miR-18a was upregulated first and then down; the expression of miR-31 under the first tune and then raised down again, to the lowest point at 1h of reperfusion, 3h of reperfusion, the highest expression level; the expression of miR-451 increased firstly and then down, reached the highest point at 3h after reperfusion.
Conclusion: the lung tissue of rats with acute ischemia, multiple miRNA expression changes, suggesting that miRNA involved in lung ischemia. And the apoptosis related miR-1, miR-31, expression of miR-451 in lung ischemia reperfusion process, suggesting that miRNA in lung ischemia reperfusion injury and the mechanism of apoptosis is closely related with. Inhibition of angiogenesis related miR-18a expression in lung ischemia reperfusion process, suggesting that miRNA in lung ischemia reperfusion injury and mechanism of vascular formation are closely related.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R363

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 滕建曦;劉國慶;孔亮亮;黎慶鋒;孔連寶;;微小RNA29調(diào)節(jié)小鼠肝臟缺血再灌注損傷的研究[J];中華臨床醫(yī)師雜志(電子版);2013年09期

2 石彥超;施明;劉振文;王福生;;microRNA在肝移植研究中的進(jìn)展[J];肝臟;2013年10期

相關(guān)博士學(xué)位論文 前3條

1 劉芬;缺氧誘導(dǎo)miRNA調(diào)控腎缺血后血管新生的分子機(jī)制[D];南昌大學(xué);2011年

2 張毅;腹部創(chuàng)傷應(yīng)激后血清microRNA表達(dá)改變及其意義[D];第四軍醫(yī)大學(xué);2011年

3 陳軍瑩;鱗狀上皮細(xì)胞癌抗原和微小RNA作為宮頸鱗癌患者淋巴結(jié)轉(zhuǎn)移血清標(biāo)記物的研究[D];廣西醫(yī)科大學(xué);2013年

相關(guān)碩士學(xué)位論文 前5條

1 曲秋月;肝病患者血漿中相關(guān)miRNA和mRNA的檢測分析[D];中國人民解放軍軍事醫(yī)學(xué)科學(xué)院;2011年

2 鄧君;腎缺血再灌注損傷后miR-210及其靶基因的變化[D];南昌大學(xué);2010年

3 劉國巖;肝臟缺血再灌注損傷的評估及防護(hù)[D];安徽醫(yī)科大學(xué);2013年

4 黃峰;風(fēng)濕性心臟病伴房顫患者心房肌microRNA差異表達(dá)的觀察[D];中南大學(xué);2013年

5 李俊平;MicroRNA-24及其靶基因GATA-2在腎缺血再灌注損傷的表達(dá)變化[D];華中科技大學(xué);2013年

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本文編號：1708895