本文選題：microRNA　切入點：缺血再灌注　出處：《青島大學》2012年碩士論文

【摘要】：目的：檢測大鼠肺組織缺血再灌注損傷過程中后microRNA(miRNA)的表達變化,探討microRNA與肺組織缺血及再灌注損傷的相關性。 方法：采用改良Eppinger描述的大鼠肺缺血再灌注損傷動物模型,將25只大鼠隨機分為五組,分別為對照組、缺血組(1h)與缺血再灌注組(1h、3h、6h三個亞組)。應用基因芯片技術(microarray)檢測對照組、缺血組與缺血再灌注組肺組織中microRNA表達水平。運用stem-loop RT-qPCR法檢測芯片結(jié)果明顯變化的4個microRNA在對照組、缺血組(1h)與缺血再灌注組(1h、3h、6h三個亞組)五組中的表達水平。動態(tài)觀察大鼠肺缺血再灌注損傷急性期microRNA的變化。 結(jié)果：1.基因芯片技術(microarray)檢測正常組與缺血組肺組織中390個microRNA表達水平變化,與對照組相比,缺血肺組織中有4個miRNA表達上調(diào)兩倍及兩倍以上,21個miRNA表達下調(diào)兩倍及兩倍以上。 2.運用stem-loop RT-qPCR檢測法檢測(?)miR-1、miR-18a、miR-31和miR-451在對照組與實驗組的肺組織中的表達水平,發(fā)現(xiàn)在對照組、缺血組及再灌注組的各個時間點miR-18a表達先上調(diào)再下調(diào)；miR-31表達先下調(diào)再上調(diào)再下調(diào),在再灌注1h達到最低點,再灌注3h表達水平最高；miR-451表達先上調(diào)再下調(diào),在再灌注3h達到最高點。 結(jié)論：大鼠肺組織急性缺血期,多個miRNA表達發(fā)生變化,提示miRNA參與肺缺血損傷過程。與調(diào)亡相關的miR-1、miR-31、miR-451在肺缺血再灌注過程中表達上調(diào)或下調(diào),提示miRNA參與肺缺血再灌注損傷的機制與細胞凋亡密切相關。與血管生成抑制相關的miR-18a在肺缺血再灌注過程中表達上調(diào)或下調(diào),提示miRNA參與肺缺血再灌注損傷的機制與血管生成也密切相關。
[Abstract]:Objective: to detect the expression of microRNA (miRNA) in lung tissue during ischemia-reperfusion injury in rats, and to explore the correlation between microRNA and lung ischemia-reperfusion injury.
Methods: the lung ischemia modified Eppinger described reperfusion injury of rat animal model, 25 rats were randomly divided into five groups, which were control group, ischemia group (1H) and ischemia reperfusion group (1H, 3h, 6h three groups). The application of gene chip technology (microarray) detection and control the expression level of microRNA group, ischemia group and ischemia reperfusion group in the lung tissue. Using stem-loop RT-qPCR method to detect the microarray results obvious change of 4 microRNA in the control group, ischemia group (1H) and ischemia reperfusion group (1H, 3h, 6h three groups) expression levels in the five groups. To observe the dynamic change lung ischemia reperfusion injury in rat acute microRNA.
Results: 1.. Gene chip technology (microarray) was used to detect 390 microRNA expression levels in lung tissue of normal group and ischemic group. Compared with control group, 4 miRNA expression increased two times and two times higher than that of control group, 21 miRNA expression was down two times and two times.
2. using stem-loop RT-qPCR to detect (?) miR-1, miR-18a, the expression level of miR-31 and miR-451 in the experimental group and the control group in the lung tissues, found in the control group, ischemia group and reperfusion group at each time point miR-18a was upregulated first and then down; the expression of miR-31 under the first tune and then raised down again, to the lowest point at 1h of reperfusion, 3h of reperfusion, the highest expression level; the expression of miR-451 increased firstly and then down, reached the highest point at 3h after reperfusion.
Conclusion: the lung tissue of rats with acute ischemia, multiple miRNA expression changes, suggesting that miRNA involved in lung ischemia. And the apoptosis related miR-1, miR-31, expression of miR-451 in lung ischemia reperfusion process, suggesting that miRNA in lung ischemia reperfusion injury and the mechanism of apoptosis is closely related with. Inhibition of angiogenesis related miR-18a expression in lung ischemia reperfusion process, suggesting that miRNA in lung ischemia reperfusion injury and mechanism of vascular formation are closely related.

【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R363

【共引文獻】

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本文編號：1708895