本文選題：RNF　切入點(diǎn)：免疫　出處：《軍事醫(yī)學(xué)》2017年01期

【摘要】：目的研究環(huán)指蛋白34(RING finger protein 34,RNF34)對(duì)天然免疫的調(diào)控。方法利用重組PCR方法構(gòu)建pcDNA3-Flag-RNF34、pcDNA3-Flag-RNF34-ΔFYVE、pcDNA3-Flag-RNF34-ΔCID和pcDNA3-Flag-RNF34-ΔRING質(zhì)粒,并在HEK293T細(xì)胞中瞬時(shí)表達(dá);利用雙熒光素酶報(bào)告基因技術(shù)檢測(cè)RNF34及其3種突變體對(duì)仙臺(tái)病毒(SeV)和N-RIG-Ⅰ激活NF-κB和IFN-β轉(zhuǎn)錄活性的影響。結(jié)果成功構(gòu)建了Flag-RNF34及其3種結(jié)構(gòu)域缺失突變體的真核表達(dá)質(zhì)粒;發(fā)現(xiàn)在SeV刺激下,RNF34對(duì)NF-κB和IFN-β轉(zhuǎn)錄活性有明顯抑制作用,RNF34-ΔFYVE、RNF34-ΔCID和RNF34-ΔRING與RNF34相比此種抑制作用減弱。同時(shí)發(fā)現(xiàn)對(duì)于N-RIG-Ⅰ激活的NF-κB和IFN-β活性,RNF34及其3種結(jié)構(gòu)域缺失突變體也有相似的抑制作用。結(jié)論 RNF34通過負(fù)調(diào)控RIG-Ⅰ-MAVS信號(hào)通路調(diào)控天然免疫。
[Abstract]:Objective to study the regulation of innate immunity by cyclic finger protein 34(RING finger protein 34RNF34. methods pcDNA3-Flag-RNF34- 螖 FYVEV pcDNA3-Flag-RNF34- 螖 CID and pcDNA3-Flag-RNF34- 螖 RING were constructed by recombinant PCR method and expressed in HEK293T cells. Double luciferase reporter gene technique was used to detect the effects of RNF34 and its three mutants on the transcriptional activities of Sendai virus (Sendai virus) and N-RIG- 鈪，

本文編號(hào)：1695275