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  本文選題：電針　切入點：犬　出處：《西南大學(xué)》2011年碩士論文

【摘要】：慢性胃炎是消化系統(tǒng)的常見病、多發(fā)病,是胃粘膜在各種致病因素的作用下發(fā)生的慢性炎癥病變或萎縮性病變。隨著中醫(yī)學(xué)對本病病因病機認識的深入,運用針灸治療慢性胃炎積累了豐富的經(jīng)驗。本實驗通過采用水楊酸鈉灌胃,并以自由飲用氨水和饑飽失常相結(jié)合的綜合造模法建立犬的脾胃虛寒型慢性胃炎模型。造模成功后,除模型對照組和正常對照組外,電針穴位足三里、內(nèi)關(guān)、胃俞進行治療4周。治療期間通過觀察犬體重變化、臨床癥狀和病理學(xué)變化、胃酸PH值及胃蛋白酶活性,對各組前肢頭靜脈采血檢測部分血常規(guī)及血生化指標,判定電針治療犬脾胃虛寒型慢性胃炎的療效。 健康成年雜種犬15只,體重10-15kg,隨機分為3組,即正常對照組、模型對照組,電針治療組,每組5只。除正常對照組只給生理鹽水灌胃外,其余各組均采用水楊酸鈉灌胃,并自由飲用氨水,饑飽失常法建立脾胃虛寒型慢性胃炎模型,造模2個月,結(jié)果表明,模型犬消瘦,皮毛粗糙,食量減少,倦怠無力,常有稀便或黑便。模型組犬較正常對照組體重有顯著性差異(P0.05),模型對照組與正常對照組比較,白細胞(WBC)、紅細胞(RBC)、紅細胞壓積(HCT)、平均紅細胞體積(MCV)、淋巴細胞(LYM)下降顯著(P0.05)；谷草轉(zhuǎn)氨酶(AST)、谷丙轉(zhuǎn)氨酶(ALT)、堿式磷酸酶(ALP)升高顯著(P0.05)；總蛋白(TP)、血清葡萄糖(GLU)下降顯著(P0.05)；胃液PH值明顯高于正常對照組(P0.01),胃蛋白酶活力顯著下降(P0.01)。 犬脾胃虛寒型慢性胃炎模型建立后,除模型對照組和正常對照組外,電針穴位足三里、內(nèi)關(guān)、胃俞進行治療4周。結(jié)果表明,治療組臨床癥狀得到明顯改善,與模型對照組比較WBC、RBC、HCT、LYM顯著升高(P0.05);AST、ALT、ALP顯著下降(P0.05);TP、GLU顯著升高(P0.05)；胃液PH值顯著下降(P0.01),胃蛋白酶活力顯著升高(P0.01)。 本實驗建立的犬脾胃虛寒慢性胃炎模型,符合慢性胃炎的主要臨床表現(xiàn)及病理學(xué)特點,且模型維持時間長,有利于進一步進行基礎(chǔ)及臨床研究。試驗所選取的穴位及治療辦法可以明顯緩解慢性胃炎癥狀,為獸醫(yī)臨床治療犬脾胃虛寒慢性胃炎提供了實驗依據(jù)。
[Abstract]:Chronic gastritis is a common disease of digestive system. It is a kind of chronic inflammation or atrophic lesion of gastric mucosa under the action of various pathogenic factors. This experiment has accumulated rich experience in the treatment of chronic gastritis by acupuncture and moxibustion. The model of chronic gastritis of deficiency of spleen and stomach in dogs was established by using sodium salicylate, combined with free drinking ammonia water and hunger and satiety disorder. In addition to the model control group and the normal control group, electroacupuncture at Zusanli, Neiguan and Weishu were treated for 4 weeks. During the treatment, the changes of body weight, clinical symptoms and pathology, gastric acid PH and pepsin activity were observed. To detect part of blood routine and blood biochemical index of forelimb head vein in each group, to evaluate the curative effect of electroacupuncture on chronic gastritis with deficiency of spleen and stomach. Fifteen healthy adult mongrel dogs, weighing 10-15 kg, were randomly divided into three groups: normal control group, model control group and electroacupuncture treatment group. The model of chronic gastritis with deficiency of spleen and stomach was established by the method of free drinking ammonia water and hunger and satiety. The results showed that the model dogs were thin, rough fur, reduced food intake and weak burnout. There was a significant difference in body weight between the model group and the normal control group (P 0.05), and the comparison between the model control group and the normal control group. WBC, RBC, HCT, mean RBC volume and LYM decreased significantly (P 0.05), glutamic oxalacetic transaminase (AST), alanine aminotransferase (alt), alkaline phosphatase (ALP) increased significantly (P0.05a), total protein (TPN) and serum glucose (GLU) decreased significantly (P 0.05). The PH value of gastric juice was significantly higher than that of the normal control group (P 0.01), and the pepsin activity decreased significantly (P 0.01). After the establishment of the model of chronic gastritis with deficiency of spleen and stomach, electroacupuncture treatment of Zusanli, Neiguan and Weishu at acupoints was carried out for 4 weeks in addition to the model control group and the normal control group. The results showed that the clinical symptoms of the treatment group were obviously improved. Compared with the model control group, the level of HCTlYM in WBC- RBC was significantly higher than that in the model control group, and the ALP of AST was decreased significantly, the TPGLU of P0.05 was significantly increased, the pH of gastric juice was decreased significantly (P0.01), the activity of pepsin was increased significantly (P0.01). The model of chronic gastritis with deficiency of spleen and stomach was established in this experiment, which was consistent with the main clinical manifestations and pathological characteristics of chronic gastritis, and the model lasted for a long time. The points and treatment methods selected in the experiment can obviously alleviate the symptoms of chronic gastritis and provide experimental basis for the treatment of chronic gastritis in dogs with deficiency of spleen and stomach.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332;R245

【參考文獻】

相關(guān)期刊論文 前10條

1 許冠蓀;王振玖;朱舜麗;陳全珠;焦?jié)?張道芹;;一氧化氮參與針刺對大鼠胃粘膜損傷的保護作用[J];安徽中醫(yī)學(xué)院學(xué)報;1996年03期

2 黃濤;針灸治療膽汁反流性胃炎的臨床研究[J];中國醫(yī)藥學(xué)報;2000年01期

3 趙保民,黃裕新,趙寧俠,劉梅,尚磊,黃梅,王慶莉;電針調(diào)控大鼠胃酸分泌的機制[J];第四軍醫(yī)大學(xué)學(xué)報;2001年09期

4 繆勤;張匯東;溫海;;犬胃病的臨床診斷與治療[J];警犬;2003年01期

5 張淑芹,趙林山,鄭繼奎,楊永良,郭立波;慢性萎縮性胃炎動物模型的復(fù)制[J];哈爾濱師范大學(xué)自然科學(xué)學(xué)報;2001年06期

6 姚春;李娜;;慢性胃炎動物模型復(fù)制的實驗研究進展[J];遼寧中醫(yī)藥大學(xué)學(xué)報;2010年01期

7 何慕倫;楊文輝;莊禮興;司徒鈴;陳芝喜;賈可亮;李志強;陳津巖;;不同針刺手法對脾虛模型兔血清胃動素和胃泌素含量的影響[J];廣州中醫(yī)學(xué)院學(xué)報;1991年01期

8 李順民;鄧鐵濤;;重癥肌無力脾虛證唾液淀粉酶活性及D—木糖排泄率分析[J];廣州中醫(yī)學(xué)院學(xué)報;1991年04期

9 張連城,張升堂,孫彥榮;辰時針足三里治療慢性胃炎[J];四川中醫(yī);1991年02期

10 王莉;針灸治療慢性胃炎90例臨床療效觀察[J];四川中醫(yī);1997年09期

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