本文選題：神經(jīng)干細(xì)胞　切入點：人工腦脊液　出處：《華中科技大學(xué)》2011年碩士論文

【摘要】：目的 探討體外培養(yǎng)的神經(jīng)干細(xì)胞(neural stem cells,NSCs)能否在人工腦脊液(artificial cerebrospinal fluid, ACSF)中生長,從而為進(jìn)一步的細(xì)胞移植治療神經(jīng)系統(tǒng)疾病奠定基礎(chǔ)。 方法 體外培養(yǎng)NSCs在傳2代時,分別接種到ACSF中和常規(guī)神經(jīng)元培養(yǎng)液中培養(yǎng),觀察、比較NSCs在ACSF中及常規(guī)神經(jīng)元培養(yǎng)液的生長情況,并于培養(yǎng)第3天時進(jìn)行Tubulin、GFAP免疫熒光染色。 結(jié)果 在此實驗條件下,NSCs在ACSF中可以存活、生長,且Tubulin、GFAP免疫熒光染色均為陽性。但細(xì)胞形態(tài)較在常規(guī)神經(jīng)元培養(yǎng)液中培養(yǎng)的細(xì)胞有差別,在ACSF中分化的細(xì)胞突起細(xì)長,胞體欠飽和。 結(jié)論 在此實驗條件下,NSCs在ACSF中可以存活、分化。 目的 探討體外培養(yǎng)的神經(jīng)干細(xì)胞(neural stem cells, NSCs)在人工腦脊液(artificial cerebrospinal fluid, ACSF)中的最佳移植時間,為細(xì)胞移植治療神經(jīng)系統(tǒng)疾病奠定基礎(chǔ)。 方法 體外培養(yǎng)NSCs在傳2代時進(jìn)行誘導(dǎo)分化,分別在誘導(dǎo)分化期開始第1、2、3、4、5、6天時收獲細(xì)胞,重新接種于ACSF中再培養(yǎng)3 d,檢測NSCs在ACSF中分化為神經(jīng)元的百分率,MTT試驗檢測細(xì)胞相對生長活力,初步探討NSCs在ACSF中的最佳移植時間。 結(jié)果 在此實驗條件下,NSCs在ACSF中可以存活并且能夠生長,經(jīng)統(tǒng)計學(xué)分析顯示,經(jīng)誘導(dǎo)分化4 d的NSCs,接種于ACSF后分化為神經(jīng)元的百分率及生長活力顯著高于其他時間點(P0.05)。 結(jié)論 在此實驗條件下,NSCs在體外誘導(dǎo)分化后第4天時,可能是最佳移植時間。
[Abstract]:Purpose. To investigate whether neural stem cells can grow in artificial cerebrospinal fluid (cerebrospinal) in vitro, so as to lay a foundation for further cell transplantation in the treatment of nervous system diseases. Method. In vitro culture of NSCs was inoculated into ACSF and conventional neuronal culture medium respectively at the second generation. The growth of NSCs in ACSF and conventional neuronal culture medium was compared, and the immunofluorescence staining of NSCs was performed on the 3rd day of culture. Results. Under these experimental conditions, ACSF could survive and grow, and tubal tubulinine GFAP immunofluorescence staining was positive, but the cell morphology was different from that in normal neuron culture medium. The cells differentiated in ACSF had slender processes and undersaturated cell bodies. Conclusion. Under these experimental conditions, NSCs can survive and differentiate in ACSF. Purpose. To investigate the optimal transplantation time of neural stem cells (NSCs) in artificial cerebrospinal fluid (cerebrospinal) in vitro, and to lay a foundation for the treatment of nervous system diseases by cell transplantation. Method. NSCs was cultured in vitro to induce differentiation at the second generation, and the cells were harvested at the beginning of the first day of differentiation at the beginning of the first day of differentiation. The percentage of NSCs differentiated into neurons in ACSF was detected by reseeding in ACSF for 3 days. The relative growth activity of cells was detected by MTT assay. The optimal transplantation time of NSCs in ACSF was preliminarily discussed. Results. Under these experimental conditions, NSCs could survive and grow in ACSF. Statistical analysis showed that the percentage and growth activity of NSCs differentiated into neurons after 4 days of differentiation after inoculation with ACSF were significantly higher than those at other time points (P0.05). Conclusion. Under these conditions, NSCs may be the best transplantation time on the 4th day after induction and differentiation in vitro.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R329

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