本文選題：TD1　切入點(diǎn)：透皮給藥　出處：《中國(guó)科學(xué)技術(shù)大學(xué)》2011年碩士論文

【摘要】：透皮給藥相對(duì)于傳統(tǒng)的口服與皮下注射給藥方式而言,其具有方便性、可持續(xù)性和可控制性強(qiáng)等優(yōu)點(diǎn)。近年來(lái),透皮給藥的研究與應(yīng)用為治療疾病的給藥方式提供了新的途徑。 TD1是一個(gè)具有11個(gè)氨基酸的短肽(ACSSSPSKHCG),其能夠通過(guò)簡(jiǎn)單混合涂抹給藥的方式促進(jìn)胰島素等蛋白大分子藥物透過(guò)皮膚到達(dá)動(dòng)物體血液循環(huán)中而發(fā)揮治療作用。本文中我們利用分子生物學(xué)方法使TD1通過(guò)GGGGS共價(jià)連接至EGF的N端,得到了TD1-EGF融合蛋白。我們發(fā)現(xiàn)TD1-EGF與普通的EGF相比,具有類(lèi)似的促細(xì)胞生長(zhǎng)、遷移與刺激ERK1/2磷酸化激活的細(xì)胞活性,且比普通的EGF更容易透過(guò)完整的動(dòng)物皮膚。TD1-EGF的促透作用具有明顯的時(shí)間與濃度依賴性,并且這種顯著的促透效果需要正常的體溫條件。這可能是TD1與皮膚間存在相互作用而并非與藥物作用的結(jié)果。 通過(guò)本文對(duì)TD1-EGF的細(xì)胞活性與透皮活性的研究,為T(mén)D1-EGF在經(jīng)皮給藥、損傷修復(fù)以及醫(yī)療美容等領(lǐng)域的應(yīng)用提供了理論基礎(chǔ)。
[Abstract]:Compared with the traditional oral and subcutaneous administration, transdermal administration has the advantages of convenience, sustainability and controllability. In recent years, the research and application of transdermal drug delivery have provided a new way for the treatment of diseases. TD1 is a short peptide with 11 amino acids, ACSSSPSKHCG, which can promote insulin and other protein macromolecular drugs through the skin to reach the blood circulation of animal body and play a therapeutic role. We used molecular biology to coconnect TD1 to the N-terminal of EGF via GGGGS. TD1-EGF fusion protein was obtained. We found that TD1-EGF has similar cellular activity to promote cell growth, migrate and stimulate phosphorylation of ERK1/2, as compared with normal EGF. Moreover, the permeation effect of TD1-EGF through intact animal skin was more obvious in a time-and concentration-dependent manner than that of normal EGF. And this remarkable penetration effect requires normal body temperature conditions, which may be the result of interaction between TD1 and skin rather than drugs. The study on the cellular and transdermal activity of TD1-EGF provides a theoretical basis for the application of TD1-EGF in the field of transdermal drug delivery, injury repair, medical beauty and so on.
【學(xué)位授予單位】：中國(guó)科學(xué)技術(shù)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R346

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