本文選題：細菌/診斷　切入點：真菌/診斷　出處：《浙江大學(xué)》2012年碩士論文

【摘要】：目的： 通過檢測部分常見的細菌與真菌菌株代謝產(chǎn)生的揮發(fā)性有機物(volatile organic compounds VOCs),篩選出各菌株產(chǎn)生的內(nèi)源性VOCs,為呼吸氣體在肺部感染性疾病的診斷提供實驗依據(jù)。 方法： 利用固相微萃取聯(lián)合氣相色譜質(zhì)譜儀連用技術(shù)(solid-phase microextraction and gas chromatography mass spectroscopy,SPME/GC-MS),檢測體外培養(yǎng)菌株混懸液(大腸桿菌、銅綠假單胞菌、肺炎克雷伯桿菌、金黃色葡萄球菌、腸球菌、白色念珠菌、光滑念珠菌)頂空氣體中的VOCs,從中篩選出各種病原菌的特征性VOCs。 結(jié)果： 試驗中大腸桿菌、銅綠假單胞菌、肺炎克雷伯桿菌及白假絲酵母菌可檢測到特征性VOCs,大腸桿菌、銅綠假單胞菌及肺炎克雷伯桿菌中可分別檢測到特征性吲哚、十一烷醇及丁基羥基甲苯,白假絲酵母菌可檢測到C15H260和C17H2802；大腸桿菌與銅綠假單胞菌的混合菌懸液可檢測到吲哚及十一烷醇,與各自培養(yǎng)檢測到的VOC相同,相互之間無影響；細胞株與微生物混合培養(yǎng),結(jié)果與單純微生物培養(yǎng)的VOCs相同,提示細胞株不影響微生物代謝；白假絲酵母菌與光滑念珠菌的特征性VOCs不同,提示通過檢測細菌與真菌特征性VOCs不僅能鑒別菌屬,且能鑒別相近的菌種。 結(jié)論： 部分體外培養(yǎng)的細菌與真菌可通過代謝產(chǎn)生特征性VOCs,兩種細菌混合生長仍可產(chǎn)生各自的特征性VOCs,A549細胞株不影響細菌特征性VOCs;SPEM/GC-MS是一種可用于檢測部分細菌和真菌特征性VOCs的無創(chuàng)快速有效的方法,且可鑒別相近的菌種。
[Abstract]:Objective:. By detecting volatile organic compounds VOCs produced by metabolism of some common bacteria and fungal strains, the endogenous VOCs produced by each strain were screened, which provided experimental basis for the diagnosis of respiratory gas in pulmonary infectious diseases. Methods:. Solid phase microextraction and gas chromatography mass spectroscopy SPME / GC MS / MS was used to detect the suspension (E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus) in vitro by solid phase microextraction (SPME) combined with gas chromatographic mass spectrometry (GC). The characteristic VOCs in the headspace gas of Candida albicans were screened out. Results:. E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Candida albicans could be detected in E. coli, E. coli, Pseudomonas aeruginosa and Klebsiella pneumoniae respectively. C15H260 and C17H2802 could be detected in undecanol and Ding Ji hydroxy toluene, C15H260 and C17H2802 could be detected in Escherichia coli and Pseudomonas aeruginosa suspension, indole and undecanol could be detected in mixed suspension of Escherichia coli and Pseudomonas aeruginosa, which were the same as VOC detected in culture and had no effect on each other. The results of mixed culture of cell line and microorganism were the same as that of VOCs cultured by pure microorganism, suggesting that the cell strain had no effect on microbial metabolism, and the characteristic VOCs of Candida albicans and Candida glabra were different. It is suggested that the characteristic VOCs of bacteria and fungi can not only identify the genus of bacteria, but also identify the similar species. Conclusion:. Some of the bacteria and fungi cultured in vitro can produce characteristic VOCs by metabolism, and the mixed growth of the two bacteria can still produce their own characteristic VOCsSU A549 cell lines, which can be used for the detection of some bacteria and fungi. A noninvasive, fast and effective method for characteristic VOCs, And the similar strains can be identified.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R378

【參考文獻】

相關(guān)期刊論文 前3條

1 蹇在金,廖紀(jì)南;老年人肺炎的病因與臨床[J];中華老年醫(yī)學(xué)雜志;2004年10期

2 吳巖;;老年肺炎102例臨床分析[J];中國社區(qū)醫(yī)師(醫(yī)學(xué)專業(yè));2010年35期

3 胡燕婕;邱園華;陳恩國;應(yīng)可凈;於錦;王平;;有機性揮發(fā)物在肺癌組織和癌細胞株中的檢測和分析[J];浙江大學(xué)學(xué)報(醫(yī)學(xué)版);2010年03期

相關(guān)碩士學(xué)位論文 前1條

1 陶孝芬;2007～2009年兒童肺炎支原體肺炎流行病學(xué)調(diào)查[D];浙江大學(xué);2010年

，

本文編號：1659816