本文選題：鉤端螺旋體　切入點(diǎn)：宿主細(xì)胞　出處：《浙江大學(xué)》2012年碩士論文

【摘要】：目的：了解人和小鼠鉤端螺旋體(簡(jiǎn)稱鉤體)主要宿主細(xì)胞上整合素和鈣通道表達(dá)情況及其差異。 方法：采用免疫熒光和Western Blot法,檢測(cè)人單核細(xì)胞株THP-1、小鼠單核-巨噬樣細(xì)胞株J774A.1、人臍靜脈內(nèi)皮細(xì)胞株HUVEC.小鼠血管內(nèi)皮細(xì)胞株EOMA.人肝細(xì)胞株L-02、小鼠肝細(xì)胞株Hepa1-6、人腎小管上皮細(xì)胞株HEK-293、小鼠腎小球膜上皮細(xì)胞株SV40-MES13、小鼠腎成纖維細(xì)胞株NIH/3T3、人及小鼠血小板的β1、β2、β3類整合素分子以及CaV3.1、CaV3.2、CaV3.3、CaV2.3電壓門控型鈣通道蛋白和P2X1、P2X2、P2X3、P2X4、P2X5、P2X6、P2X7受體門控型鈣通道蛋白的分布差異。 結(jié)果：整合素β1分子表達(dá)于J774A.1、THP-1、HUVEC、EOMA、L-02、Hepa1-6、 HEK-239細(xì)胞以及人和小鼠血小板膜表面,β2分子表達(dá)于J774A.1、和THP-1細(xì)胞膜表面,p3分子表達(dá)于J774A.1、THP-1、HUVEC、EOMA、Hepa1-6、HEK-239、 SV40-MES13和NIH-3T3細(xì)胞膜表面。ATP依賴性受體門控型鈣通道蛋白P2X1表達(dá)于人和小鼠血小板膜表面,P2X5表達(dá)于J774A.1、THP-1、L-02、Hepa1-6、HEK-239和HUVEC細(xì)胞膜表面,但未檢測(cè)出上述細(xì)胞或血小板表達(dá)其它鈣通道蛋白。 結(jié)論：不同種類整合素分子和鈣通道蛋白在鉤體主要宿主細(xì)胞膜上表達(dá)明顯不同,這可能與鉤體對(duì)不同組織細(xì)胞致病性差異有關(guān)。
[Abstract]:Aim: to investigate the expression of integrin and calcium channel in human and mouse Leptospira leptospirosis (Leptospira) host cells and their differences. Methods: immunofluorescence and Western Blot assay were used. Human monocyte cell line THP-1, mouse monocyte-macrophage-like cell line J774A.1, human umbilical vein endothelial cell line HUVEC.Mouse vascular endothelial cell line EOMA.Human hepatocyte cell line L-02, mouse hepatocyte line Hepa1-6, human renal tubular epithelial cell line HEK-293and mouse glomerular membrane. The distribution of 尾 _ 1, 尾 _ 2, 尾 _ 3 integrin molecules in human and mouse platelets, CaV3.1, CaV3.2, CaV2.3 voltage-gated calcium channel protein and P2X1, P2X2P2X2P2X5, P2X5, P2X6, P2X6, P2X7 receptor gated calcium channel protein were different in epithelial cell line SV40-MES13, mouse renal fibroblast cell line NIH / 3T3, human platelets and mouse platelets. Results: integrin 尾 1 was expressed on the surface of human and mouse platelets in HEK-239 cells and human and mouse platelets, and on J774A.1THP-1HUVEC-EOMAL-02Hepa1-6, and on J774A.1THP-1HUVEC-EMA-6HHEK-239. SV40-MES13 and NIH-3T3 cell membrane surface. ATP-dependent receptor-gated calcium channels. The protein P2X1 was expressed on the surface of human and mouse platelets membrane, and expressed on the surface of J774A.1THP-1 / L-02Hep A1-6 HEK-239 and HUVEC cell membrane. However, no expression of other calcium channel proteins was detected in the above cells or platelets. Conclusion: the expression of integrin molecules and calcium channel proteins in Leptospira host cell membrane is significantly different, which may be related to the pathogenicity of Leptospira to different tissues and cells.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R377.5

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本文編號(hào)：1658221