本文選題：組織胞漿菌　切入點(diǎn)：環(huán)介導(dǎo)等溫?cái)U(kuò)增技術(shù)　出處：《中國(guó)真菌學(xué)雜志》2016年02期

【摘要】：目的研發(fā)一種基于環(huán)介導(dǎo)等溫?cái)U(kuò)增(1oop-mediated isothermal amplification,LAMP)技術(shù)原理的組織胞漿菌感染的分子診斷技術(shù)。方法以組織胞漿菌為研究對(duì)象,針對(duì)其種特異性的M抗原基因序列,設(shè)計(jì)并篩選數(shù)套基于LAMP技術(shù)的特異性引物,同時(shí)優(yōu)化其反應(yīng)條件,建立一種針對(duì)組織胞漿菌感染的快速分子鑒定技術(shù)。結(jié)果該LAMP技術(shù)在65℃、90min反應(yīng)條件下,對(duì)組織胞漿菌基因組DNA的檢測(cè)敏感度達(dá)到5.3pg/反應(yīng)(即5.3×10-12 g,約160個(gè)基因組DNA拷貝),且與其相近種屬真菌無交叉反應(yīng)。結(jié)論我們研發(fā)的這種基于LAMP技術(shù)原理的組織胞漿菌分子診斷技術(shù)具有操作簡(jiǎn)便、特異性及敏感性高等優(yōu)點(diǎn),具有臨床推廣應(yīng)用的潛力。
[Abstract]:Objective to develop a molecular diagnostic technique based on the principle of cycle-mediated isothermal amplification of isothermal amplificationlampp. Methods the specific M antigen gene sequence of histiocytoplasmic bacteria was studied. Several sets of specific primers based on LAMP technique were designed and screened. At the same time, the reaction conditions were optimized, and a rapid molecular identification technique for histoplasmic bacteria infection was established. Results the LAMP technique was used at 65 鈩，

本文編號(hào)：1657607