本文選題：腦梗死　切入點(diǎn)：內(nèi)源性神經(jīng)干細(xì)胞　出處：《中國(guó)中西醫(yī)結(jié)合雜志》2017年02期 　論文類型：期刊論文

【摘要】：目的觀察電針對(duì)大腦中動(dòng)脈閉塞(middle cerebral artery occlusion,MCAO)后缺血再灌注(ischemia-reperfusion,I/R)不同時(shí)間點(diǎn)大鼠海馬區(qū)內(nèi)源性神經(jīng)干細(xì)胞(endogenous neural stem cells,e NSCs)表達(dá)的影響,探討電針對(duì)急性腦梗死(acute cerebral infarction,ACI)遠(yuǎn)隔損害的可能機(jī)制。方法雄性SPF級(jí)SD大鼠用線栓法制作MCAO模型,采用隨機(jī)數(shù)字表法分為假手術(shù)組、腦梗死組、電針組,每組30只;假手術(shù)組僅做手術(shù)創(chuàng)傷,腦梗死組僅作MCAO缺血再灌注處理;電針組選取"百會(huì)"和"大椎"行電針治療,每天1次,每次30 min;治療后第1、7、14天分別評(píng)定神經(jīng)功能缺損程度,同時(shí)在各組隨機(jī)選取6只大鼠處死,分離出缺血側(cè)海馬組織,采用免疫熒光法檢測(cè)海馬組織e NSCs增殖與分化表達(dá)。結(jié)果 (1)大鼠神經(jīng)功能缺損評(píng)分方面:與假手術(shù)組比較,腦梗死組在第1、7、14天神經(jīng)功能缺損評(píng)分增高(P0.05);與腦梗死組比較,電針組在第1、7、14天神經(jīng)功能缺損評(píng)分降低(P0.05)。(2)Brd U陽(yáng)性細(xì)胞表達(dá):與假手術(shù)比較,腦梗死組缺血側(cè)海馬CA1、CA3、DG處Brd U陽(yáng)性細(xì)胞數(shù)在第1、7、14天均增多(P0.05);與腦梗死組比較,電針組Brd U陽(yáng)性細(xì)胞數(shù)均增多(P0.05)。(3)Nestin陽(yáng)性細(xì)胞表達(dá):與假手術(shù)組比較,腦梗死組海馬齒狀回Nestin陽(yáng)性細(xì)胞數(shù)在第1、7、14天均增多(P0.05);與腦梗死組比較,電針組Nestin陽(yáng)性表達(dá)增加,但僅第7天差異明顯(P0.05)。(4)DCX陽(yáng)性細(xì)胞表達(dá):與假手術(shù)組比較,腦梗死組第1、7天DCX陽(yáng)性細(xì)胞增多(P0.05);與腦梗死組比較,電針組第7、14天DCX陽(yáng)性細(xì)胞增多(P0.05)。(5)Neu N陽(yáng)性細(xì)胞表達(dá):與假手術(shù)組比較,腦梗死組海馬齒狀回Neu N陽(yáng)性細(xì)胞表達(dá)在第1、7、14天均增加,但僅第14天時(shí)差異明顯(P0.05);與腦梗死組比較,電針組Neu N陽(yáng)性細(xì)胞表達(dá)增多,但僅第1、14天時(shí)差異明顯(P0.05)。(6)GFAP陽(yáng)性細(xì)胞表達(dá):與假手術(shù)組比較,腦梗死組缺血側(cè)海馬齒狀回GFAP陽(yáng)性細(xì)胞表達(dá)在第1、7、14天均明顯增多(P0.05);與腦梗死組比較,電針組GFAP陽(yáng)性細(xì)胞表達(dá)增多不顯著,僅第14天時(shí)差異明顯(P0.05)。結(jié)論大鼠腦梗死后海馬區(qū)存在e NSCs增殖與分化,電針治療可促進(jìn)腦梗死后神經(jīng)缺損功能恢復(fù),其機(jī)制可能與電針促進(jìn)腦梗死海馬區(qū)e NSCs增殖與分化,抑制e NSCs過(guò)度分化為星形膠質(zhì)細(xì)胞,及早長(zhǎng)時(shí)程促進(jìn)e NSCs分化為神經(jīng)元,促進(jìn)神經(jīng)再生等機(jī)制密切相關(guān)。
[Abstract]:Objective to observe the effect of electroacupuncture on the expression of endogenous neural stem cells (NSCs) in hippocampus of rats after ischemia reperfusion I / R at different time points after middle cerebral artery occlusion (MCAO). To explore the possible mechanism of electroacupuncture (EA) on the distant damage of acute cerebral infarction (ACI). Methods male SD rats of SPF grade were divided into three groups: sham operation group, cerebral infarction group and electroacupuncture group with 30 rats in each group with 30 rats in each group. The sham operation group was treated only with surgical trauma, the cerebral infarction group was treated with MCAO ischemia-reperfusion only, and the electroacupuncture group was treated with "Baihui" and "Dazhui" for 30 minutes, once a day. At the same time, 6 rats were killed at random in each group, the hippocampal tissues of ischemic side were isolated, and the proliferation and differentiation of e NSCs in hippocampus were detected by immunofluorescence method. Results 1) the neurological deficit score of rats was compared with that of sham operation group. Compared with the cerebral infarction group, the nerve function defect score of the electroacupuncture group decreased the expression of P0.05 + BrdU on the 1st day of the 7th day: compared with the sham-operated group, and compared with the sham-operation group, the neurologic deficit score of the electroacupuncture group decreased the expression of P0.05 + BrdU on the 14th day after the first day of cerebral infarction, and compared with the sham operation, there was no significant difference between the two groups. In cerebral infarction group, the number of Brd U positive cells in hippocampal CA1 + CA3 + DG was increased at 714 days after cerebral infarction, and compared with cerebral infarction group, the number of Brd U positive cells in electroacupuncture group was higher than that in cerebral infarction group: compared with sham operation group, the number of Brd U positive cells in electroacupuncture group was higher than that in sham operation group. The number of Nestin positive cells in the dentate gyrus of the cerebral infarction group increased on the 1st day (P 0.05), compared with the cerebral infarction group, the positive expression of Nestin increased in the electroacupuncture group, but on the 7th day there was a significant difference: compared with the sham operation group, the positive expression of Nestin in the electroacupuncture group was higher than that in the sham operation group. Compared with the cerebral infarction group, the expression of DCX positive cells in the electroacupuncture group was higher than that in the sham operation group on day 714, and the expression of the DCX positive cells in the electroacupuncture group was higher than that in the sham operation group, compared with the sham operation group, the expression of DCX positive cells in the electroacupuncture group was higher than that in the sham operation group. The expression of Neu N in dentate gyrus of cerebral infarction group was increased on the 1st day, but on the 14th day, the difference was significant (P 0.05), compared with that in the cerebral infarction group, the expression of Neu N positive cells in electroacupuncture group was higher than that in the cerebral infarction group. However, the expression of GFAP in dentate gyrus of ischemic side in cerebral infarction group was significantly higher than that in sham-operated group on the 1st day after 714 days, compared with that in the cerebral infarction group, and was significantly higher than that in the cerebral infarction group on day 1, 714, and compared with that in the cerebral infarction group, but the expression of GFAP-positive cells in the dentate gyrus of the ischemic side in the cerebral infarction group was significantly higher than that in the sham-operated group. The expression of GFAP positive cells in the electroacupuncture group was not significantly increased, but the difference was significant at the 14th day. Conclusion there is e NSCs proliferation and differentiation in hippocampus after cerebral infarction in rats. Electroacupuncture treatment can promote the recovery of nerve defect function after cerebral infarction. The mechanism may be related to the mechanism of electroacupuncture promoting the proliferation and differentiation of e NSCs in hippocampus of cerebral infarction, inhibiting the overexdifferentiation of e NSCs into astrocytes, promoting the differentiation of e NSCs into neurons early and long term, and promoting nerve regeneration.
【作者單位】： 廣東省佛山市中醫(yī)院神經(jīng)內(nèi)科;南方醫(yī)科大學(xué)南方醫(yī)院神經(jīng)科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(No.81473470,81072947) 廣東省自然科學(xué)基金重點(diǎn)項(xiàng)目(No.8152800007000001,2014A030311033) 佛山市院市合作項(xiàng)目(No.2014HT10004)
【分類號(hào)】：R245;R-332

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