發(fā)布時間：2018-03-19 22:43

  本文選題：流感　切入點：中藥　出處：《中國藥理學(xué)通報》2017年07期 　論文類型：期刊論文

【摘要】：正本課題組前期實驗發(fā)現(xiàn)流感病毒A/FM/1/47(H1N1)引起小鼠死亡或體重百分數(shù)降低,此次實驗采用金蓮花粗提物灌胃的方法,確定金蓮花粗提物的抗病毒作用對小鼠的肺臟的保護作用。1材料與方法1.1病毒株亞洲甲型鼠肺適應(yīng)株A/FM/1/47(H1N1),中國預(yù)防醫(yī)學(xué)科學(xué)院病毒學(xué)研究所提供。血凝滴度1∶512,Reed Muench法測定對小鼠的半數(shù)致死量(LD_(50))10~(-3.68)。1.2主要試劑及儀器金蓮花粗提物(由本課題組其他成員提取獲得),JJ200型電子天平,常熟雙杰測試儀器廠。
[Abstract]:The original preliminary experiments found that influenza virus A/FM/1/47 (H1N1) mice caused by death or weight percentage decreased and the method used in this experiment Jinlianhua crude extract gavage, determine the antiviral effect of Jinlianhua lung extracts on mice and the protective effect of.1 materials and methods of 1.1 influenza virus strains of Asian mouse lung adapted strains (A/FM/1/47 H1N1), China prevention Virology Research Academy of Medical Sciences. The hemagglutination titer of 1: 512, the determination of the lethal dose of half of mouse Reed Muench (LD_ (50)) 10~ (-3.68).1.2 main reagents and instruments Jinlianhua extracts (other members of the group by the extraction, JJ200 electronic) balance, Changshu Shuangjie testing instrument factory.

【作者單位】： 北京中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金資助項目(No 81274044)
【分類號】：R285.5;R-332
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本文編號：1636345