本文選題：卡博替尼　切入點(diǎn)：產(chǎn)單核細(xì)胞李斯特菌　出處：《南方醫(yī)科大學(xué)學(xué)報(bào)》2016年02期 　論文類型：期刊論文

【摘要】：目的探討c-Met受體阻斷劑卡博替尼(Cabozantinib,XL-184)能否抑制產(chǎn)單核細(xì)胞李斯特菌(Listeria monocytogenes,LM)進(jìn)入Caco-2細(xì)胞并降低對(duì)細(xì)胞的損傷。方法通過侵襲實(shí)驗(yàn)研究XL-184在抑制LM進(jìn)入Caco-2細(xì)胞中的作用,并研究劑量及時(shí)間與侵襲率的關(guān)系;Caco-2細(xì)胞種植于Transwell上室,LM感染單層Caco-2細(xì)胞,然后在上室中加入辣根過氧化物酶(HRP),通過測(cè)定跨上皮細(xì)胞電阻(TEER)值及計(jì)數(shù)從上室滲透到下室的LM數(shù)量和HRP濃度,預(yù)測(cè)X-184對(duì)單層細(xì)胞通透性的保護(hù)作用;檢測(cè)乳酸脫氫酶(LDH)釋放水平推測(cè)細(xì)胞膜的滲透性;熒光染色鑒定細(xì)胞活性。結(jié)果相比對(duì)照組,侵襲實(shí)驗(yàn)顯示,XL-184處理組的LM侵襲率明顯降低(P=0.000),且侵襲率隨XL-184濃度和作用時(shí)間的增加而顯著降低,聯(lián)合用藥效果優(yōu)于單獨(dú)用藥(P0.05)。細(xì)胞通透性實(shí)驗(yàn)表明,XL-184可明顯抑制LM誘導(dǎo)的跨上皮細(xì)胞電阻(TEER)的降低(P0.05),并可降低辣根過氧化物酶和細(xì)菌介導(dǎo)的細(xì)胞通透性(P=0.000);細(xì)胞活性實(shí)驗(yàn)表明,XL-184可提高細(xì)胞存活率(P0.01);同時(shí),可顯著降低LM誘導(dǎo)的LDH釋放(P0.05)。結(jié)論 XL-184可能在抑制LM侵襲Caco-2及降低LM誘導(dǎo)的Caco-2損傷中起重要作用。因此,XL-184有望成為潛在的治療和預(yù)防李斯特菌感染的有效藥物。
[Abstract]:Objective to investigate whether c-Met receptor antagonist Cabozantinib (XL-1844) can inhibit the entry of Listeria monocytogenes LMinto Caco-2 cells and reduce the damage to Caco-2 cells. Methods the role of XL-184 in inhibiting LM entry into Caco-2 cells was studied by invasive experiments. The relationship between dose, time and invasion rate was studied. Caco-2 cells were implanted in Transwell LM-infected monolayer Caco-2 cells. Then horseradish peroxidase (HRP) was added to the upper chamber to predict the protective effect of X-184 on the permeability of monolayer cells by measuring the resistance of transepithelial cells and counting the number of LM infiltrating into the lower chamber from the upper chamber and the concentration of HRP. The release level of lactate dehydrogenase (LDH) was measured to speculate the permeability of cell membrane. Fluorescence staining was used to identify the cell activity. The invasion test showed that the invasion rate of LM in XL-184 group was significantly decreased with the increase of XL-184 concentration and action time, and the invasion rate was significantly decreased with the increase of XL-184 concentration and action time. The effect of combined use was better than that of P0.050.The cell permeability test showed that XL-184 could significantly inhibit the decrease of transepithelial cell resistance induced by LM (P0.05N), and could reduce horseradish peroxidase (HRP) and bacteria-mediated cell permeability (P0. 000) and cell viability. The sex experiment showed that XL-184 could improve cell survival rate (P0.01), and at the same time, Conclusion XL-184 may play an important role in inhibiting LM invasion of Caco-2 and reducing LM-induced Caco-2 damage. Therefore, XL-184 may be an effective drug for the treatment and prevention of Listeria sp. Infection.
【作者單位】： 南方醫(yī)科大學(xué)公共衛(wèi)生與熱帶醫(yī)學(xué)學(xué)院//廣東省熱帶病研究重點(diǎn)實(shí)驗(yàn)室微生物學(xué)系;南方醫(yī)科大學(xué)第一臨床醫(yī)學(xué)院;
【基金】：廣州市科技計(jì)劃(2014J4100145) 2015年度國(guó)家級(jí)和省級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201512121026)
【分類號(hào)】：R378

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 龔淑敏;李光明;董莉真;吳志敏;程彬;張斌;朱澤;;慢病毒載體介導(dǎo)的RNAi雙敲除ABCG2和UGT1A1的Caco-2細(xì)胞模型[J];天津醫(yī)科大學(xué)學(xué)報(bào);2013年04期

2 ;[J];;年期

相關(guān)會(huì)議論文 前2條

1 黃曉忠;林正秀;張?zhí)镓S;朱利斌;李仲榮;林錦;;丁酸鈉在Caco-2細(xì)胞腸屏障中的作用及p38MAPK的影響[A];2013年浙江省醫(yī)學(xué)會(huì)小兒外科學(xué)學(xué)術(shù)年會(huì)暨分會(huì)成立30周年慶典論文匯編[C];2013年

2 戴麗娜;侯龍龍;李仲榮;張華;;生理濃度丁酸對(duì)Caco-2細(xì)胞腸屏障作用中JNK通路的效應(yīng)[A];2013年浙江省醫(yī)學(xué)會(huì)小兒外科學(xué)學(xué)術(shù)年會(huì)暨分會(huì)成立30周年慶典論文匯編[C];2013年

，

本文編號(hào)：1615869