本文選題：促紅細(xì)胞生成素　切入點(diǎn)：速發(fā)型哮喘　出處：《毒理學(xué)雜志》2017年05期 　論文類(lèi)型：期刊論文

【摘要】：目的探討促紅細(xì)胞生成素(EPO)對(duì)速發(fā)型哮喘小鼠模型IL-4、IL-5及IgE的變化及肺組織病理變化的相關(guān)機(jī)制影響。方法選擇54只Balb/c小鼠隨機(jī)分為6組,每組9只。哮喘模型組,EPO高、中、低濃度組和地塞米松組,每間隔7 d腹腔注射20μg卵清蛋白(ovalbumin,OVA)+2 mg氫氧化鋁共0.2 ml致敏3次。第22天引喘小鼠,用3%OVA溶液(生理鹽水配制)霧化吸入,吸入30 min/d,持續(xù)7 d。EPO低、中、高濃度組和地塞米松組分別于霧化吸入前30 min腹腔注射低濃度EPO(500 IU/kg)、中濃度EPO(1 000 IU/kg)、高濃度EPO(2 000 IU/kg)和地塞米松(1 mg/kg)。正常對(duì)照組腹腔注射和霧化吸入的時(shí)間和方法相同只是使用生理鹽水替代藥品。通過(guò)酶聯(lián)免疫吸附實(shí)驗(yàn)法(ELISA)檢測(cè)血清中IL-5、IL-4和IgE水平;小鼠肺組織行切片,蘇木素-伊紅(HE)染色,觀(guān)察其病理變化。結(jié)果EPO高、中、低濃度組,哮喘模型組和地塞米松組IL-4、IL-5及IgE濃度均高于正常對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。EPO高、中濃度組和地塞米松組IL-4、IL-5及IgE濃度均低于哮喘模型組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。正常對(duì)照組中小鼠支氣管的上皮無(wú)損傷,炎癥反應(yīng)表現(xiàn)不明顯,且肺組織形態(tài)無(wú)異常;而哮喘模型組中小鼠存在很多的炎性細(xì)胞,其中大部分存在于支氣管及其周?chē)?最終致使管腔狹窄;EPO低濃度組結(jié)果顯示存在的炎性細(xì)胞及管腔狹窄程度較哮喘組減輕,隨EPO濃度的增加,病理?yè)p傷程度減輕;EPO高、中和低濃度組與地塞米松組比較,管腔及平滑肌厚度相似,但地塞米松組炎性細(xì)胞浸潤(rùn)程度明顯減輕。結(jié)論 EPO可抑制哮喘小鼠IL-4、IL-5、IgE的分泌及減輕肺組織病理改變,提示EPO對(duì)哮喘小鼠模型的炎癥因子的產(chǎn)生具有調(diào)節(jié)作用。
[Abstract]:Objective to investigate the effects of erythropoietin (EPO) on the changes of IL-4 IL-5 and IgE and the pathological changes of lung tissue in mouse model of acute asthma. Methods 54 Balb/c mice were randomly divided into 6 groups with 9 in each group. In the low concentration group and dexamethasone group, 20 渭 g ovalvalbumin OVA (20 渭 g ovalbumin OVA) 2 mg aluminum hydroxide was injected intraperitoneally every 7 days for 3 times. The high concentration group and dexamethasone group were injected intraperitoneally with low concentration of EPO(500 / kg, moderate concentration of EPO(1 / kg, high concentration of EPO(2 / kg / kg) and dexamethasone 1 mg / kg respectively at 30 min before atomization inhalation. The time and method of intraperitoneal injection and inhalation of dexamethasone were the same in normal control group. The serum levels of IL-5 IL-4 and IgE were detected by Elisa. Results the levels of IL-4 IL-5 and IgE in the high, middle and low EPO groups, asthma model group and dexamethasone group were higher than those in the normal control group, and the difference was statistically significant. The concentrations of IL-4 IL-5 and IgE in the middle concentration group and dexamethasone group were lower than those in the asthmatic model group (P 0.05). However, there were many inflammatory cells in the asthmatic model group, most of which were found in the bronchi and its surrounding blood vessels, which resulted in the lower concentration of EPO in the lumen stenosis and the degree of the inflammatory cells and the stenosis of the lumen were less than those in the asthmatic group. With the increase of EPO concentration, the degree of pathological injury was reduced. The thickness of lumen and smooth muscle was similar in medium and low concentration groups compared with dexamethasone group. Conclusion EPO can inhibit the secretion of IL-4 IL-5 IgE and alleviate the pathological changes of lung tissue in asthmatic mice, suggesting that EPO can regulate the production of inflammatory factors in asthmatic mice.
【作者單位】： 延邊大學(xué)附屬醫(yī)院;
【分類(lèi)號(hào)】：R-332;R562.25

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