本文關(guān)鍵詞： 多房棘球蚴 膽汁酸協(xié)同轉(zhuǎn)運(yùn)蛋白 基因 克隆 生物信息學(xué) 分析　出處：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年08期 　論文類型：期刊論文

【摘要】：目的 膽汁酸鈉協(xié)同轉(zhuǎn)運(yùn)蛋白在棘球絳蟲發(fā)育過(guò)程中起著重要作用。文中研究克隆多房棘球蚴(Em)的膽汁酸鈉協(xié)同轉(zhuǎn)運(yùn)蛋白5(Em SBACT5)基因,并對(duì)其編碼蛋白進(jìn)行生物信息學(xué)分析。方法 采用RT-PCR技術(shù)擴(kuò)增Em SBACT5基因并測(cè)序,采用生物信息學(xué)軟件對(duì)編碼蛋白的理化性質(zhì)、親/疏水性、跨膜域、翻譯后修飾位點(diǎn)、結(jié)構(gòu)域、二級(jí)結(jié)構(gòu)、三級(jí)結(jié)構(gòu)、亞細(xì)胞定位和生物學(xué)功能進(jìn)行預(yù)測(cè)分析。結(jié)果 擴(kuò)增出654 bp的完整開放閱讀框,其編碼217個(gè)氨基酸,與公布的細(xì)粒棘球蚴(Eg)的Eg SBACT5基因核苷酸和氨基酸同源性分別達(dá)98%和96%。蛋白分析結(jié)果顯示,Em SBACT5蛋白分子式為C1141H1797N273O284S11,相對(duì)分子量為24 240,p I為8.99。含有9個(gè)翻譯后修飾位點(diǎn)和4處典型的結(jié)構(gòu)域。α-螺旋、β-折疊、β-轉(zhuǎn)角和無(wú)規(guī)則卷曲所占的比例分別為29.95%、31.80%、7.83%和30.41%。該蛋白為疏水性跨膜蛋白,主要定位于細(xì)胞質(zhì)膜中,可能在物質(zhì)轉(zhuǎn)運(yùn)和信號(hào)傳遞過(guò)程中發(fā)揮作用。結(jié)論 成功克隆出Em SBACT5基因并獲得其編碼蛋白的的信息學(xué)特征,為包蟲病防治提供基礎(chǔ)信息。
[Abstract]:Objective Sodium bile acid co-transporter plays an important role in the development of Echinococcus multilocularis. In this paper, the gene of sodium bile acid co-transporter 5Em SBACT5 was cloned from Echinococcus multilocularis (Echinococcus multilocularis). Methods the Em SBACT5 gene was amplified by RT-PCR and sequenced. The physicochemical properties, hydrophilicity, transmembrane domain, post-translational modification site and domain of the encoded protein were analyzed by bioinformatics software. The secondary structure, tertiary structure, subcellular localization and biological function were predicted and analyzed. Results A complete open reading frame of 654 BP was amplified, encoding 217 amino acids. The nucleotide and amino acid homology of Eg SBACT5 gene of Echinococcus granulosus Egg was 98% and 96.The protein analysis showed that the molecular formula of Em SBACT5 protein was C1141H17N273O284S11 and the relative molecular weight was 24.240pI = 8.99.The protein analysis showed that the Em SBACT5 protein contained nine posttranslational modification sites. The proportion of 偽 -helix, 尾 -fold, 尾 -corner and irregular curl were 29.95, 31.807.83% and 30.41% respectively. The protein was hydrophobic transmembrane protein. Conclusion the Em SBACT5 gene was cloned successfully and the informatics characteristics of its encoded protein were obtained, which provided the basic information for the prevention and control of hydatid disease.
【作者單位】： 青海大學(xué)生態(tài)環(huán)境工程學(xué)院;青海大學(xué)附屬醫(yī)院教學(xué)管理部;青海大學(xué)附屬醫(yī)院肝膽胰外科;青海大學(xué)附屬醫(yī)院燒傷科;青海大學(xué)醫(yī)學(xué)院生化教研室;
【分類號(hào)】：R383.3

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