本文關(guān)鍵詞： 五羥黃酮 肺纖維化 博萊霉素　出處：《廣東醫(yī)學(xué)》2017年15期 　論文類型：期刊論文

【摘要】：目的探討五羥黃酮對(duì)博萊霉素(BLM)誘導(dǎo)大鼠肺纖維化模型的預(yù)防及治療作用。方法將雄性C57BL/6小鼠隨機(jī)分為假手術(shù)組、BLM模型組(80 mg/kg)、五羥黃酮小劑量組(40 mg/kg)、五羥黃酮大劑量組(80mg/kg)、地塞米松(DXM)組,造模后第10天,靜脈給藥20 d,于造模后第30天處死動(dòng)物、取材。通過(guò)病理學(xué)檢查及肺組織羥脯氨酸含量確定肺纖維損傷及程度;測(cè)定肺泡支氣管灌洗液(BALF)蛋白含量反映炎性細(xì)胞因子的釋放;測(cè)定致炎性細(xì)胞因子腫瘤壞死因子-α(TNF-α)在BALF的水平及在肺組織的表達(dá),以及促纖維化細(xì)胞因子血小板源性生長(zhǎng)因子(PDGF-BB)和轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)在肺組織的表達(dá),以探討五羥黃酮防治肺纖維化的可能機(jī)制。結(jié)果與BLM模型組相比,五羥黃酮40及80 mg/kg均明顯緩解實(shí)驗(yàn)第7及30天的炎癥細(xì)胞浸潤(rùn)、膠原纖維形成等病理學(xué)改變,顯著降低纖維化面積,并降低實(shí)驗(yàn)第30天羥脯氨酸含量。五羥黃酮大劑量組還可降低實(shí)驗(yàn)第7及30天BLM引起的BALF中蛋白含量的升高。對(duì)于致炎性細(xì)胞因子TNF-α,在實(shí)驗(yàn)第7及30天,五羥黃酮小、大劑量組BALF中其含量及肺組織中其表達(dá)水平均較BLM模型組低;對(duì)于促纖維化細(xì)胞因子TGF-β1和PDGF-BB,與BLM模型組相比,五羥黃酮40及80 mg/kg顯著降低實(shí)驗(yàn)第30天肺組織中兩者的表達(dá)。結(jié)論五羥黃酮對(duì)BLM誘導(dǎo)的肺纖維化模型具有預(yù)防及治療作用,可能與其下調(diào)肺組織TNF-α、TGF-β1和PDGF-BB的表達(dá)有關(guān)。
[Abstract]:Objective to investigate the preventive and therapeutic effects of pentaflavone on bleomycin induced pulmonary fibrosis in rats. Methods male C57BL / 6 mice were randomly divided into sham-operation group (n = 80 mg / kg), low dose group (n = 40 mg / kg) and pentaflavone group (n = 40 mg / kg). The dose group was 80 mg / kg, and the dexamethasone DXM group, On the 10th day, 20 days after intravenous administration, the animals were killed on the 30th day, and the lung fiber injury and degree were determined by pathological examination and the content of hydroxyproline in lung tissue. The content of BALF- protein in bronchoalveolar lavage fluid (BALF) was determined to reflect the release of inflammatory cytokines, the expression of TNF- 偽 in BALF and the expression of TNF- 偽 in lung tissue were measured. And the expression of PDGF-BBand TGF- 尾 _ 1 in lung tissue to explore the possible mechanism of pentaflavone in the prevention and treatment of pulmonary fibrosis. Results compared with BLM model group, the expression of PDGF-BBB and TGF- 尾 _ 1 in lung tissue was studied. At 40 and 80 mg/kg, pentaflavone significantly alleviated the inflammatory cell infiltration, collagen fiber formation and other pathological changes on the 7th and 30th days of the experiment, and significantly reduced the area of fibrosis. It also decreased the content of hydroxyproline on the 30th day of the experiment. The increase of protein content in BALF induced by BLM on the 7th and 30th day was also decreased in the high-dose group. For the inflammatory cytokine TNF- 偽, on the 7th and 30th days, the pentaflavone was small. The content of BALF and the expression of TGF- 尾 1 and PDGF-BBB in BALF and lung tissue in high dose group were lower than those in BLM model group, and compared with BLM model group, the levels of TGF- 尾 1 and PDGF-BBs in high dose group were lower than those in BLM model group. Conclusion Pentaflavone can prevent and treat pulmonary fibrosis induced by BLM, which may be related to its down-regulation of the expression of TNF- 偽 -TGF- 尾 1 and PDGF-BB in lung tissue.
【作者單位】： 黃河科技學(xué)院醫(yī)學(xué)院;鄭州大學(xué)第一附屬醫(yī)院肝膽外科;
【基金】：河南省醫(yī)學(xué)教育研究課題(編號(hào):wjlx2015197)
【分類號(hào)】：R285.5;R-332
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本文編號(hào)：1548459