本文關(guān)鍵詞： 犬 疾病模型 卵母細(xì)胞 體外成熟　出處：《中國實(shí)驗(yàn)動物學(xué)報(bào)》2017年01期 　論文類型：期刊論文

【摘要】：國內(nèi)實(shí)驗(yàn)Beagle犬種質(zhì)資源保存利用及制備基因修飾人類疾病動物模型,要求有充足的Beagle犬胚胎。目前誘導(dǎo)排卵技術(shù)在犬上效果不明顯,體內(nèi)獲取犬成熟卵母細(xì)胞有困難。同時(shí),盡管科研人員對犬卵母細(xì)胞體外成熟培養(yǎng)進(jìn)行了多方面的嘗試,但尚未獲得突破,成熟率低,嚴(yán)重制約了其在種質(zhì)資源保存、基因修飾模型制備及生物醫(yī)學(xué)研究中的應(yīng)用。本文梳理了不同犬齡及生殖周期、卵母細(xì)胞形態(tài)與體積及脂滴在Beagle犬卵母細(xì)胞體外成熟中的影響,以為其體外成熟尋找新的突破口。
[Abstract]:Preservation and utilization of experimental Beagle dog germplasm resources and preparation of genetically modified animal models of human disease require sufficient Beagle dog embryos. At present, the technique of ovulation induction is not effective in dogs, so it is difficult to obtain mature canine oocytes in vivo. Although researchers have tried in many ways to mature canine oocytes in vitro, they have not made a breakthrough and have a low rate of maturation, which seriously restricts the preservation of canine oocytes in germplasm resources. The effects of age, reproductive cycle, oocyte morphology and volume and lipid droplets on the maturation of Beagle canine oocytes in vitro were studied. For its in vitro maturation to find a new breakthrough.
【作者單位】： 廣州醫(yī)藥研究總院有限公司實(shí)驗(yàn)動物研究開發(fā)中心國家犬類實(shí)驗(yàn)動物種子中心;
【基金】：廣州市“珠江科技新星”項(xiàng)目(201610010144) 廣州市科技基礎(chǔ)條件平臺建設(shè)項(xiàng)目(201605040005)
【分類號】：R-332

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