本文關(guān)鍵詞： IP3 膳食鈣 胞內(nèi)鈣 脂肪沉積　出處：《西北農(nóng)林科技大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：鈣在動物脂肪代謝中發(fā)揮著重要作用,膳食鈣被證實有抗肥胖的作用,在對其作用機制的研究中,人們認為外源鈣以膳食形式攝入體內(nèi),會改變體內(nèi)細胞的鈣濃度,使鈣信號發(fā)生變化,從而引發(fā)一系列生物學(xué)效應(yīng)。通過IP3通路鈣庫可以使胞內(nèi)鈣離子濃度升高,引起鈣信號改變,但是目前對胞內(nèi)鈣濃度變化的研究主要集中在肌肉收縮和心血管疾病上,有關(guān)脂肪代謝的研究幾乎沒有。 本研究以小鼠為實驗動物,分別在肥胖和正常狀態(tài)下用藥物處理,小鼠隨機分為對照組、去甲腎上腺素(NE)組、肝素組、鈣組、鈣+NE組和鈣+肝素組。分析指標包括:體重、采食量、組織重、血脂水平、組織鈣含量和組織中脂代謝相關(guān)基因mRNA和蛋白表達。旨在觀察鈣庫和膳食鈣引發(fā)的胞內(nèi)鈣濃度變化對脂肪代謝的影響。結(jié)果如下: 1.試驗發(fā)現(xiàn),肝素和膳食鈣處理肥胖小鼠有顯著的減肥效果(P0.01),減少體脂含量(P0.01),血清中TG和TC含量顯著降低(P0.01),HDL-C水平卻升高(P0.01),并且PPARγ、FAS和IP3R1的mRNA水平均降低(P0.01),HSLmRNA水平顯著升高(P0.01),C/EBPα的mRNA水平無明顯變化;去甲腎上腺素處理肥胖小鼠,效果與肝素相反,明顯削弱減肥作用(P0.01);相比對照組,肝素組、鈣組、鈣+NE組和鈣+肝素組的PPARγ、C/EBPα和FAS的蛋白表達量有所減少,ATGL的表達量有所增加;NE組的PPARγ、C/EBPα和FAS的蛋白表達量增大,而在ATGL上的蛋白表達量下降。膳食鈣有顯著減肥效果(P0.01),但鈣+NE組的減肥效果弱于鈣組(P0.01),鈣+肝素組的減肥效果強于膳食鈣的處理效果(P0.01)。說明IP3通路激活可引起胞內(nèi)鈣離子升高,脂肪蓄積增加;反之,脂肪蓄積減少。通過IP3通路,膳食鈣可抑制胞內(nèi)鈣離子增加。 2.結(jié)果表明,NE、肝素和膳食鈣處理正常小鼠,都表現(xiàn)出了對小鼠的體質(zhì)量的抑制作用(P0.01),減少了附睪脂肪和腎周脂肪的重量(P0.01),增加了棕色脂肪組織重量(P0.01),血清中TG和TC含量顯著降低(P0.01),HDL-C水平卻升高(P0.01);NE可以增加IP3R1mRNA的表達量(P0.01),肝素和鈣抑制了IP3R1mRNA的表達(P0.01)。NE、肝素和膳食鈣的FAS、PPARγ和C/EBPα的mRNA均表現(xiàn)極顯著下降(P0.01),HSL的mRNA表達顯著上升(P0.01);NE組、肝素組、鈣組、鈣+NE組和鈣+肝素組的PPARγ、C/EBPα和FAS的蛋白含量降低;NE組、肝素組、鈣組、鈣+NE組和鈣+肝素組的ATGL蛋白含量升高。說明膳食鈣通過IP3通路可抑制胞內(nèi)鈣離子增加,對動物體的脂肪代謝有抑制作用;動物體棕色脂肪組織可以極大的限制肥胖的發(fā)生。
[Abstract]:Calcium plays an important role in animal fat metabolism. Dietary calcium has been proved to have anti-obesity effect. In the study of its mechanism, it is believed that exogenous calcium intake in vivo will change the calcium concentration of cells in vivo. Through the IP3 pathway, calcium can increase the intracellular calcium concentration and cause the change of calcium signal. However, the current studies on intracellular calcium concentration mainly focus on muscle contraction and cardiovascular disease, and there are few studies on fat metabolism. In this study, mice were treated with drugs in obese and normal conditions. The mice were randomly divided into three groups: control group, NE group, heparin group, calcium group, calcium NE group and calcitonin group. The effects of calcium intake, tissue weight, blood lipid level, tissue calcium content and lipid metabolism-related genes mRNA and protein expression on lipid metabolism were observed in order to observe the effects of calcium pool and calcium induced changes in intracellular calcium concentration on lipid metabolism. 1. The experiment found that. Heparin and dietary calcium treatment could significantly reduce the weight loss of obese mice (P 0.01), decrease body fat content (P 0.01), decrease serum TG and TC (P 0.01), but increase HDL-C (P 0.01). However, the mRNA levels of PPAR 緯 FAS and IP3R1 decreased significantly (P 0.01) and HSL mRNA level increased significantly (P 0.01%), while the mRNA level of P0.01C / EBP 偽 did not change significantly. Compared with control group, heparin group, calcium group, heparin group, noradrenaline treated obese mice, the effect was contrary to heparin, significantly weakened the weight loss effect of P0.01, compared with control group, heparin group, calcium group, The protein expression of PPAR 緯 -C / EBP 偽 and FAS in calcium NE group and calcium heparin group was decreased. The expression of PPAR 緯 -C% EBP 偽 and FAS in NE group was increased, and the protein expression of PPAR 緯 -C% EBP 偽 and FAS increased in NE group. However, the protein expression on ATGL decreased. Dietary calcium had a significant effect on weight loss, but the effect of calcium NE group was weaker than that of calcium group P0.01, and that of calcium heparin group was stronger than that of dietary calcium treatment. The results showed that activation of IP3 pathway could cause the activation of IP3 pathway. Intracellular calcium increased, Fat accumulation increased, whereas fat accumulation decreased. Dietary calcium inhibited the increase of intracellular calcium ion through IP3 pathway. 2. The results showed that normal mice were treated with heparin and dietary calcium. All of them showed inhibitory effect on the body weight of mice, decreased the weight of epididymal fat and perirenal fat, increased the weight of brown adipose tissue and increased the weight of brown adipose tissue. The levels of TG and TC in serum decreased significantly the level of HDL-C of P0.01C, but increased the level of P0.01C, which increased IP3R1mRNA. The expression of IP3R1mRNA was inhibited by heparin and calcium. The mRNA of FASPPAR 緯 and C / EBP 偽 in heparin and dietary calcium decreased significantly. The protein contents of PPAR 緯 C / EBP 偽 and FAS in heparin group, calcium group, calcium NE group and calcium heparin group decreased in NE group, heparin group and calcium group, respectively. The content of ATGL protein in calcium NE group and calcium heparin group was increased, which indicated that dietary calcium could inhibit the increase of intracellular calcium ion through IP3 pathway and inhibit fat metabolism in animals, while brown adipose tissue in animal body could greatly limit the occurrence of obesity.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

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