本文關鍵詞： 肺癌 皮下移植瘤 動物模型　出處：《中國實驗動物學報》2017年04期 　論文類型：期刊論文

【摘要】：目的探究不同部位肺癌皮下移植瘤存在的差異,為肺癌研究者提供提供基礎依據(jù)。方法將穩(wěn)定表達蟲螢光素酶的小鼠Lewis肺腺癌細胞(ll2-luc-m38)分別注射于C57 BL/6小鼠的右腋皮下、右腹股溝皮下、腳墊皮下,定期利用小動物活體成像系統(tǒng)觀察小鼠皮下移植瘤成瘤情況及轉移情況,觀察不同部位荷瘤小鼠的生存時間和死亡率,腫瘤組織取材,采用石蠟包埋、切片、HE染色做出病理學診斷。并將小鼠處死后立即進行肺部組織固定、觀察轉移灶。對皮下移植瘤行切除術觀察小鼠術后生存情況和轉移情況。結果腋下組和腹股溝組成瘤時間較早,成瘤率為100%,腳墊組成瘤時間較晚,成瘤率為33%;腹股溝組和腋下組瘤體積增長迅速,其中腹股溝組瘤體積增長最快;接種后第21天腋下組70%的小鼠出現(xiàn)了肺轉移,轉移灶數(shù)目較多;腹股溝組50%小鼠出現(xiàn)肺轉移,且轉移灶數(shù)目較少;腳墊組小鼠未觀察到肺轉移灶。腳墊組小鼠死亡率最高。腹股溝組和腋下組小鼠可行皮下移植瘤切除術,術后存活率為100%。結論小鼠lewis肺癌皮下移植瘤模型中,腹股溝組和腋下組成瘤率高,可耐受移植瘤切除術,手術死亡風險低,便于監(jiān)測,操作簡單且可重復性高,其中腋下組轉移性更好。
[Abstract]:To explore the differences exist in different parts of the lung cancer transplanted subcutaneously to provide the basis for the study of lung cancer. Methods the stable expression of luciferase in mice Lewis lung adenocarcinoma cells (ll2-luc-m38) were injected into C57 BL/6 mice subcutaneously in the right axilla and right inguinal subcutaneous, subcutaneous pad, regular mice transplanted subcutaneously into the condition of tumor and metastasis with small animal imaging system, observe the survival time and mortality in different parts of the tumor bearing mice, tumor tissue samples, using paraffin section, HE staining, and make pathological diagnosis. The mice were sacrificed immediately after lung tissue fixation, metastasis and metastasis. To observe the survival of skin tumor resection were observed after operation. Results the armpit and groin group consisting of tumor earlier, tumor formation rate was 100%, the Ottomans oncogenous late time, tumor was 33%; inguinal Group and lat group tumor volume increased rapidly, which is the fastest growing group of groin tumor volume; twenty-first days after inoculation of axillary group 70% mice developed lung metastasis, metastasis number; group 50% mice inguinal metastasis occurred, and the number of metastases is less; the Ottomans were not observed in mice lung metastasis. The highest mat group the mortality of mice. Mice were viable group and inguinal axillary subcutaneous transplantation tumor resection, postoperative survival rate was 100%. conclusion the mouse Lewis lung cancer xenograft model, inguinal and axillary group tumor formation rate is high, can withstand tumor excision surgery, the risk of death is low, easy monitoring, simple operation and high repeatability among them, axillary metastatic group better.

【作者單位】： 北京中醫(yī)藥大學;首都醫(yī)科大學附屬北京中醫(yī)醫(yī)院;
【基金】：國家自然科學基金項目(編號:81473643) 首都醫(yī)科大學基礎-臨床科研合作基金項目(編號:15JL79) 北京市醫(yī)院管理局青苗計劃項目(編號:QML20150903)
【分類號】：R-332;R734.2

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本文編號：1530271