本文關(guān)鍵詞： 慢性非細(xì)菌性前列腺炎 動物模型 角叉菜膠　出處：《中國實驗動物學(xué)報》2017年05期 　論文類型：期刊論文

【摘要】：目的建立化學(xué)性慢性非細(xì)菌性前列腺炎(chronic non-bacterial prostatitis,CNP)動物模型,為CNP的發(fā)病機制及藥物研究提供可靠的模型動物及評價方法。方法將SD雄性大鼠隨機分為對照組和模型A、B、C組,模型A、B、C組分別用1%的無菌角叉菜膠注射至前列腺左右腹側(cè)葉中各20、50、100μL;對照組每只注入50μL滅菌的生理鹽水溶液。7 d后處理,分別從解剖學(xué)角度、前列腺指數(shù)、白細(xì)胞檢測、病理切片以及腫瘤壞死因子-α(TNF-α)、核因子-κB(NF-κB)、IκB激酶(IKKα)、磷酸化IKB-α(p-IKB-α)和環(huán)氧合酶-2(COX-2)蛋白表達(dá)的影響5個方面進(jìn)行分析。結(jié)果與假手術(shù)組比較,模型A、B、C組的前列腺組織柔軟程度下降,彈性減弱,與周圍組織發(fā)生粘連;前列腺指數(shù)、白細(xì)胞總數(shù)均顯著增加(P0.01),A、B、C組前列腺指數(shù)的增加率分別為21.1%、61.7%和72.7%;白細(xì)胞總數(shù)增加率分別為75.0%、103.6%和114.8%;病理結(jié)果顯示A組前列腺間質(zhì)腫脹較少,B、C組組間質(zhì)明顯腫脹,C組前列腺萎縮明顯,部分腺體變性壞死;A、B、C組前列腺組織內(nèi)NF-κB、IKKα、p-IKB-α、TNF-α和COX-2表達(dá)水平出現(xiàn)不同程度的增加,NF-κB炎癥通道被激活。結(jié)論大鼠前列腺左右腹側(cè)葉注射1%的角叉菜膠各20、50、100μL,均能夠誘導(dǎo)不同程度前列腺炎癥反應(yīng),但20μL劑量太小,炎癥反應(yīng)較弱,操作誤差大;100μL炎癥反應(yīng)過強,有一定動物死亡;50μL劑量所造成的前列腺炎動物模型最為成功,并可明顯激活NF-κB炎癥信號通路。
[Abstract]:Objective to establish an animal model of chronic non-bacterial prostatitis in order to provide reliable animal model and evaluation method for the pathogenesis and drug study of CNP. Methods Sprague-Dawley male rats were randomly divided into two groups: control group and model group. The model group A was injected with 1%% aseptic carrageenin into the ventral lobe of the prostate, and the control group was treated with 50 渭 L sterilized saline solution for 7 days, and the prostate index and white blood cell were measured from the anatomic point of view. Pathological sections and the expression of TNF- 偽, NF- 魏 B, phosphorylated IKB- 偽, phosphorylated IKB- 偽 and cyclooxygenase-2 were analyzed. Results compared with sham-operated group, the tissue softness of prostate was decreased in the model group. Reduced elasticity, adhesion to surrounding tissue; prostate index, The increase rate of prostatic index in group C was 21.1% and 72.7%, respectively, and the increase rate of total leukocyte count was 75.0% and 114.8%, respectively. The pathological results showed that the interstitial tumefaction in group A was less than that in group B C, and the prostatic atrophy in group C was obvious. The expression levels of NF- 魏 BK 偽 -IKK 偽 -IKB- 偽 -IKB- 偽 and TNF- 偽 and COX-2 were increased to varying degrees in the prostatic tissues of partial adenodegenerative necrotic group. Conclusion the inflammatory channels of NF- 魏 B are activated by injecting 1% carrageenin into the left and right ventral lobes of the prostate in rats, all of which can be induced to be different. Degree of prostatic inflammation, However, the dose of 20 渭 L was too small, the inflammatory response was weak, and the inflammatory response of 100 渭 L was too strong. The animal model of prostatitis caused by a certain dose of 50 渭 L of animal death was the most successful, and the NF- 魏 B inflammatory signal pathway could be activated obviously.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院藥用植物研究所;廣西中醫(yī)藥大學(xué)藥學(xué)院;哈爾濱商業(yè)大學(xué);廣西藥用植物園;
【分類號】：R-332;R697.33

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