本文關(guān)鍵詞： ABCE 肺腺癌 動物模型 免疫組化檢測　出處：《現(xiàn)代腫瘤醫(yī)學(xué)》2016年06期 　論文類型：期刊論文

【摘要】：目的:建立ABCE1-GFP(+)肺腺癌裸鼠動物模型,并進(jìn)行免疫組化檢測,為進(jìn)一步探索ABCE1作為診斷治療非小細(xì)胞肺癌靶點提供有用的工具。方法:應(yīng)用前期實驗中構(gòu)建的穩(wěn)定轉(zhuǎn)染重組質(zhì)粒p EGFPC1-ABCE1及空載體p EGFP-C1的LTEP-a-2肺腺癌細(xì)胞株,接種于裸鼠背部側(cè)翼至腋窩皮下,建立ABCE1-GFP肺腺癌動物模型。進(jìn)行成瘤情況觀察,并予以倒置熒光顯微鏡系統(tǒng)呈像。取材后進(jìn)行免疫組織化學(xué)檢測。結(jié)果:穩(wěn)轉(zhuǎn)細(xì)胞系皮下接種裸鼠后,全部成瘤,過表達(dá)組腫瘤體積大于空載體及對照組(P0.05)。過表達(dá)組腫瘤在熒光顯微鏡系統(tǒng)下可穩(wěn)定表達(dá)綠色熒光,成功構(gòu)建了ABCE1-GFP(+)肺腺癌皮下成瘤的裸鼠動物模型。免疫組織化學(xué)檢測亦驗證這一模型的確立。結(jié)論:穩(wěn)定轉(zhuǎn)染LTEP-a-2肺腺癌細(xì)胞可以用來成功建立皮下ABCE1-GFP(+)肺腺癌成瘤模型,是較為理想研究ABCE1在非小細(xì)胞肺癌中的基因功能的動物模型。
[Abstract]:Objective: to establish the nude mice model of ABCE1-GFP() lung adenocarcinoma and to detect it by immunohistochemistry. To further explore ABCE1 as a target for the diagnosis and treatment of non-small cell lung cancer (NSCLC) methods: the recombinant plasmid p#en1# and the empty vector p#en2# of LTEP-a-2 lung adenocarcinoma cell line, which were constructed in previous experiments, were used for the diagnosis and treatment of NSCLC. The animal model of ABCE1-GFP lung adenocarcinoma was established by inoculating the dorsal flanking to axillary subcutaneous of nude mice. The fluorescence microscope system was inverted and the samples were taken for immunohistochemical examination. Results: after subcutaneous inoculation of the stable transformed cell line, all of them were tumorigenic. The tumor volume of the overexpression group was larger than that of the empty vector and the control group (P 0.05). The tumor in the overexpression group could express green fluorescence stably under the fluorescence microscope system. A nude mouse model of subcutaneous tumorigenesis of lung adenocarcinoma ABCE1-GFP() was successfully constructed, and the establishment of this model was also verified by immunohistochemistry. Conclusion: stable transfection of LTEP-a-2 can be used to successfully establish subcutaneous ABCE1-GFP (lung adenocarcinoma) tumorigenic model. It is an ideal animal model to study the gene function of ABCE1 in non-small cell lung cancer.
【作者單位】： 中國醫(yī)科大學(xué)附屬第四醫(yī)院胸外科;
【基金】：國家自然科學(xué)基金資助項目(編號:30973502) 中國醫(yī)科大學(xué)附屬第四醫(yī)院青年創(chuàng)新發(fā)展計劃基金資助項目(編號:院發(fā)[2013]57號)
【分類號】：R734.2;R-332

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