本文關鍵詞： 細胞低氧 骨形態(tài)發(fā)生蛋白質類 間質干細胞 骨髓 細胞分化 兔　出處：《脊柱外科雜志》2016年02期 　論文類型：期刊論文

【摘要】：目的研究缺氧狀態(tài)對骨形態(tài)發(fā)生蛋白-2(BMP-2)誘導分化體外復合富血小板血漿(PRP)凝膠的骨髓間充質干細胞(BMSCs)的影響。方法取第三代培養(yǎng)的BMSCs細胞與PRP凝膠相混合,通過構建細胞缺氧模型,根據(jù)培養(yǎng)條件的不同確定實驗分組:常氧BMSCs+PRP組(A組)、常氧BMP-2+BMSCs+PRP組(B組)、低氧BMSCs+PRP組(C組)、低氧BMP-2+BMSCs+PRP組(D組)。反轉錄-聚合酶鏈反應法(RT-PCR)及Western-blotting檢測各組成軟骨及成骨基因的表達。結果 RT-PCR檢測表明C組和D組的Ⅱ型膠原、蛋白聚糖和低氧誘導因子-1α(HIF-1α)表達均較A組和B組明顯升高;Ⅰ型膠原和堿性磷酸酶(ALP)在B組的表達最高;A組與B組runt相關轉錄因子2(Runx2)的表達較C組和D組明顯升高。Western-blotting檢測結果表明B組和D組的Ⅱ型膠原表達較A組和C組明顯升高;C組與D組的HIF-1α表達較A組與B組顯著升高;D組的SOX-9表達較其余3組顯著升高。所有結果差異均有統(tǒng)計學意義(P0.05)。結論低氧條件能顯著促進BMP-2對BMSCs的成軟骨誘導分化,同時抑制BMSCs的成骨分化。HIF-1α可能是這一過程的重要調節(jié)因子,其可能通過調節(jié)SOX-9的表達來發(fā)揮促BMSCs成軟骨細胞分化的作用。
[Abstract]:Objective to study the effect of hypoxia on bone morphogenetic protein-2 BMP-2 (BMP-2) -induced differentiation of bone marrow mesenchymal stem cells (BMSCs) combined with platelet-rich plasma (PRP) gel in vitro. Methods BMSCs cells cultured in the third generation were mixed with PRP gel. According to the different culture conditions, the cells were divided into two groups: normoxic BMSCs PRP group (group A), normoxic BMP-2 BMSCs group (group B) and normoxic BMP-2 BMSCs group (group B). Hypoxia BMSCs PRP group (group C). Reverse transcription-polymerase chain reaction (RT-PCR) in hypoxic BMP-2 BMSCs PRP group. Results the expression of osteogenic genes in cartilage and osteogenesis was detected by Western-blotting. Results the type 鈪，

本文編號：1469371