本文關(guān)鍵詞： 華支睪吸蟲 布氏姜片吸蟲 單染法 復(fù)染法　出處：《中國(guó)寄生蟲學(xué)與寄生蟲病雜志》2016年04期 　論文類型：期刊論文

【摘要】：采用醋酸卡紅溶液和孔雀綠水溶液,對(duì)固定后的華支睪吸蟲和布氏姜片蟲標(biāo)本進(jìn)行復(fù)合染色。醋酸卡紅對(duì)兩種吸蟲標(biāo)本進(jìn)行單染。復(fù)染法使得標(biāo)本蟲體呈三色,消化系統(tǒng)、排泄系統(tǒng)和周圍肌肉組織均為淺紅色,生殖器官中的子宮呈現(xiàn)鮮綠色,蟲體兩側(cè)卵黃腺為黃褐色,蟲體結(jié)構(gòu)清晰、著色適度、立體感強(qiáng)。醋酸卡紅染液?jiǎn)稳緦?duì)吸蟲睪丸組織的顯現(xiàn)力更強(qiáng)。在吸蟲的教學(xué)和科研工作中,結(jié)合使用單染法和復(fù)染法,觀察效果將會(huì)更好。
[Abstract]:The specimens of Clonorchis sinensis and turmeria brinsoni were stained with carmine acetate solution and malachite green solution. The specimens of two species of paragonimiasis were singly stained by carmine acetate, and the specimens were trichromatic by the method of restaining. The digestive system, excretory system and surrounding muscle tissue were all light red, the uterus in reproductive organs was bright green, the yolk glands on both sides of insect body were yellowish brown, the structure of insect body was clear and the staining was moderate. In teaching and scientific research of trematodes, the observation effect will be better if the method of single staining and double staining is combined with the method of single staining and double staining.
【作者單位】： 皖南醫(yī)學(xué)院醫(yī)學(xué)影像與檢驗(yàn)學(xué)院;皖南醫(yī)學(xué)院醫(yī)學(xué)寄生蟲學(xué)教研室;
【基金】：皖南醫(yī)學(xué)院大學(xué)生科研基金(No.WK2014S19)~~
【分類號(hào)】：R38
【正文快照】： 標(biāo)本制作是寄生蟲實(shí)驗(yàn)技術(shù)中基礎(chǔ)而重要的工作。單染法常被應(yīng)用于寄生蟲學(xué)的教學(xué)和科研工作中[1-3],其制作技術(shù)已經(jīng)比較成熟和普遍。復(fù)染法利用不同染料之間的性質(zhì)差異,對(duì)不同組織的吸附性能不同,使得蟲體各器官和組織之間產(chǎn)生色差,使蟲體組織的立體感增強(qiáng),便于觀察。目前已有

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