本文關(guān)鍵詞： 約氏瘧原蟲 紅內(nèi)期 培養(yǎng) 體外　出處：《中國(guó)寄生蟲學(xué)與寄生蟲病雜志》2017年04期 　論文類型：期刊論文

【摘要】：約氏瘧原蟲(Plasmodium yoelii)紅內(nèi)期的體外連續(xù)培養(yǎng)尚未取得理想的效果。本研究在Trager等體外培養(yǎng)惡性瘧原蟲方法的基礎(chǔ)上,對(duì)培養(yǎng)條件進(jìn)行改進(jìn):將約氏瘧原蟲放在懸液系統(tǒng)培養(yǎng)儀的小室內(nèi),使用磁性攪拌器攪拌成懸液;每天更換培養(yǎng)基并向培養(yǎng)基中添加新的網(wǎng)織紅細(xì)胞;37℃、5% CO_2培養(yǎng)箱內(nèi)常規(guī)培養(yǎng)。每日取體外培養(yǎng)物尾靜脈注射小鼠,第3天檢查小鼠體內(nèi)瘧原蟲感染情況。結(jié)果顯示,體外培養(yǎng)的24 h內(nèi),約氏瘧原蟲的原蟲率增長(zhǎng)至2.200%,呈小幅增長(zhǎng),但隨后開(kāi)始下降。第10天,未發(fā)現(xiàn)被感染的紅細(xì)胞。第1~9天的體外培養(yǎng)物感染的小鼠均呈陽(yáng)性,且第10天的體外培養(yǎng)物感染小鼠呈陰性。第1天體外培養(yǎng)物感染小鼠的原蟲率最高,為54.960%。說(shuō)明約氏瘧原蟲紅內(nèi)期的體外培養(yǎng)可以維持9 d,且體外培養(yǎng)的約氏瘧原蟲具有感染力。
[Abstract]:Plasmodium yoelii. In vitro continuous culture of erythroid phase has not achieved ideal results. This study was based on Trager and other methods of in vitro culture of Plasmodium falciparum. The culture conditions were improved: Plasmodium yoelii was placed in the cell of the suspension system culture instrument, and the suspension was stirred with a magnetic agitator; The medium was changed every day and new reticulocyte was added to the medium. Normal culture was carried out in 37 鈩，

本文編號(hào)：1448307