本文關(guān)鍵詞： 帕金森 抑郁 補(bǔ)腎活血飲 -羥色胺 去甲腎上腺素 神經(jīng)肽Y　出處：《中國中西醫(yī)結(jié)合雜志》2017年11期 　論文類型：期刊論文

【摘要】：目的探討補(bǔ)腎活血飲對帕金森病伴發(fā)抑郁模型大鼠行為學(xué)及腦內(nèi)單胺類神經(jīng)遞質(zhì)5-羥色胺(5-HT)、去甲腎上腺素(NE)及神經(jīng)肽Y(NPY)的影響。方法采用直接向腦內(nèi)注入6-羥基多巴胺損毀腦黑質(zhì)致密部的方法建立帕金森病模型,并采用慢性不可預(yù)見性溫和應(yīng)激及孤養(yǎng)方法建立抑郁模型。將造模成功的75只PD大鼠用隨機(jī)數(shù)字表法分為模型組、補(bǔ)腎活血飲組、鹽酸氟西汀組、多巴絲肼組、鹽酸氟西汀+多巴絲肼組,每組15只;另取20只正常大鼠為正常組。通過糖水偏愛測試、強(qiáng)迫游泳實(shí)驗(yàn)、懸尾實(shí)驗(yàn)等方法檢測大鼠行為;采用高效液相-電化學(xué)檢測(HPLC-ECD)測定各組大鼠腦內(nèi)5-HT、NE含量;用放射免疫法測定各組大鼠腦組織及血清中NPY含量。結(jié)果與正常組比較,給藥前、治療后模型組大鼠體重明顯下降(P0.01);治療后,模型組大鼠懸尾靜止時(shí)間、強(qiáng)迫游泳不動時(shí)間均明顯延長,糖水偏愛率明顯減少,大鼠腦組織中5-HT及NE含量減少,血清及腦組織中NPY含量降低(均P0.01)。與模型組比較,治療后補(bǔ)腎活血飲組、鹽酸氟西汀組、鹽酸氟西汀+多巴絲肼組大鼠體重升高(P0.01),懸尾靜止時(shí)間、強(qiáng)迫游泳不動時(shí)間均減少(P0.01),糖水測試糖水偏愛率明顯增高(P0.01),血清及腦組織中NPY含量升高(P0.01);補(bǔ)腎活血飲組、鹽酸氟西汀組及鹽酸氟西汀+多巴絲肼組、多巴絲肼組大鼠腦組織中5-HT及NE含量均升高(P0.01,P0.05)。與多巴絲肼組比較,補(bǔ)腎活血飲組、鹽酸氟西汀組、鹽酸氟西汀+多巴絲肼組大鼠體重升高(P0.01),懸尾靜止時(shí)間、強(qiáng)迫游泳不動時(shí)間均減少(P0.05),糖水偏愛率增高(P0.05),血清及腦組織中NPY含量明顯升高(P0.01)。結(jié)論補(bǔ)腎活血飲能促進(jìn)PD伴發(fā)抑郁模型大鼠腦組織NPY釋放,增加5-HT、NE含量,改善帕金森大鼠抑郁樣行為。
[Abstract]:Objective to investigate the effects of Bushen Huoxue decoction (BHXY) on the behavior and brain monoamine neurotransmitter 5-hydroxytryptamine (5-HT) in rats with Parkinson's disease (PD) and depression. Methods Parkinson's disease model was established by direct injection of 6-hydroxydopamine into the brain to damage the substantia nigra compactness. The depression model was established by chronic unpredictable mild stress and solitary nourishment. 75 PD rats were randomly divided into three groups: model group, Bushen Huoxue Yin group and fluoxetine hydrochloride group. Dobutazid group, fluoxetine hydrochloride dobutazide group, 15 rats in each group; Another 20 normal rats were taken as normal group. The behavior of rats was detected by sugar water preference test, forced swimming test and tail suspension test. HPLC-ECD was used to detect the content of 5-HT NE in the brain of rats in each group by high performance liquid chromatography (HPLC) and electrochemical detection (HPLC-ECD). The contents of NPY in brain tissue and serum of rats in each group were measured by radioimmunoassay. After treatment, the rest time of tail suspension, the immobility time of forced swimming, the preference rate of sugar water and the contents of 5-HT and NE in brain tissue of the model group were significantly prolonged. The content of NPY in serum and brain tissue was lower than that in model group (P 0.01). Compared with model group, Bushen Huoxue Yin group and fluoxetine hydrochloride group after treatment were significantly lower than those in model group. In fluoxetine dobutazide group, the weight of rats increased P0.01, the rest time of tail suspension and the immobility time of forced swimming decreased P0.01). Sugar water test showed that the preference rate of sugar water was significantly higher than that of the control group (P 0.01), and the content of NPY in serum and brain tissue was higher than that in the control group (P 0.01). The contents of 5-HT and NE in brain tissue of rats in Bushen Huoxue Yin group, fluoxetine hydrochloride group, fluoxetine hydrochloride dobutazid group and dobutazid group were all increased (P0.01). Compared with dobutazid group, Bushen Huoxue decoction group, fluoxetine hydrochloride group, fluoxetine hydrochloride group increased body weight and suspended tail rest time. The immobility time of forced swimming decreased P0.05, and the preference rate of sucrose water increased P0.05). Conclusion Bushen Huoxue decoction can promote the release of NPY and increase the content of 5-HT NE in brain tissue of PD rats with depression. To improve the depressive behavior of Parkinson's rats.
【作者單位】： 中國人民解放軍總醫(yī)院中醫(yī)科;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No.81273969)
【分類號】：R285.5;R-332
【正文快照】： 抑郁癥(depression)是由各種原因引起的、慢性的以抑郁為主要癥狀的一組情感障礙綜合征。抑郁癥伴隨很多慢性病發(fā)生而發(fā)生,尤其很多老年慢性病,調(diào)查發(fā)現(xiàn)約30%~40%的帕金森病(Parkinson's disease,PD)患者伴發(fā)抑郁[1]。抑郁可以加重PD患者的認(rèn)知功能損害及運(yùn)動障礙,但由于其起

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