本文關(guān)鍵詞： 自體干細(xì)胞移植 免疫重建 淋巴細(xì)胞 穩(wěn)態(tài)增殖 細(xì)胞周期　出處：《汕頭大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：【目的】 1、分析自體造血干細(xì)胞移植(Autologous hematopoietic stem cell transplantation ,AHSCT)前外周血淋巴細(xì)胞亞群及細(xì)胞周期的情況。 2、分析AHSCT后外周血淋巴細(xì)胞亞群及細(xì)胞周期變化的規(guī)律,初步了解血液腫瘤AHSCT后外周血淋巴細(xì)胞的免疫重建。 【方法】 1、應(yīng)用流式細(xì)胞術(shù)(Flow cytometry,FCM)分析21例血液腫瘤患者AHSCT前后淋巴細(xì)胞亞群變化。 2、應(yīng)用FCM測定15例血液腫瘤患者AHSCT前后外周血淋巴細(xì)胞細(xì)胞周期變化。 【結(jié)果】 1、CD3~+CD4~+細(xì)胞在AHSCT前低于正常。淋巴細(xì)胞總數(shù)在AHSCT后1月明顯低于正常,在AHSCT后3月恢復(fù)正常,其各淋巴細(xì)胞亞群恢復(fù)時(shí)間不同,其中自然殺傷(Natural killer,NK)細(xì)胞、B細(xì)胞在3個(gè)月恢復(fù),T細(xì)胞AHSCT后9個(gè)月恢復(fù)正常,其中主要為CD3~+CD8~+細(xì)胞,CD3~+CD8~+細(xì)胞在AHSCT后1月有所下降,在3個(gè)月時(shí)恢復(fù)并高于正常,至9個(gè)月時(shí)逐漸下降至正常,而CD3~+CD4~+細(xì)胞在AHSCT后9個(gè)月仍然遠(yuǎn)低于正常,其恢復(fù)正常需要更長時(shí)間。 2、外周血淋巴細(xì)胞的DNA合成期細(xì)胞比率(S-phage fraction,SPF)、細(xì)胞凋亡率(Apoptosis,APO)在AHSCT前正常:AHSCT后SPF明顯高于正常,在AHSCT后9個(gè)月時(shí)恢復(fù)正常。Apo在AHSCT后1月時(shí)明顯高于移植前,但其迅速下降,在3個(gè)月時(shí)稍高于正常,其后降至正常。 【結(jié)論】 1、血液腫瘤患者AHSCT前存在淋巴細(xì)胞亞群異常,AHSCT后淋巴細(xì)胞總數(shù)、NK細(xì)胞、B細(xì)胞在AHSCT后早期較快恢復(fù),T細(xì)胞亞群中CD3~+CD8~+細(xì)胞恢復(fù)較早,而CD3~+CD4~+細(xì)胞恢復(fù)緩慢。 2、血液腫瘤患者AHSCT前外周血淋巴細(xì)胞細(xì)胞周期分布正常,AHSCT后淋巴細(xì)胞APO和SPF均明顯高于正常,其中APO在AHSCT后6月恢復(fù)正常,SPF在AHSCT后9月恢復(fù)正常。
[Abstract]:[Objective]
1, the peripheral blood lymphocyte subsets and cell cycle of autologous hematopoietic stem cell transplantation (Autologous hematopoietic stem cell transplantation, AHSCT) were analyzed.
2, the changes of lymphocyte subsets and cell cycle in peripheral blood after AHSCT were analyzed, and the immune reconstruction of peripheral blood lymphocytes in blood after AHSCT was preliminarily understood.
[method]
1, the changes of lymphocyte subsets before and after AHSCT in 21 blood tumor patients were analyzed by Flow cytometry (FCM).
2, the cell cycle changes in peripheral blood lymphocytes of 15 patients with hematologic tumor were measured by FCM.
[results]
1, CD3~+CD4~+ cells was lower than normal before AHSCT. The total number of lymphocytes in AHSCT after January was significantly lower than the normal, returned to normal at AHSCT after March, the lymphocyte subsets with different recovery time, the natural killer (Natural killer NK) cells, B cells in the 3 months of recovery, T cell AHSCT 9 months after recovery normally, including CD3~+CD8~+ cells, CD3~+CD8~+ cells in January AHSCT decreased after 3 months in recovery and higher than normal at 9 months decreased to normal, while CD3~+CD4~+ cells in AHSCT after 9 months is still far below normal, the recovery will take longer.
2, peripheral blood lymphocyte DNA synthesis cell ratio (S-phage fraction, SPF), cell apoptosis rate (Apoptosis, APO) in AHSCT normal before: SPF was significantly higher than that of normal AHSCT, AHSCT in the 9 months after the restoration of normal.Apo was significantly higher than that in AHSCT after January before transplantation, but it decreased rapidly. In 3 months, slightly higher than the normal, then returned to normal.
[Conclusion]
1, blood tumor patients had abnormal lymphocyte subsets before AHSCT. After AHSCT, the total number of lymphocytes, NK cells and B cells recovered rapidly at the early stage after AHSCT. The CD3~+CD8~+ cells in T cell subgroup recovered earlier, while CD3~+CD4~+ cells recovered slowly.
2, the cell cycle distribution of peripheral blood lymphocytes was normal in patients with hematological malignancies before AHSCT. After AHSCT, APO and SPF in lymphocytes were significantly higher than those in normal ones. APO returned to normal after AHSCT in June, and SPF returned to normal in September.

【學(xué)位授予單位】：汕頭大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R392

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