發(fā)布時(shí)間：2018-01-19 02:06

  本文關(guān)鍵詞： 型糖尿病 視網(wǎng)膜病變 Lp-PLA ZDF大鼠　出處：《中國(guó)比較醫(yī)學(xué)雜志》2017年10期 　論文類型：期刊論文

【摘要】：目的觀察丹酚酸A(SAA)對(duì)Zucker糖尿病肥胖(ZDF)大鼠視網(wǎng)膜病變的干預(yù)作用,探討SAA可能的作用機(jī)制。方法取7~8周齡雄性ZDF大鼠32只,Purina#5008飼料飼喂4周后,按血糖平均分為模型對(duì)照組,SAA高、低劑量組與二甲雙胍(Met)組,每組8只。另取同齡雄性ZL大鼠8只作為正常對(duì)照。SAA低、高劑量組每日尾靜脈注射SAA 0.5 mg/kg、1 mg/kg,Met組每日經(jīng)口灌胃Met 200 mg/kg,連續(xù)給藥12周,觀察ZDF大鼠白內(nèi)障發(fā)生率和視網(wǎng)膜病理學(xué)改變,并檢測(cè)血糖血脂、糖化血紅蛋白(Hb A1c)和血清白介素1(IL-1)、IL-6、氧化性低密度脂蛋白(ox-LDL)、丙二醛(MDA)含量和脂蛋白相關(guān)磷脂酶A2(Lp-PLA2)的活性,以及尿液中轉(zhuǎn)鐵蛋白(TRF)、視黃醇結(jié)合蛋白(RBP)的含量。結(jié)果模型對(duì)照組大鼠血糖、血脂及Hb A1c均上升,糖尿病性白內(nèi)障發(fā)病率68.75%,視網(wǎng)膜基底增厚和微血管病變,血清IL-1、IL-6、ox-LDL和MDA含量和Lp-PLA2活性顯著升高,尿液中TRF和RBP含量亦顯著升高。給予SAA后,ZDF大鼠白內(nèi)障發(fā)病率降低,視網(wǎng)膜病理學(xué)形態(tài)有不同程度的改善,血清IL-1、IL-6、ox-LDL、MDA含量和Lp-PLA2活性下降,尿液中TRF、RBP含量亦下降。結(jié)論 SAA能改善ZDF大鼠糖尿病視網(wǎng)膜病變,降低白內(nèi)障發(fā)生率,其作用途徑可能與抑制慢性炎癥、防止脂質(zhì)過氧化和降低Lp-PLA2活性有關(guān)。
[Abstract]:Objective to observe the effect of Salvianolic acid (SAA) on retinopathy in Zucker diabetic obese rats. To explore the possible mechanism of SAA. Methods 32 male ZDF rats aged 7 to 8 weeks were fed with the feed of Purina #5008 for 4 weeks, and were divided into model control group according to blood glucose. Low dose group and metformin metformin group, 8 rats in each group. Another 8 male ZL rats of the same age were taken as normal control. SAA was low, and SAA 0.5 mg/kg was injected daily in high dose group. One mg / kg Met group was given orally Met 200mg / kg daily for 12 weeks to observe the incidence of cataract and pathological changes of retina in ZDF rats. Blood glucose, blood lipids, glycosylated hemoglobin (HbA1c), interleukin-1 (IL-1) and interleukin-6 (IL-6), oxidizing low density lipoprotein (ox-LDL) were measured. The content of malondialdehyde (MDA) and the activity of lipoprotein associated phospholipase A _ 2 (Lp-PLA _ 2), as well as the urine transferrin (TRFs). Results the levels of blood glucose, blood lipid and HB A1c in the model control group were increased, and the incidence of diabetic cataract was 68.75%. The level of IL-6 ox-LDL and MDA and the activity of Lp-PLA2 were significantly increased in patients with retinal basement thickening and microangiopathy. The contents of TRF and RBP in urine were also significantly increased. The incidence of cataract was decreased and the pathological morphology of retina was improved in different degree. The content of Lp-PLA2 and the content of Lp-PLA2 in urine decreased. Conclusion SAA can improve diabetic retinopathy in ZDF rats. Reducing the incidence of cataract may be related to the inhibition of chronic inflammation, the prevention of lipid peroxidation and the reduction of Lp-PLA2 activity.
【作者單位】： 浙江中醫(yī)藥大學(xué)動(dòng)物實(shí)驗(yàn)研究中心/比較醫(yī)學(xué)研究所;溫州醫(yī)科大學(xué)附屬第二醫(yī)院;
【基金】：浙江省中醫(yī)藥管理局重點(diǎn)項(xiàng)目(2015ZZ009) 浙江中醫(yī)藥大學(xué)比較醫(yī)學(xué)創(chuàng)新團(tuán)隊(duì)(XTD201301)
【分類號(hào)】：R285.5;R-332
【正文快照】： 2.溫州醫(yī)科大學(xué)附屬第二醫(yī)院,浙江溫州325027)and retinal pathology was observed,and levels of GLU,TC and Hb A1cin blood,transferrin(TRF)and retinol bindingprotein(RBP)in urine and levels of interleukin-1(IL-1),interleukin-6(IL-6),oxidized low density lipopr

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