本文關(guān)鍵詞：BCR組庫分析乙肝疫苗接種反應(yīng)及初步機(jī)理分析　出處：《免疫學(xué)雜志》2017年09期 　論文類型：期刊論文

  更多相關(guān)文章： B細(xì)胞受體 免疫組庫 乙肝疫苗 高通量測序

【摘要】：目的接種乙肝疫苗是控制乙肝流行最經(jīng)濟(jì)有效的方法,通過對3次乙肝注射疫苗前后的B細(xì)胞庫進(jìn)行高通量測序分析,探討注射乙肝疫苗后體液免疫的動態(tài)過程。方法將志愿者接種乙肝疫苗前后的外周血樣進(jìn)行密度梯度離心獲得淋巴細(xì)胞并提取RNA,逆轉(zhuǎn)錄為cDNA后對B細(xì)胞受體H鏈的CDR3區(qū)序列進(jìn)行高通量測序,以揭示注射乙肝疫苗前后B細(xì)胞應(yīng)答的變化,分析人外周血B細(xì)胞受體CDR3的多樣性以及序列變化與疫苗應(yīng)答之間的關(guān)系。結(jié)果志愿者外周血B細(xì)胞受體CDR3的種類和數(shù)量在接種疫苗后都有所降低,并表現(xiàn)出其特有的免疫學(xué)特征,包括高頻取用的V、D、J家族、V-J家族配對發(fā)生了改變,CDR3區(qū)氨基酸的取用和長度分布也發(fā)生顯著變化;并且接種疫苗后,志愿者外周血中存在某些寡克隆異常增殖的情況。結(jié)論大部分志愿者在接種疫苗后,B細(xì)胞受體組庫的多樣性等發(fā)生特征性改變,并且出現(xiàn)某些疫苗接種相關(guān)的寡克隆。B細(xì)胞受體高通量測序技術(shù)可以從克隆水平上監(jiān)控乙肝疫苗接種后抗體產(chǎn)生和表達(dá)的動態(tài)變化過程。
[Abstract]:Objective inoculation of hepatitis B vaccine is the most economical and effective method to control the epidemic of hepatitis B. High-throughput sequencing analysis was carried out on the B cell bank before and after three times of hepatitis B vaccine injection. Objective: to investigate the dynamic process of humoral immunity after hepatitis B vaccine injection. Methods Peripheral blood samples from volunteers before and after hepatitis B vaccine were centrifuged with density gradient centrifugation to obtain lymphocytes and extract RNA. In order to reveal the changes of B cell response before and after injection of hepatitis B vaccine, high-throughput sequencing of the CDR3 region of B cell receptor H chain was carried out after reverse transcription into cDNA. The diversity of B cell receptor CDR3 in human peripheral blood and the relationship between the sequence change and vaccine response were analyzed. Results the type and number of B cell receptor CDR3 in peripheral blood of volunteers were decreased after vaccination. . It also showed its unique immunological characteristics, including the changes in the amino acid uptake and length distribution of the CDR3 domain. And after vaccination, there were abnormal proliferation of some oligoclonal clones in peripheral blood of volunteers. Conclusion the diversity of B cell receptor group library in most volunteers after inoculation has some characteristic changes. Moreover, some vaccine-related oligoclonal. B cell receptor high-throughput sequencing techniques can monitor the dynamic process of antibody production and expression after hepatitis B vaccine inoculation at clone level.
【作者單位】： 第三軍醫(yī)大學(xué)全軍免疫學(xué)研究所;第三軍醫(yī)大學(xué)衛(wèi)生處;
【基金】：國家自然科學(xué)基金國際(地區(qū))合作與交流項(xiàng)目(81220108024)
【分類號】：R392-33
【正文快照】： 乙肝(viral hepatitis B)是由HBV引起的病毒性肝炎,防治乙肝仍是個世界性的問題[1]。在乙肝病毒感染預(yù)防中,接種疫苗是最安全有效的措施。疫苗接種后,可以刺激機(jī)體的免疫系統(tǒng)產(chǎn)生獲得性免疫并形成免疫記憶,從而在較長時間內(nèi),甚至終生對其(81220108024)產(chǎn)生免疫[1-2]。獲得性免，

本文編號：1439052