本文關(guān)鍵詞：培養(yǎng)體系對(duì)維持人臍帶間充質(zhì)干細(xì)胞特性及其體外擴(kuò)增效率的影響　出處：《中國(guó)組織工程研究》2017年13期 　論文類型：期刊論文

  更多相關(guān)文章： 干細(xì)胞 臍帶 間質(zhì)干細(xì)胞 組織工程 臍帶臍血干細(xì)胞 間充質(zhì)干細(xì)胞 培養(yǎng)體系 培養(yǎng)基 篩選 形態(tài) 表型 分化 擴(kuò)增

【摘要】：背景:前期數(shù)據(jù)顯示,應(yīng)用人血小板裂解液培養(yǎng)體系對(duì)人臍帶間充質(zhì)干細(xì)胞進(jìn)行體外培養(yǎng)時(shí),較無(wú)血清培養(yǎng)體系能更好地維持干細(xì)胞特性,而無(wú)血清培養(yǎng)體系相較于人血小板裂解液培養(yǎng)體系能一定程度上提高間充質(zhì)干細(xì)胞的體外增殖能力。目的:篩選能最大限度維持人臍帶間充質(zhì)干細(xì)胞特性及擴(kuò)增效率高的培養(yǎng)體系。方法:將6份人臍帶標(biāo)本分別接種于6種培養(yǎng)體系中,進(jìn)行臍帶間充質(zhì)干細(xì)胞原代培養(yǎng),6種培養(yǎng)體系分別為MesenC ult無(wú)血清完全培養(yǎng)基中添加2%人血小板裂解液(A組)、StemP ro無(wú)血清完全培養(yǎng)基中添加2%人血小板裂解液(B組)、MesenC ult無(wú)血清完全培養(yǎng)基(C組)、Stem Pro無(wú)血清完全培養(yǎng)基(D組)、低糖DMEM中添加體積分?jǐn)?shù)10%胎牛血清(E組)、低糖DMEM中添加10%人血小板裂解液(F組),14 d后進(jìn)行消化傳代培養(yǎng),比較各培養(yǎng)體系對(duì)人臍帶間充質(zhì)干細(xì)胞形態(tài)、表面標(biāo)記物、分化及增殖能力的影響。結(jié)果與結(jié)論:(1)D組第3代細(xì)胞細(xì)長(zhǎng),大小不均;E、F組第3代細(xì)胞扁平,其余組第3代細(xì)胞為長(zhǎng)梭形且大小較均一。A、B組第5代細(xì)胞形態(tài)及大小較第3代時(shí)無(wú)明顯變化,C組第5代細(xì)胞趨于扁平且大小不均一,D組第5代細(xì)胞形態(tài)較第3代時(shí)細(xì)長(zhǎng),E組第5代細(xì)胞形態(tài)較第3代時(shí)扁平,F組第5代細(xì)胞形態(tài)較第3代時(shí)無(wú)明顯變化;(2)各組細(xì)胞表面標(biāo)志物檢測(cè)結(jié)果無(wú)明顯區(qū)別;(3)A、B組成脂、成骨誘導(dǎo)率較高,E、F組次之,C、D組最低;(4)A組各代次細(xì)胞擴(kuò)增數(shù)量顯著高于C組,B組各代次細(xì)胞擴(kuò)增數(shù)量顯著高于D組,E、F組擴(kuò)增數(shù)量顯著低于培養(yǎng)組A、B組;(5)結(jié)果表明,無(wú)血清和人血小板裂解液相結(jié)合的培養(yǎng)基能較好地維持間充質(zhì)干細(xì)胞的特性及擴(kuò)增效率。
[Abstract]:Background: previous data showed that human umbilical cord mesenchymal stem cells cultured in vitro with human platelet lysate culture system can better maintain stem cell characteristics than serum-free culture system. Compared with human platelet lysate culture system, serum-free culture system can improve the proliferation of mesenchymal stem cells in vitro. Methods: 6 human umbilical cord specimens were inoculated into 6 culture systems. The primary culture system of umbilical cord mesenchymal stem cells was MesenC ult serum-free complete medium supplemented with 2% human platelet lysate (group A). 2% human platelet lysate group B was added to StemP's serum-free complete medium, and Mesen C ult was added to the complete serum-free medium (group C). Stem Pro serum-free complete medium was added to group D, low glucose DMEM added 10% fetal bovine serum to group E, and low glucose DMEM to 10% human platelet lysate F). The effects of different culture systems on morphology, surface marker, differentiation and proliferation of human umbilical cord mesenchymal stem cells were compared after 14 days. Uneven size; The cells of the third passage were flat in group E and F, and the cells of the third passage in the other groups were fusiform and the size of the cells in the fifth passage of group A B had no significant change compared with that in the third generation. The fifth passage of cells in group C tended to flatten and the size of group D was uneven. The morphology of the fifth passage of group D was more flat than that of group E at the third generation, and that of group E was more flat than that of group E at the third generation. In group F, there was no significant change in the morphology of the fifth passage compared with that in the third generation. 2) there was no significant difference in the detection results of cell surface markers in each group. The ratio of osteogenic induction was higher in group F than that in group C (P < 0.05). The number of expanded cells in group A was significantly higher than that in group C and group B was significantly higher than that in group D and group A was significantly lower than that in group Agna B. The results showed that the liquid phase binding medium without serum and human platelet cleavage could maintain the characteristics and amplification efficiency of mesenchymal stem cells.
【作者單位】： 青島市立醫(yī)院衛(wèi)生部細(xì)胞移植重點(diǎn)實(shí)驗(yàn)室臨床中心;
【分類號(hào)】：R329.2
【正文快照】： 文章快速閱讀:不同培養(yǎng)體系中人臍帶間充質(zhì)干細(xì)胞的特性及體外擴(kuò)增效率消化傳代培養(yǎng)結(jié)果表明:6組培養(yǎng)體系:原代培養(yǎng):14 d后:無(wú)血清和人血MesenC ult無(wú)血清完全培養(yǎng)基中添加2%人血小板裂解液;比較各培養(yǎng)體小板裂解液相臍帶間充質(zhì)StemP ro無(wú)血清完全培養(yǎng)基中添加2%人血小板裂解

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