本文關(guān)鍵詞：重組MUC1多肽及MUC1-MBP融合蛋白的純化及抗腫瘤作用比較研究　出處：《吉林大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： MUC1 MUC1-MBP MBP BCG 抗腫瘤

【摘要】：MUC1是粘蛋白家族中的一員，主要分布在腺上皮細(xì)胞及其來源的腫瘤細(xì)胞表面，因其在正常組織和腫瘤組織的差異使其成為腫瘤疫苗研究的有效靶點(diǎn)。以MUC1為基礎(chǔ)的疫苗已經(jīng)快速進(jìn)入人類的臨床試驗(yàn)，并且表現(xiàn)出良好的療效。本研究組成功制備出高純度的MUC1-MBP融合蛋白，初步研究表明，MUC1-MBP蛋白聯(lián)合BCG免疫在小鼠體內(nèi)能預(yù)防并抑制肺癌轉(zhuǎn)移及實(shí)體瘤的生長，，還能明顯抑制小鼠皮下人乳腺癌的生長，但對疫苗的各個(gè)組分MUC1、MBP、BCG的具體作用還沒有進(jìn)行深入的探討。本課題的研究目的是在研究組前期研究工作的基礎(chǔ)之上進(jìn)一步比較重組MUC1多肽及MUC1-MBP融合蛋白的抗腫瘤作用，分析MUC1多肽、MBP蛋白、BCG在免疫中所起的作用。 本研究通過IPTG誘導(dǎo)MUC1-MBP、MBP及MUC1-GST蛋白表達(dá)，采用Amylose resin親和層析制備高純度MUC1-MBP融合蛋白及MBP蛋白。用Glutathione Sepherose4B純化MUC1-GST，采用凝血酶裂解MUC1-GST，獲得重組MUC1多肽。用純化的蛋白在動(dòng)物腫瘤模型上進(jìn)行了研究，研究結(jié)果表明，MUC1-MBP聯(lián)合BCG具有顯著的抗腫瘤作用，而MUC1多肽聯(lián)合BCG沒有明顯的抗腫瘤作用，MUC1-MBP聯(lián)合BCG作為抗腫瘤疫苗其中的三個(gè)組分MUC1、MBP、BCG缺一不可。本研究結(jié)果進(jìn)一步提示了MUC1-MBP聯(lián)合BCG作為抗腫瘤疫苗的開發(fā)價(jià)值。
[Abstract]:MUC1 is a member of mucin family, mainly distributed on the surface of glandular epithelial cells and tumor cells derived from them. Because of its difference in normal tissue and tumor tissue, it has become an effective target of tumor vaccine research. The vaccine based on MUC1 has been rapidly introduced into human clinical trials. The high purity MUC1-MBP fusion protein was successfully prepared in this study. MUC1-MBP protein combined with BCG immunized mice can prevent and inhibit the metastasis of lung cancer and the growth of solid tumor, but also can significantly inhibit the growth of subcutaneous human breast cancer in mice. However, MBP was used as a component of the vaccine. The purpose of this study is to compare the recombinant MUC1 peptide and MUC1-MBP fusion protein on the basis of the previous research work of the study group. Antitumor effect. To analyze the role of MUC1 protein in immune system. In this study, the expression of MBP and MUC1-GST protein in MUC1-MBP was induced by IPTG. High purity MUC1-MBP fusion protein and MBP protein were prepared by Amylose resin affinity chromatography. Glutathione was used to prepare the fusion protein. MUC1-GST was purified by Sepherose4B. The recombinant MUC1 peptide was obtained by thrombin cleavage of MUC1-GST.The purified protein was used to study the animal tumor model. MUC1-MBP combined with BCG had significant anti-tumor effect, while MUC1 polypeptide combined with BCG had no obvious anti-tumor effect. MUC1-MBP and BCG were used as three components of MUC1MBP. The results of this study further suggest the value of MUC1-MBP combined with BCG as an anti-tumor vaccine.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R392.9

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本文編號(hào)：1417013