本文關(guān)鍵詞：人子宮內(nèi)膜癌裸鼠皮下移植瘤模型的建立　出處：《山東醫(yī)藥》2016年35期 　論文類型：期刊論文

  更多相關(guān)文章： 子宮內(nèi)膜癌 Ishikawa細(xì)胞株 子宮內(nèi)膜癌移植瘤 動(dòng)物模型

【摘要】：目的建立人子宮內(nèi)膜癌裸鼠皮下移植瘤模型。方法培養(yǎng)人子宮內(nèi)膜癌細(xì)胞系Ishikawa,制成腫瘤細(xì)胞懸液,于裸鼠(10只)右側(cè)肩胛背部皮下注射,每只裸鼠注射約1×107個(gè)細(xì)胞。接種后每日觀察裸鼠精神、活動(dòng)、飲食、排便等狀況及是否有腫瘤生長(zhǎng)、腫瘤生長(zhǎng)速度等。以接種部位可見或觸及腫瘤小結(jié)節(jié)為成瘤標(biāo)準(zhǔn),若第一次移植失敗則考慮行二次瘤細(xì)胞接種。發(fā)現(xiàn)腫瘤生長(zhǎng)后每3 d計(jì)算腫瘤體積。當(dāng)腫瘤最長(zhǎng)徑達(dá)15 mm或出現(xiàn)皮膚破潰時(shí),立即處死荷瘤裸鼠;否則于接種第49天將荷瘤裸鼠處死、剝出腫瘤,測(cè)量瘤體長(zhǎng)徑,稱取瘤體質(zhì)量。取腫瘤組織切片后行HE染色,觀察腫瘤組織病理變化。結(jié)果有1只裸鼠第一次接種失敗,在第二次接種后移植成功,一次成瘤率為9/10。在接種第40天和第46天各發(fā)現(xiàn)1只裸鼠瘤體表面皮膚破潰,發(fā)現(xiàn)后脫頸處死荷瘤裸鼠,測(cè)得腫瘤最長(zhǎng)徑分別為12.2、12.6 mm,腫瘤體積分別為505.14、510.30 mm3,腫瘤質(zhì)量分別為0.88、0.86 g;至接種第49天,存活的7只裸鼠移植瘤瘤體表面皮膚完整,無破潰缺損,處死裸鼠后完整剝離腫瘤,測(cè)得腫瘤最長(zhǎng)徑為11.7~14.0 mm,瘤重0.86~1.23 g。取移植瘤組織進(jìn)行病理檢查,腫瘤組織大體表現(xiàn)與鏡下表現(xiàn)符合人子宮內(nèi)膜癌的特點(diǎn)。結(jié)論采用將Ishikawa細(xì)胞株接種于裸鼠右側(cè)肩胛皮下的方式成功建立人子宮內(nèi)膜癌裸鼠皮下移植瘤模型,且移植瘤組織和細(xì)胞保留了人子宮內(nèi)膜癌的病理特征。
[Abstract]:Objective to establish a model of subcutaneous human endometrial carcinoma in nude mice. Methods cultured human endometrial carcinoma cell line Ishikawa into tumor cell suspension in nude mice (10) right scapular subcutaneous injection, each mouse injection of approximately 1 x 107 cells. After inoculation of mice were observed daily spirit, activity, diet, defecation other conditions and whether there is tumor growth, tumor growth rate. The inoculation site visible or palpable tumors into tumor nodules, if the first graft failure is considered for the two time of tumor cell inoculation. Found tumor growth every 3 d after the tumor volume was calculated. When the tumor diameter reached 15 mm or broken skin break when the nude mice were sacrificed immediately; otherwise the inoculation in nude mice were sacrificed forty-ninth days, stripping of tumor, measuring tumor size, weighing tumor weight. The tumor tissue sections were stained by HE staining to observe the pathological changes of tumor tissue. The results of 1 mice The first vaccination failure in second after inoculation of transplant success, a tumor was found 1 9/10. nude mice tumor surface skin ulceration after inoculation for fortieth days and forty-sixth days after the discovery of sacrificed mice, measured the tumor diameter were 12.2,12.6 mm, tumor volume integral don't 505.14510.30 mm3, tumor weights were 0.88,0.86 g; to forty-ninth days of inoculation, tumor of 7 nude mice body surface skin survived intact, no ulceration defect, the nude mice were killed after the tumor was removed, measured the tumor diameter of 11.7~14.0 mm, 0.86~1.23 g. and tumor weight of tumor tissue for pathological examination of tumor tissue. Gross appearance and endoscopic features in line with the characteristics of human endometrial carcinoma. Conclusion the Ishikawa cell line was inoculated subcutaneously in nude mice the right scapular model was successfully established subcutaneous human endometrial carcinoma in nude mice, and the transplanted tumor tissues and cells. The pathological features of endometrial carcinoma were retained.

【作者單位】： 青島市婦女兒童醫(yī)院;青島大學(xué);
【基金】：山東省優(yōu)秀中青年科學(xué)家科研獎(jiǎng)勵(lì)基金資助項(xiàng)目(BS2009SW002) 山東省自然科學(xué)基金資助項(xiàng)目(ZR2013HM012)
【分類號(hào)】：R737.33;R-332
【正文快照】： 子宮內(nèi)膜癌是女性生殖道三大惡性腫瘤之一,病死率僅次于卵巢癌,發(fā)病率逐年上升[1,2]。子宮內(nèi)膜樣腺癌是子宮內(nèi)膜癌最常見的病理類型,多見于絕經(jīng)后女性[3]。近年來對(duì)子宮內(nèi)膜癌的基礎(chǔ)與臨床研究發(fā)展迅速,建立合適的動(dòng)物模型對(duì)研究子宮內(nèi)膜癌生物學(xué)行為及進(jìn)行藥物實(shí)驗(yàn)治療具有重，

本文編號(hào)：1407222