本文關(guān)鍵詞：外源性透明質(zhì)酸對兔骨髓間充質(zhì)干細胞定向分化為軟骨細胞的影響　出處：《青島大學》2011年碩士論文　論文類型：學位論文

  更多相關(guān)文章： 骨髓間充質(zhì)干細胞 軟骨細胞 透明質(zhì)酸 分化

【摘要】：背景：骨髓間充質(zhì)干細胞有多向分化潛能,在不同的誘導條件下可以分化成許多不同的組織,近年來已成為組織工程軟骨構(gòu)建過程中最常用的種子細胞。 目的：通過分析外源性透明質(zhì)酸對兔骨髓間充質(zhì)干細胞體外增殖及定向分化為軟骨細胞的影響,探討關(guān)節(jié)腔內(nèi)環(huán)境對骨髓間充質(zhì)干細胞的作用。 方法：全骨髓法+貼壁培養(yǎng)法分離培養(yǎng)兔骨髓間充質(zhì)干細胞,取第4代細胞用于實驗,實驗組細胞加入透明質(zhì)酸誘導液,以轉(zhuǎn)化生長因子β3誘導組作為陽性對照,陰性對照組加入常規(guī)培養(yǎng)液。分別于誘導后第7,14,21 d行甲苯胺藍染色檢測蛋白聚糖表達,免疫組化染色及RT-PCR檢測細胞Ⅱ型膠原表達。 結(jié)果：經(jīng)透明質(zhì)酸誘導后,細胞增殖速度減慢,由長梭形變?yōu)槎嘟切巍E圓形,細胞外基質(zhì)呈甲苯胺藍異染性和Ⅱ型膠原免疫組化陽性, RT-PCR檢測示Ⅱ型膠原mRNA表達陽性,表現(xiàn)出軟骨細胞的分化特點,但表達均比陽性對照組弱。 結(jié)論：外源性透明質(zhì)酸具有誘導兔骨髓間充質(zhì)干細胞向軟骨細胞分化的能力,但比轉(zhuǎn)化生長因子β3的誘導能力弱。結(jié)果提示,關(guān)節(jié)腔內(nèi)環(huán)境對骨髓間充質(zhì)干細胞向軟骨細胞分化有正性促進作用,支持透明質(zhì)酸作為軟骨組織工程基質(zhì)使用。
[Abstract]:Background: bone marrow mesenchymal stem cells (BMSCs) have the potential to differentiate into many different tissues under different induction conditions and have become the most commonly used seed cells in the process of tissue engineering cartilage construction in recent years. Aim: to investigate the effects of exogenous hyaluronic acid on the proliferation and differentiation of rabbit bone marrow mesenchymal stem cells into chondrocytes in vitro. Methods: the rabbit bone marrow mesenchymal stem cells were isolated and cultured by whole bone marrow adherent culture method. The cells of the 4th passage were used in the experiment. The cells in the experimental group were added hyaluronic acid inducer. Transforming growth factor 尾 3 group was used as positive control group and negative control group was added into conventional culture medium. Toluidine blue staining was used to detect proteoglycan expression on day 71421 after induction. The expression of type 鈪，

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