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  本文關(guān)鍵詞：側(cè)腦室注射apelin-13抑制小鼠遠(yuǎn)端結(jié)腸運(yùn)動　出處：《蘭州大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： Apelin-13 APJ受體 阿片受體 排便 結(jié)腸排珠 離體結(jié)腸收縮

【摘要】：Apelin及其受體APJ廣泛分布于中樞神經(jīng)系統(tǒng)和外周組織。在周圍組織包括脾、胸腺、心臟、乳腺、胃、小腸和結(jié)腸等多個器官內(nèi)都能檢測到APJ受體和apelin的mRNA。在中樞神經(jīng)系統(tǒng),APJ以及apelin的mRNA主要分布于下丘腦、丘腦、大腦皮層和垂體等與內(nèi)臟運(yùn)動緊密相關(guān)的腦區(qū)。并且apelin對多種機(jī)體的生理和病理活動具有調(diào)節(jié)作用,包括心血管、免疫、神經(jīng)內(nèi)分泌、體液平衡和攝食等。許多研究結(jié)果表明攝食與胃腸運(yùn)動功能關(guān)系密切,提示apelin可能與胃腸運(yùn)動的調(diào)節(jié)有關(guān),然而目前尚未見相關(guān)報(bào)道。 本研究旨在探討側(cè)腦室注射apelin-13對小鼠結(jié)腸運(yùn)動的影響以及apelin-13對離體結(jié)腸收縮的影響。側(cè)腦室注射apelin-13 (0.3,0.5,1和3μg/只)能夠劑量依賴的抑制結(jié)腸排珠和排便,說明apelin-13在中樞水平上參與結(jié)腸運(yùn)動的調(diào)控。為了探討apelin-13抑制結(jié)腸運(yùn)動的機(jī)制,我們選用APJ受體特異性拮抗劑apelin-13 (F13A)進(jìn)行研究,發(fā)現(xiàn)其能有效逆轉(zhuǎn)apelin-13的上述作用,說明APJ受體參與介導(dǎo)apelin-13對結(jié)腸運(yùn)動的抑制作用。為了進(jìn)一步探討apelin-13的作用機(jī)制,我們選取非選擇性阿片受體拮抗劑納洛酮,結(jié)果證明它也能夠完全阻斷apelin-13對結(jié)腸運(yùn)動的抑制作用,說明阿片受體也參與了這一調(diào)制過程。然而離體實(shí)驗(yàn)結(jié)果表明(10-8-10-6M) apelin-13并不能引起遠(yuǎn)端結(jié)腸肌條的收縮變化。
[Abstract]:Apelin and its receptor APJ are widely distributed in the central nervous system and peripheral tissues, including the spleen, thymus, heart, breast, stomach. APJ receptors and apelin mRNAs can be detected in many organs such as small intestine and colon. APJ and mRNA of apelin are mainly distributed in hypothalamus and thalamus in central nervous system. The cerebral cortex and pituitary are closely related to the visceral movement, and apelin can regulate the physiological and pathological activities of many organisms, including cardiovascular, immune, neuroendocrine. Many studies have shown that intake is closely related to gastrointestinal motility, suggesting that apelin may be involved in the regulation of gastrointestinal motility, but no related reports have been reported. The purpose of this study was to investigate the effects of intracerebroventricular injection of apelin-13 on colonic motility in mice and the effect of apelin-13 on colonic contraction in vitro. 0.3. 0. 5 渭 g / g and 3 渭 g / g) could inhibit colon droplet excretion and defecation in a dose-dependent manner. It is suggested that apelin-13 is involved in the regulation of colonic motility at the central level. In order to explore the mechanism of apelin-13 inhibiting colon motility. We selected apelin-13 F13A, a specific antagonist of APJ receptor, and found that it can effectively reverse the above effects of apelin-13. It is suggested that APJ receptor is involved in mediating the inhibitory effect of apelin-13 on colon motility. In order to further explore the mechanism of apelin-13. We selected naloxone, a non-selective opioid receptor antagonist, which proved that naloxone could also completely block the inhibition of colon motility by apelin-13. These results suggest that opioid receptors are also involved in this modulation process. However, in vitro, 10-8-10 ~ (-6) M) apelin-13 can not induce the contraction of distal colonic muscle strips.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

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本文編號：1405907