本文關(guān)鍵詞：不同時間點(diǎn)不同應(yīng)激方法致大鼠焦慮樣行為的研究　出處：《中國比較醫(yī)學(xué)雜志》2017年01期 　論文類型：期刊論文

  更多相關(guān)文章： 焦慮模型 空瓶應(yīng)激 慢性情緒應(yīng)激 慢性束縛應(yīng)激

【摘要】：目的從不同時間點(diǎn)比較幾種不同的應(yīng)激方法建立焦慮大鼠模型的生物學(xué)特點(diǎn),為尋找最合適的造模方法提供實驗依據(jù)。方法 70只大鼠隨機(jī)分為正常組、空瓶應(yīng)激組、慢性情緒應(yīng)激(chronic emotional stress,CES)組、束縛應(yīng)激3 h組、束縛應(yīng)激6 h組,分別進(jìn)行造模,于實驗第7 d、14 d、21 d分別采用高架十字迷宮和場景恐懼測試測定大鼠的焦慮樣行為,曠場測試考察大鼠的焦慮或抑郁樣行為,強(qiáng)迫游泳實驗測定大鼠的抑郁樣行為,采用Elisa試劑盒檢測大鼠海馬組織中5-HT、DA含量。結(jié)果焦慮樣行為學(xué)測試結(jié)果表明,第14天起,空瓶應(yīng)激組、CES組、束縛應(yīng)激6 h組大鼠開始產(chǎn)生焦慮樣行為,此后焦慮樣行為愈發(fā)明顯,強(qiáng)迫游泳實驗結(jié)果表明,束縛6 h組大鼠的不動時間于第7 d起即顯著增加(P0.05)。同時,與正常大鼠比較,空瓶應(yīng)激組和CES組大鼠海馬5-HT、DA含量于第14天開始顯著上升(P0.05或P0.01)。結(jié)論在上述幾種焦慮模型中,束縛應(yīng)激3 h焦慮樣行為不明顯;束縛應(yīng)激6 h可能由于應(yīng)激時間過長,表現(xiàn)出抑郁樣行為。空瓶應(yīng)激和CES能成功建立焦慮大鼠模型,且其焦慮行為表征有一定差異,可根據(jù)不同的實驗?zāi)康倪x擇對應(yīng)的模型方法。
[Abstract]:The biological characteristics of stress from different time points to compare different anxiety to establish rat model, to find the most suitable model to provide experimental basis. Methods 70 rats were randomly divided into normal group, empty bottle stress group, chronic emotional stress (chronic emotional, stress, CES) group, 3 h restraint stress 6 h group, restraint stress group, respectively. The model was made on the seventh D, 14 d and 21 d respectively with anxiety like behavior and elevated plus maze test were measured at the scene of fear, open field tests in rats with anxiety or depression like behavior, depression like behavior in rats by forced swimming experiment using 5-HT, Elisa kit, tissue of rat hippocampus. The detection of DA content in anxiety like behavior test results show that, the fourteenth day, the empty bottle stress group, CES group, 6 h restraint stress rats began to produce anxiety like behavior, anxiety like behavior after the more obvious Forced swimming, the experimental results show that the immobility time bound 6 h rats in seventh D increased significantly (P0.05). At the same time, compared with the normal rats, the empty bottle stress group and CES in hippocampus of rats in 5-HT group, the content of DA on the fourteenth day increased significantly (P0.05 or P0.01). In conclusion the several kinds of anxiety model, restraint stress 3 h anxiety like behavior is not obvious; restraint stress may be due to stress 6 h long time, showed depression like behavior. Empty bottle stress and CES can successfully establish rat models of anxiety, and the anxiety behavior is different, according to different objectives selection model the corresponding.

【作者單位】： 湖南中醫(yī)藥大學(xué)湖南省中藥粉體與創(chuàng)新藥物省部共建國家重點(diǎn)實驗室培育基地;湖南中醫(yī)藥大學(xué)第一附屬醫(yī)院;
【基金】：湖南省科技廳重點(diǎn)項目(2015DK3003) 湖南省研究生創(chuàng)新課題(CX2016B371;2016CX14)
【分類號】：R749.72;R-332
【正文快照】： was becoming increasingly apparent.Forced swimming test results showed that immobility time in 6 h restraint rats wassignificantly increased in the first 7 d(P0.05).Meanwhile,compared with control group,hippocampal 5-HT,DAcontents in empty bottle stress

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