本文關(guān)鍵詞：體外激活的小膠質(zhì)細(xì)胞對(duì)神經(jīng)元細(xì)胞的作用研究　出處：《北京協(xié)和醫(yī)學(xué)院》2011年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 小膠質(zhì)細(xì)胞 激活 神經(jīng)元 丁基苯酞

【摘要】：目的1.建立和完善大鼠腦皮質(zhì)小膠質(zhì)細(xì)胞原代培養(yǎng)的實(shí)驗(yàn)方法和條件；2.探索原代培養(yǎng)的小膠質(zhì)細(xì)胞經(jīng)脂多糖(LPS)體外激活后對(duì)神經(jīng)元細(xì)胞的影響；3.通過丁基苯酞(NBP)對(duì)小膠質(zhì)細(xì)胞激活過程的影響,探討國家Ⅰ類新藥NBP可能的新的作用靶點(diǎn)。 方法原代培養(yǎng)大鼠腦皮質(zhì)小膠質(zhì)細(xì)胞和神經(jīng)元細(xì)胞；利用LPS刺激小膠質(zhì)細(xì)胞,建立原代培養(yǎng)的小膠質(zhì)細(xì)胞體外激活的細(xì)胞模型；提取LPS激活的小膠質(zhì)細(xì)胞的培養(yǎng)基上清液作用于神經(jīng)元細(xì)胞,通過四甲基偶氮唑鹽(MTT)檢測(cè)神經(jīng)元細(xì)胞的存活率：在加入LPS作用前用NBP處理小膠質(zhì)細(xì)胞,通過觀察小膠質(zhì)細(xì)胞的形態(tài),ELISA法定量檢測(cè)炎性因子IL-1β、TNF-α及IL-10的釋放,硝酸還原法檢測(cè)炎性介質(zhì)一氧化氮(NO),以及MTT檢測(cè)神經(jīng)元細(xì)胞經(jīng)其培養(yǎng)基上清液作用后的存活率,綜合評(píng)估NBP對(duì)小膠質(zhì)細(xì)胞激活的影響。 結(jié)果成功培養(yǎng)出數(shù)量多、純度高的大鼠腦皮質(zhì)小膠質(zhì)細(xì)胞：LPS激活的小膠質(zhì)細(xì)胞的培養(yǎng)基上清液可致神經(jīng)元細(xì)胞的存活率下降；NBP處理后的小膠質(zhì)細(xì)胞經(jīng)LPS作用后,仍呈現(xiàn)為激活形態(tài),其釋放的NO減少,IL-1β、TNF-α及IL-10的含量無明顯變化,經(jīng)其培養(yǎng)基上清液作用后的神經(jīng)元細(xì)胞存活率較對(duì)照組升高,且丁基苯酞的上述作用呈現(xiàn)一定的劑量依賴性。 結(jié)論激活的小膠質(zhì)細(xì)胞對(duì)神經(jīng)元細(xì)胞起毒性作用；NBP不能抑制小膠質(zhì)細(xì)胞的激活,但能通過抑制激活的小膠質(zhì)細(xì)胞生成N0,從而減輕激活的小膠質(zhì)細(xì)胞對(duì)神經(jīng)元細(xì)胞的毒性作用,并具有劑量依賴性。
[Abstract]:Objective 1 . To establish and improve the experimental methods and conditions of primary culture of microglial cells in rat cerebral cortex ; 2 . To explore the effect of primary cultured microglial cells on neuronal cells following activation of lipopolysaccharide ( LPS ) in vitro ; 3 . Effects of butylphthalide ( NBP ) on the activation of microglial cells . Methods Primary cultured rat cortical microglial cells and neuronal cells were cultured in primary culture . The cells were stimulated with LPS to stimulate microglial cells . The cell survival rate of neurons was determined by MTT assay . The effects of NBP on the activation of microglial cells were assessed by means of MTT assay . The effects of NBP on the activation of microglial cells were assessed by MTT assay . Results The survival rate of the neurons was decreased after the LPS - activated microglial cells were activated by LPS . The survival rate of the neurons after NBP was decreased , the survival rate of IL - 1尾 , TNF - 偽 and IL - 10 increased , and the survival rate of the neurons after the supernatant was increased compared with the control group , and the above - mentioned effects of butylphthalide showed a dose - dependent manner . Conclusion The activated microglial cells play a toxic role in neuronal cells . NBP does not inhibit the activation of microglial cells , but can inhibit the activation of microglial cells to produce N0 , thus reducing the toxicity of activated microglial cells to neuronal cells and dose - dependent .

【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R329.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 胡丹波,李舜偉;慢性間斷性缺氧后細(xì)胞色素C和caspase-3的表螄達(dá)及維生素E和左旋丁基苯酞對(duì)其變化的影響[J];北京醫(yī)學(xué);2005年01期

2 吳艷芝;沈瑞樂;郭鐵;滕軍放;;丁基苯酞對(duì)老齡大鼠慢性腦缺血后HO-1和VEGF表達(dá)的影響[J];中國實(shí)用神經(jīng)疾病雜志;2007年04期

3 郭鐵;沈瑞樂;吳艷芝;滕軍放;;丁基苯酞對(duì)慢性腦缺血老齡大鼠海馬中NMDA受體亞單位NR2B及突觸素表達(dá)的影響[J];中國實(shí)用神經(jīng)疾病雜志;2007年04期

4 孔雙艷;李其富;羊潔;何俐;;丁基苯酞對(duì)大鼠缺血腦組織NGF及BDNF表達(dá)的影響[J];四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年03期

5 李其富;孔雙艷;德吉曲宗;何俐;周東;;丁基苯酞對(duì)大鼠局灶缺血腦組織VEGF及bFGF表達(dá)的影響[J];四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2008年01期

6 張進(jìn);蔣青青;;dl-3-正丁基苯酞軟膠囊治療急性腦梗死[J];中國臨床神經(jīng)科學(xué);2006年03期

7 崔麗英,李舜偉,呂傳真,董強(qiáng),董鵬,史朗峰,黃鑒政,毛善英,張朝東,曹云鵬,聶瑩雪,王維治,梁慶成,楊春曉,董為偉,李光勤,彭國光,羅祖明,何俐,萬琪,韓軍良,黃如訓(xùn),李玲,李承宴,蒙振中;恩必普軟膠囊治療中度急性缺血性卒中的多中心開放臨床研究[J];中國腦血管病雜志;2005年03期

8 沈瑞樂;王興萍;滕軍放;盧宏;;丁基苯酞對(duì)慢性腦缺血大鼠海馬Chat蛋白表達(dá)的影響[J];山東醫(yī)藥;2008年04期

9 陳頌春;洪震;魏文石;;丁基苯酞軟膠囊治療缺血性腦血管疾病的臨床對(duì)照研究[J];實(shí)用全科醫(yī)學(xué);2006年06期

10 謝瑛;靳守華;周國平;李丹;任志宏;;dl-3-正丁基苯酞軟膠囊治療輕中度急性缺血性腦卒中的開放臨床研究[J];中國現(xiàn)代神經(jīng)疾病雜志;2006年03期

，

本文編號(hào)：1391411