本文關(guān)鍵詞：刺莧花粉過敏性評價(jià)研究　出處：《核農(nóng)學(xué)報(bào)》2017年10期 　論文類型：期刊論文

  更多相關(guān)文章： 刺莧花粉 超聲提取 動物模型 BALB/c小鼠 HE染色

【摘要】：刺莧是一種外來入侵植物,刺莧花粉現(xiàn)已成為我國華南地區(qū)的重要過敏原,且刺莧花粉變應(yīng)原易與其他過敏原交叉反應(yīng)。為研究刺莧花粉的過敏性,以刺莧花粉為研究對象對其進(jìn)行致敏原純化,再利用BALB/c小鼠進(jìn)行腹腔注射,致敏滴鼻激發(fā)建立BALB/c小鼠動物過敏評價(jià)模型。通過對小鼠鼻部癥狀觀察及評分,鼻腔分泌物涂片染色觀察和血清中小鼠組胺和Ig E含量的測定,以及對小鼠解剖后肺部HE染色觀察來確定小鼠的致敏劑量。結(jié)果表明,小鼠體內(nèi)Ig E含量隨注射劑量的增加呈線性增長的趨勢,在注射量為75μg時達(dá)到最大值;小鼠體內(nèi)組胺含量在低劑量注射時呈線性增長趨勢,在注射量達(dá)到38μg后組胺含量變化趨于平緩;75μg注射量可引起小鼠明顯的過敏反應(yīng),且導(dǎo)致小鼠肺部炎癥反應(yīng)。本研究對刺莧花粉進(jìn)行過敏性評價(jià)研究,為全面進(jìn)行外來入侵植物的健康風(fēng)險(xiǎn)評價(jià)做了一定補(bǔ)充。
[Abstract]:Amaranthus spinosus is a kind of alien invasive plants, Amaranthus spinosus has become China's Southern China region an important allergen, and Amaranthus spinosus pollen allergen and other allergens for allergic reactions. The cross research of Amaranthus spinosus, with Amaranthus spinosus as the research object were sensitized to the original purification and reuse of BALB/c mice intraperitoneal injection, intranasal sensitization induced BALB/c mice animal allergy evaluation model. Through the observation and mouse nasal symptoms score, and serum were observed in mice of histamine and Ig content of E staining in nasal secretions, and the staining of lung in HE mice after dissection to determine the dose of sensitized mice. The results showed that the content of Ig E mice with linearly increasing dose, the injection amount of 75 g reached the maximum value; the content of histamine in mice showed a linear increasing trend in the low dose of injection, the injection amount of At 38 G after histamine content changes slowly; 75 g injection volume can cause allergic reactions in mice significantly, and cause lung inflammation in mice. The research on the evaluation of Amaranthus spinosus pollen allergy, do some supplement for health risk assessment of invasive plants comprehensively.

【作者單位】： 中國計(jì)量大學(xué)生命科學(xué)學(xué)院;浙江省海洋食品品質(zhì)及危害物控制技術(shù)重點(diǎn)實(shí)驗(yàn)室;海洋食品加工質(zhì)量控制技術(shù)與儀器國家地方聯(lián)合工程實(shí)驗(yàn)室;
【基金】：質(zhì)檢類公益項(xiàng)目(201410014-5)
【分類號】：R392
【正文快照】： 花粉主要由脂質(zhì)、多糖、蛋白質(zhì)及小分子化合物構(gòu)成,其中某些蛋白質(zhì)是致敏的主要成分�；ǚ圩鳛槲胄宰儜�(yīng)原進(jìn)入人體后可發(fā)生I型過敏反應(yīng),主要危害呼吸道,甚至引起肺部炎癥,對人體健康構(gòu)成巨大威脅。在花粉激發(fā)狀態(tài)下,效應(yīng)細(xì)胞釋放的生物活性介質(zhì)與相應(yīng)的受體發(fā)生特異性結(jié)合,，

本文編號：1385483