本文關(guān)鍵詞：內(nèi)嗅皮質(zhì)在不同方式應(yīng)激反應(yīng)中的作用研究　出處：《浙江大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 復(fù)合應(yīng)激 心理應(yīng)激 內(nèi)嗅皮質(zhì) 下丘腦室旁核 c-Fos 鵝膏氨酸

【摘要】：目的：探討內(nèi)嗅皮質(zhì)在急性復(fù)合應(yīng)激反應(yīng)和急性心理應(yīng)激反應(yīng)中的作用,并比較不同應(yīng)激源對下丘腦室旁核c-Fos表達(dá)的影響。材料和方法：1.實驗動物：健康雄性Sprague-Dawley (SD)大鼠,體重280±20g,每籠5只于浙江大學(xué)實驗動物中心常規(guī)飼養(yǎng)。2.實驗試劑：(1)鵝膏氨酸、臺盼藍(lán)；(2)兔抗鼠多克隆c-Fos抗體；(3)即用型SABC試劑盒,DAB底物試劑盒,正常山羊血清。3.實驗方法：所有實驗動物在飼養(yǎng)一周后隨機(jī)分為7組：正常對照組(N)、急性足底電擊組(F)、生理鹽水注射雙側(cè)內(nèi)嗅皮質(zhì)+急性足底電擊組(FC)、鵝膏氨酸損毀雙側(cè)內(nèi)嗅皮質(zhì)+急性足底電擊組(FS)、急性束縛應(yīng)激組(R)、生理鹽水注射雙側(cè)內(nèi)嗅皮質(zhì)+急性束縛應(yīng)激組(RC)、鵝膏氨酸損毀雙側(cè)內(nèi)嗅皮質(zhì)+急性束縛應(yīng)激組(RS)。用鵝膏氨酸化學(xué)損毀FS、RS組大鼠雙側(cè)內(nèi)嗅皮質(zhì),術(shù)后2周,對F組、FC組和FS組建立不可逃避的急性足底電擊應(yīng)激模型,對R組、RC組和RS組建立急性束縛應(yīng)激模型(N組大鼠不遭受應(yīng)激),采用c-Fos免疫組織化學(xué)方法,觀察急性足底電擊1小時后及急性束縛應(yīng)激1小時后下丘腦室旁核快反應(yīng)基因c-Fos的表達(dá)情況,并比較急性足底電擊應(yīng)激和急性束縛應(yīng)激對下丘腦室旁核c-Fos表達(dá)的影響。用SPSS16.0統(tǒng)計軟件對實驗數(shù)據(jù)進(jìn)行統(tǒng)計分析。c-Fos實驗數(shù)據(jù)采用非參數(shù)檢驗的Kruskal-Wallis H檢驗,以P0.05為判斷差異顯著性的標(biāo)準(zhǔn)。結(jié)果：(1)損毀大鼠雙側(cè)內(nèi)嗅皮質(zhì)能抑制不可逃避的急性足底電擊應(yīng)激反應(yīng)時PVN c-Fos的表達(dá)；(2)再次驗證損毀內(nèi)嗅皮質(zhì)能抑制急性束縛應(yīng)激時PVN中c-Fos的表達(dá)；(3)急性足底電擊應(yīng)激和急性束縛應(yīng)激誘導(dǎo)的PVN c-Fos的表達(dá)無顯著差異。結(jié)論：(1)不可逃避的急性足底電擊模型既有軀體應(yīng)激成分也有心理應(yīng)激成分,證明內(nèi)嗅皮質(zhì)參與急性復(fù)合性應(yīng)激反應(yīng)時HPA軸的功能調(diào)節(jié)。(2)束縛應(yīng)激作為一種較好的心理應(yīng)激模型,證明內(nèi)嗅皮質(zhì)參與急性心理應(yīng)激反應(yīng)時HPA軸的功能調(diào)節(jié)。結(jié)合內(nèi)嗅皮質(zhì)在復(fù)合應(yīng)激反應(yīng)中的作用,提示內(nèi)嗅皮質(zhì)至少參與厭惡性刺激的某些方面的信息處理過程,比如情緒部分。(3)不同應(yīng)激源誘導(dǎo)的PVN c-Fos的表達(dá)可能與應(yīng)激源的控制性有關(guān)；(4)綜合本課題組以往的研究,我們得出內(nèi)嗅皮質(zhì)功能與生理應(yīng)激和心理應(yīng)激均關(guān)系密切,但具體的調(diào)節(jié)機(jī)理有待進(jìn)一步研究。
[Abstract]:Objective: to investigate the role of endolfactory cortex in acute compound stress response and acute psychological stress response. The effects of different stress sources on the expression of c-Fos in hypothalamic paraventricular nucleus were compared. Materials and methods: 1. Experimental animal: healthy male Sprague-Dawley rats. The body weight was 280 鹵20g, 5 rats per cage were fed in Zhejiang University Experimental Animal Center. (2) Rabbit anti-mouse polyclonal c-Fos antibody; Methods: all the experimental animals were randomly divided into 7 groups after one week of feeding: normal control group (control group). In the acute plantar shock group, normal saline was injected into the bilateral olfactory cortex of the acute plantar shock group (FCX), and the acute plantar shock group (FSs) was damaged by geese oinanine. Acute restraint stress group, saline injection of bilateral olfactory cortex acute restraint stress group (RC3). The acute restraint stress group of bilateral olfactory cortex was damaged by geese oinanine, and the bilateral olfactory cortex of FSNRS group was damaged by chemical damage of geese ointment ammonia acid, 2 weeks after operation, the rats in group F were divided into two groups. FC group and FS group to establish an unavoidable acute plantar shock stress model, R group RC group and RS group to establish an acute restraint stress model N group rats did not suffer from stress). The expression of c-Fos in hypothalamic paraventricular nucleus was observed by c-Fos immunohistochemical method after 1 hour of acute plantar shock and 1 hour of acute restraint stress. The effects of acute plantar shock stress and acute restraint stress on the expression of c-Fos in hypothalamic paraventricular nucleus were compared. The experimental data were statistically analyzed by SPSS16.0 software. Kruskal-Wallis with nonparametric test. H test. Results the bilateral olfactory cortex lesion could inhibit the expression of PVN c-Fos during the inescapable acute plantar shock stress in rats. (2) it was proved that the lesion of olfactory cortex could inhibit the expression of c-Fos in PVN under acute restraint stress. (3) there was no significant difference in the expression of PVN c-Fos between acute plantar shock stress and acute restraint stress. The inescapable acute plantar shock model has both somatic and psychological stress components. It is demonstrated that the entorhinal cortex participates in the regulation of the function of HPA axis in acute complex stress response. 2) restraint stress is a better psychological stress model. It is demonstrated that the entorhinal cortex is involved in the regulation of the HPA axis in acute psychological stress response and the role of the entorhinal cortex in the complex stress response. The results suggest that the entorhinal cortex is involved in at least some aspects of the information processing of aversion stimulation. For example, the expression of PVN c-Fos induced by different stressors may be related to the control of stressors. 4) based on the previous studies of our research group, we conclude that the function of the entorhinal cortex is closely related to physiological stress and psychological stress, but the specific regulation mechanism needs to be further studied.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R363

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