本文關(guān)鍵詞：FSH和SCF對大鼠非梗阻性無精癥模型生精功能影響的初步研究　出處：《昆明醫(yī)學(xué)院》2011年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的：探討環(huán)磷酰胺和白消安不同劑量和不同用藥方式對大鼠生精功能的影響,尋求較好的制作大鼠NOA模型的方式。 方法：160只SPF級成年雄性Wistar大鼠隨機(jī)分為10組,每組16只,第1-3組分別予以白消安20mg.kg-1,15mg.kg-1,10mg.kg-1單次腹腔注射,第4-6組分別予以環(huán)磷酰胺200mg.kg-1,100mg.kg-1,75mg.kg-1單次腹腔注射,第7-9組分別以白消安40mg.kg-1,20mg.kg-1,環(huán)磷酰胺200mg.kg-1單次灌胃給藥,第10組予以生理鹽水2.Oml行單次腹腔注射。NOA模型成功的標(biāo)準(zhǔn)是用藥至大鼠的一個(gè)生殖周期約38天,大鼠睪丸、附睪組織切片找不到精子和精子細(xì)胞即為NOA模型成功。比較模型成功組和生理鹽水組,模型失敗組與生理鹽水組的大鼠體重,一側(cè)睪丸、附睪的重量及各級生精細(xì)胞數(shù)目統(tǒng)計(jì)學(xué)差異,結(jié)合大鼠的死亡率,得出實(shí)用的成模方式。 結(jié)果：用藥后38天第1組大鼠存活2只,第2組存活8只,第3組存活15只,第4組存活3只,第5組存活10只,第6組存活15只,第7組存活1只,第8組存活2只,第9組存活2只,生理鹽水組大鼠無死亡。用藥后38天,存活大鼠睪丸及附睪組織切片結(jié)果提示第1,2,7,8組達(dá)到NOA模型的標(biāo)準(zhǔn),其余各組大鼠NOA模型效果欠佳。通過對各組成模方式的比較,綜合模型成功且死亡率最低者為白消安15mg.kg-1單次腹腔注射是實(shí)用的Wistar大鼠NOA模型制作方式。 結(jié)論：白消安15mg.kg-1單次腹腔注射可成功制造Wistar大鼠NOA模型且死亡率較低。 目的：評估FSH、SCF對Wistar大鼠NOA動物模型生精功能的影響。 方法：利用白消安15mg. kg-1單次腹腔注射制作72只Wistar大鼠NOA動物模型,抽樣檢查模型結(jié)果成功后。將模型分為兩組：治療組以大鼠全身血FSH、SCF總量的5倍于大鼠后腿肌肉注射；對照組予以等量的生理鹽水行大鼠后腿肌肉注射；一周給藥兩次。給藥后19天、38天、57天分批取大鼠一側(cè)睪丸、附睪行組織切片,一側(cè)睪丸、附睪制作成一張切片。計(jì)算曲細(xì)精管垂直切面內(nèi)的精原細(xì)胞、精母細(xì)胞、精子細(xì)胞及精子的數(shù)量和垂直切面的附睪管內(nèi)精子數(shù)量,每張切片觀察符合標(biāo)準(zhǔn)的25個(gè)曲細(xì)精管。t檢驗(yàn)比較治療組和對照組不同用藥時(shí)間的結(jié)果,規(guī)定P0.01差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果：①治療組和對照組用藥19天后平均每個(gè)曲細(xì)精管的精原細(xì)胞數(shù)分別為58.00±15.70和30.90±13.52,P=0.000；精母細(xì)胞分別為47.44±15.52和12.65±8.08,P=0.000；精子細(xì)胞及精子數(shù)分別為12.42±8.11和0,P=0.005。實(shí)驗(yàn)組和對照組用藥19天后平均每個(gè)附睪管內(nèi)的精子數(shù)分別為0和0,P=0。②治療組和對照組用藥38天后平均每個(gè)曲細(xì)精管的精原細(xì)胞數(shù)分別為66.77±20.56和40.65±15.89,P=0.000；精母細(xì)胞分別為56.82±21.93和18.50±13.66,P=0.000；精子細(xì)胞及精子數(shù)分別為61.17±25.86和27.65±9.62,P=0.002。實(shí)驗(yàn)組和對照組用藥38天后平均每個(gè)附睪管內(nèi)的精子數(shù)分別為77.55±55.18和41.05±29.20,P=0.000。③治療組和對照組用藥57天后平均每個(gè)曲細(xì)精管的精原細(xì)胞數(shù)分別為78.63±24.28和47.95±18.12,P=0.000；精母細(xì)胞分別為68.53±21.54和29.60±15.13,P=0.000；精子細(xì)胞及精子數(shù)分別為102.83±35.31和45.15±20.96,P=0.000。治療組和對照組用藥19天后平均每個(gè)附睪管內(nèi)的精子數(shù)分別為122.13±80.67和58.50±26.15,P=0.000。 結(jié)論：肌注FSH和SCF能夠促進(jìn)Wistar大鼠NOA模型生精功能的恢復(fù)。
[Abstract]:Objective: To explore the effects of different doses of cyclophosphamide and Bai Xiao an on spermatogenesis in rats and to find a better way to make NOA model in rats.
Methods: 160 SPF adult male Wistar rats were randomly divided into 10 groups, 16 rats in each group, the 1-3 group were treated with busulfan 20mg.kg-1,15mg.kg-1,10mg.kg-1 single intraperitoneal injection, 4-6 group were given a single intraperitoneal injection of cyclophosphamide 200mg.kg-1100mg.kg-1,75mg.kg-1 and group 7-9 respectively with busulfan 40mg.kg-1,20mg.kg-1, cyclophosphamide 200mg.kg-1 single dose administration of Tenth 2.Oml normal saline group were given a single intraperitoneal injection of.NOA model for the standard of success is a reproductive cycle of medication to rats for about 38 days, the rat testis, epididymis tissue sections to find sperm and sperm cells is the NOA model successfully. Compared model group and saline group, failure model weight group and the saline group rats testicle, epididymis weight and all levels of spermatogenic cells in the number of statistically significant difference, with the mortality of rats, the mode into practical.
Results: after 38 days of first rats, 2, second groups of 8 rats survived, third groups of 15 rats survived, fourth groups of 3 rats survived, fifth groups of 10 rats survived, sixth groups of 15 rats survived, seventh groups of 1 rats survived, eighth groups of 2 rats survived, Ninth groups of 2 rats survived, saline group of rats died. 38 days after treatment, survival of rat testis and epididymis tissue results indicated that group 1,2,7,8 reached the standard NOA model, the poor the rats NOA model. Through the comparison of the mode, a comprehensive model of success and the lowest mortality for busulfan 15mg.kg-1 single intraperitoneal injection Wistar is a NOA rat model of practical production.
Conclusion: the single intraperitoneal injection of 15mg.kg-1 can make the NOA model of Wistar rats successfully and the mortality rate is low.
Objective: To evaluate the effect of FSH and SCF on the spermatogenic function of NOA animal model in Wistar rats.
Methods: using busulfan 15mg. kg-1 single intraperitoneal injection of 72 Wistar rats, NOA animal model, sampling inspection results after the success of model. The model is divided into two groups: treatment group in blood of FSH rats, SCF 5 times the total in rat hind leg muscle injection; the control group was given the same volume of saline for rat hind leg muscle injection; administered two times a week. For 19 days, 38 days after administration, the 57 batch of talent from rat testicle, epididymis side for tissue sections of testis, epididymis produced a slice. Calculation of the seminiferous tubules in the vertical section of spermatogonia, spermatocytes, the number and vertical section of the sperm cell and sperm of epididymis tube sperm, observe each slice 25 compliant seminiferous tubules.T test between the treatment group and control group in different time of medicine prescribed results, there were significant differences in P0.01.
Results: the treatment group and the control group of spermatogonial cell number after 19 days of treatment, the average of the seminiferous tubules were 58 + 15.70 and 30.90 + 13.52, P=0.000; spermatocytes were 47.44 + 15.52 and 12.65 + 8.08, P=0.000; sperm cells and sperm counts were 12.42 + 8.11 and 0, P= 0.005. the experimental group and the control group after 19 days of treatment the average sperm count in the epididymal duct were 0 and 0, P=0. in the treatment group and control group the number of spermatogonia after 38 days, the average of the seminiferous tubules were 66.77 + 20.56 and 40.65 + 15.89, P=0.000; spermatocytes were 56.82 and 21.93. 18.50 + 13.66, P=0.000; sperm cells and sperm counts were 61.17 + 25.86 and 27.65 + 9.62, P=0.002. experimental group and control group after 38 days of treatment the average sperm count in the epididymal duct were 77.55 + 55.18 and 41.05 + 29.20, P=0.000. of the treatment group and control group after 57 days of treatment The average number of spermatogonia in the seminiferous tubules were 78.63 + 24.28 and 47.95 + 18.12, P=0.000; spermatocytes were 68.53 + 21.54 and 29.60 + 15.13, P=0.000; sperm cells and sperm counts were 102.83 + 35.31 and 45.15 + 20.96, P=0.000. treatment group and control group after 19 days, the average the number of sperm in the epididymal duct were 122.13 + 80.67 and 58.50 + 26.15, P=0.000.
Conclusion: the intramuscular injection of FSH and SCF can promote the recovery of spermatogenesis in the NOA model of Wistar rats.

【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332;R698.2

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