本文關(guān)鍵詞：日本血吸蟲感染ICR小鼠后Tc1和Tc2細(xì)胞的動(dòng)態(tài)觀察及其功能分析　出處：《南京醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的觀察ICR小鼠感染日本血吸蟲后不同時(shí)期T淋巴細(xì)胞中殺傷性T細(xì)胞（Tc）亞型Tc1和Tc2比例的動(dòng)態(tài)變化，并分析兩者分別與Th1和Th2細(xì)胞比例變化的相關(guān)性。方法分別取日本血吸蟲感染0、3、5、8、13周的ICR小鼠脾臟淋巴細(xì)胞，采用流式細(xì)胞術(shù)檢測各個(gè)時(shí)期Tc1、Tc2和Th1、Th2細(xì)胞亞群分別占T淋巴細(xì)胞的比例。體外用SWA及SEA刺激小鼠脾臟淋巴細(xì)胞48h后，，采用流式細(xì)胞術(shù)檢測脾淋巴細(xì)胞中Tc1、Tc2細(xì)胞以及Th1、Th2細(xì)胞分別占T淋巴細(xì)胞的比例。結(jié)果與0周（未感染）對照比較，日本血吸蟲感染3、5、8、13周的小鼠T淋巴細(xì)胞中Tc1細(xì)胞比例顯著增高（P均0.01），感染5、8、13周的小鼠T淋巴細(xì)胞中Tc2細(xì)胞比例顯著增高（P均0.01）。比較Tc1和Tc2細(xì)胞比例在相同時(shí)間點(diǎn)的增速發(fā)現(xiàn)，感染血吸蟲后的前3周Tc1細(xì)胞比例增高最顯著，而感染3－5周內(nèi)Tc2細(xì)胞比例的增高最顯著。血吸蟲感染后各個(gè)時(shí)間點(diǎn)CD3~+T細(xì)胞中的Th1細(xì)胞比例與Tc1細(xì)胞比例平行增長，且兩者呈正相關(guān)（r=0.978，P=0.004）；CD3~+T細(xì)胞中的Th2細(xì)胞比例與Tc2細(xì)胞比例平行增長，且兩者呈正相關(guān)（r=0.974，P=0.005）。體外實(shí)驗(yàn)表明可溶性成蟲抗原（SWA）刺激脾細(xì)胞可優(yōu)勢增加T淋巴細(xì)胞中的Tc1細(xì)胞比例（P 0.01），而可溶性蟲卵抗原（SEA）刺激脾細(xì)胞可優(yōu)勢增加T淋巴細(xì)胞中的Tc2細(xì)胞比例（P 0.01）。結(jié)論日本血吸蟲感染不同階段，小鼠T淋巴細(xì)胞中的Tc1和Tc2細(xì)胞比例均顯著升高，并分別與Th1和Th2細(xì)胞比例變化呈正相關(guān)，根據(jù)兩者的增速，提示Tc1和Tc2分別可能促進(jìn)了Th1和Th2的分化。SWA可優(yōu)勢誘導(dǎo)Tc1細(xì)胞，而SEA可優(yōu)勢誘導(dǎo)Tc2細(xì)胞。
[Abstract]:Objective to observe the dynamic changes of Tc1 and Tc2 subtypes in T lymphocytes of ICR mice at different stages after infection with Schistosoma japonicum (Schistosoma japonicum). Methods the spleen lymphocytes of ICR mice infected with Schistosoma japonicum were collected for 813 weeks after infection with Schistosoma japonicum. The percentage of Tc1Tc2 and Th1Th2 cell subsets in T lymphocytes was detected by flow cytometry in each stage. After stimulation with SWA and SEA for 48 h in vitro, the ratio of Tc1Tc2 and Th1Th2 cells to T lymphocytes was measured. The percentage of Tc1tc2 cells and Th1Th 2 cells in splenic lymphocytes were detected by flow cytometry. The results were compared with those of 0 weeks (uninfected). The proportion of Tc1 cells in T lymphocytes of mice infected with Schistosoma japonicum for 813 weeks was significantly higher than that in mice infected with Schistosoma japonicum. The proportion of Tc2 cells in T lymphocytes of 13 weeks old mice increased significantly (P < 0.01). Comparing the growth rate of Tc1 and Tc2 cells at the same time, we found that the ratio of Tc1 cells to Tc2 cells increased at the same time. The proportion of Tc1 cells increased most significantly in the first three weeks after infection with Schistosoma japonicum. The proportion of Th1 cells in CD3 ~ T cells increased parallel to that of Tc1 cells at different time points after infection of Schistosoma japonicum. There was a positive correlation between them. The proportion of Th2 cells in CD3 ~ T cells increased in parallel with the proportion of Tc2 cells, and there was a positive correlation between them. In vitro experiments showed that SWA-induced splenocytes could increase the proportion of Tc1 cells in T lymphocytes (P 0.01). But soluble egg antigen (SEAA) can increase the proportion of Tc2 cells in T lymphocytes by stimulating spleen cells. Conclusion Schistosoma japonicum infection in different stages. The percentage of Tc1 and Tc2 cells in T lymphocytes of mice increased significantly, and was positively correlated with the changes of Th1 and Th2 cells, respectively, according to the growth rate of the two cells. These results suggested that Tc1 and Tc2 could promote the differentiation of Th1 and Th2, respectively. SWA could induce Tc1 cells preferentially, while SEA could induce Tc2 cells preferentially.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R392

【共引文獻(xiàn)】

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