本文關(guān)鍵詞：三種大鼠急性肺損傷模型的比較　出處：《成都醫(yī)學院學報》2016年01期 　論文類型：期刊論文

  更多相關(guān)文章： 急性肺損傷 盲腸結(jié)扎穿刺 腹腔注射 氣管滴注

【摘要】：目的比較腹腔注射脂多糖(lipopolysaccharide,LPS)、氣管滴注LPS和盲腸結(jié)扎穿刺(cecal ligation and puncture,CLP)誘導的3種急性肺損傷模型的差異。方法 70只成年SD大鼠隨機分為7組:正常組、腹腔注射LPS組(10mg/kg,LPS-IP組)及其假手術(shù)組(SHAM-1);氣管滴注LPS組(5mg/kg,LPS-IT組)及其假手術(shù)組(SHAM-2);盲腸結(jié)扎穿刺組(CLP)及其假手術(shù)組(SHAM-3)。于造模后24h,收集各組大鼠的動脈血用于血氣分析,并通過二喹啉甲酸(bicinchoninic acid,BCA)蛋白測定法、血細胞計數(shù)板以及瑞氏-姬姆薩染色分別對肺泡灌洗液中的蛋白含量、細胞總數(shù)和類別進行統(tǒng)計,同時利用濕干比和蘇木精-伊紅(HE)染色法來評價肺組織的損傷程度。結(jié)果 LPS-IT組和LPS-IP組的肺灌洗液總蛋白濃度、總細胞數(shù)均高于正常組和假手術(shù)組(P0.05),濕干比明顯增高(P0.05),PaO2低于正常組及相應的假手術(shù)組(P0.05);LPS-IT組和LPS-IP組肺組織有明顯病理變化,包括肺水腫和肺泡壁增厚,炎性細胞浸潤。CLP組未見明顯改變。結(jié)論在急性肺損傷的3種大鼠模型中,LPS-IT組和LPS-IP組肺損傷較為嚴重,CLP組未見明顯肺損傷。提示腹腔注射LPS及氣管給予LPS更適用于建立急性肺損傷模型以及后續(xù)應用藥物的動物實驗。
[Abstract]:Objective to compare the intraperitoneal injection of lipopolysaccharide (lipopolysaccharide, LPS), tracheal instillation of LPS and cecal ligation and puncture (cecal ligation and puncture, CLP) the difference between the 3 models of acute lung injury induced. Methods 70 adult SD rats were randomly divided into 7 groups: normal group, intraperitoneal injection of LPS group (10mg/kg group, LPS-IP) and the sham operation group (SHAM-1); tracheal instillation of LPS group (5mg/kg, group LPS-IT) and sham operation group (SHAM-2); cecal ligation and puncture (CLP) group and sham operation group (SHAM-3). After modeling 24h, blood gas analysis of arterial blood was collected for rats, and by two quinoline carboxylic acid (bicinchoninic acid BCA) protein determination method, blood cell counting plate and Giemsa staining respectively on alveolar lavage fluid protein content, cell number and categories of statistics, the ratio of wet and dry and hematoxylin eosin (HE) to evaluate the degree of injury of lung tissue staining results. The total protein concentration in bronchoalveolar lavage fluid of LPS-IT group and LPS-IP group, the total cell number was significantly higher than that in normal group and sham operation group (P0.05), wet dry ratio increased significantly (P0.05), PaO2 was lower than that in normal group and sham operation group (P0.05); the LPS-IT group and LPS-IP group had obvious pathological changes in lung tissue. Including pulmonary edema and alveolar wall thickening,.CLP group had no obvious change of infiltration of inflammatory cells. Conclusion in 3 kinds of rat model of acute lung injury in LPS-IT group and LPS-IP group were significantly more serious lung injury, lung injury was not found in the CLP. It is suggested that intraperitoneal injection of LPS and intratracheal instillation of LPS is more suitable for establishing the model of acute lung injury and the subsequent application of drugs in animal experiments.

【作者單位】： 成都醫(yī)學院藥學院;第三軍醫(yī)大學大坪醫(yī)院野戰(zhàn)外科研究所一室 創(chuàng)傷、燒傷與復合傷國家重點實驗室;
【基金】：國家重點基礎研究發(fā)展計劃973項目(No:2012CB518102) 軍隊“十二五”重點項目(No:BWS11J038)
【分類號】：R-332;R563
【正文快照】： 創(chuàng)傷、燒傷與復合傷國家重點實驗室(重慶400016)急性肺損傷(acute lung injury,ALI)由嚴重感染、休克、誤吸等肺內(nèi)外致病因素引發(fā),臨床主要表現(xiàn)為急性進行性加重的呼吸困難和難治性低氧血癥,嚴重者可發(fā)展為急性呼吸窘迫綜合征(acuterespiratory distress sydrome,ARDS)[1]。雖，

本文編號：1358749